Tissue culture and rapid propagation method of sugarcane

Abstract

The invention discloses a tissue culture and rapid propagation method of sugarcane, and relates to the technical field of plant tissue culture. The method comprises the following steps of selecting sugarcane axillary buds; washing the sugarcane axillary buds clean with clear water; sterilizing; inoculating the sugarcane axillary buds to a bud-inducing culture medium; regulating light and temperature; culturing until new buds are grown; transferring the new buds to a seedling culture medium; regulating the light and temperature to obtain seedlings; transferring the seedlings to a rooting culture medium; regulating the light and temperature; culturing to obtain sugarcane tissue cultured seedlings; carrying out seedling-hardening culture; and transplanting the hardened seedlings to an open field for cultivation. The tissue culture and rapid propagation method shortens a propagation period of the sugarcane.

Description

technical field

[0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of sugarcane. Background technique

[0002] Sugarcane is a plant of the Gramineae Saccharum genus and is an important sugar crop in the world. The cultivation of sugarcane is usually based on the axillary buds on the cane stem nodes, and the axillary buds on the stem base remaining in the soil are used as perennial root propagation. The propagation speed of these methods is slow, the genetic traits of the obtained sugarcane seedlings are quite different, and they are susceptible to virus infection during the cultivation process, resulting in serious degeneration of the varieties. Therefore, there is an urgent need to seek a method for high-yield, high-quality, efficient sugarcane propagation. Contents of the invention

[0003] The purpose of the present invention is to provide a method for rapid propagation of sug...

Examples

Embodiment 1

[0014] Take sugarcane axillary buds, wash them with clean water, soak them in 75% alcohol for 45s, then transfer them to 0.05% mercury chloride solution for 30 minutes, rinse them with sterile distilled water for 3 times; inoculate them into the bud induction medium, adjust the light to 1800LUX, and adjust the temperature Cultivate for 15 days at 23°C; when the new shoots grow to 2 cm, transfer to the medium for strong seedlings, adjust the light to 2000 LUX, and cultivate at 23°C for 18 days; when the shoots grow to 1 cm, transfer to the rooting medium. The light was adjusted to 2200 Lux, the temperature was 26°C, and the sugarcane tissue cultured seedlings were obtained by culturing for 12 days until 3 cm roots grew from the base of the seedlings; the seedlings were hardened and cultivated for 25 days, and finally transplanted to the open field for cultivation.

[0015] The bud induction medium in this embodiment is based on MS medium, with 30 g / L sucrose and 10 g / L agar adde...

Embodiment 2

[0019] Take sugarcane axillary buds, wash them with clean water, soak them in 75% alcohol for 55s, then transfer them to 0.1% mercuric chloride solution for 20 minutes, rinse them with sterile distilled water for 4 times; inoculate them into the bud induction medium, adjust the light to 2000LUX, and adjust the temperature At 26°C, cultivate for 13 days; when the new shoots grow to 3 cm, transfer to the medium for strong seedlings, adjust the light to 2500 LUX, and the temperature is 25°C, and cultivate for 15 days; when the shoots reach a height of 2 cm, transfer to the rooting medium. The light was adjusted to 2300 Lux, the temperature was 23°C, and the sugarcane tissue cultured seedlings were obtained by culturing for 12 days until the base of the seedlings grew 1 cm root; the seedlings were hardened and cultivated for 30 days, and finally transplanted to the open field for cultivation.

[0020] The bud induction medium in this embodiment is based on MS medium, with 50 g / L su...

Embodiment 3

[0024] Take sugarcane axillary buds, wash them with clean water, soak them in 75% alcohol for 60s, then soak them in 0.1% mercury chloride solution for 25 minutes, rinse them with sterile distilled water for 5 times; inoculate them into bud induction medium, adjust the light to 2200LUX, and adjust the temperature Cultivate for 10 days at 26°C; when the new shoots grow to 3 cm, transfer to the medium for strong seedlings, adjust the light to 2300 LUX, and the temperature is 24°C, and cultivate for 17 days; when the shoots reach a height of 2 cm, transfer to the rooting medium. The light was adjusted to 2300 Lux, the temperature was 23°C, and the sugarcane tissue cultured seedlings were obtained by culturing for 12 days until 1 cm root grew from the base of the seedlings; the seedlings were hardened and cultivated for 28 days, and finally transplanted to the open field for cultivation.

[0025] The bud induction medium in this embodiment is based on MS medium, with 50 g / L sucrose...

technical field

[0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of sugarcane. Background technique

[0002] Sugarcane is a plant of the Gramineae Saccharum genus and is an important sugar crop in the world. The cultivation of sugarcane is usually based on the axillary buds on the cane stem nodes, and the axillary buds on the stem base remaining in the soil are used as perennial root propagation. The propagation speed of these methods is slow, the genetic traits of the obtained sugarcane seedlings are quite different, and they are susceptible to virus infection during the cultivation process, resulting in serious degeneration of the varieties. Therefore, there is an urgent need to seek a method for high-yield, high-quality, efficient sugarcane propagation. Contents of the invention

[0003] The purpose of the present invention is to provide a method for rapid propagation of sug...

Examples

Embodiment 1

[0014] Take sugarcane axillary buds, wash them with clean water, soak them in 75% alcohol for 45s, then transfer them to 0.05% mercury chloride solution for 30 minutes, rinse them with sterile distilled water for 3 times; inoculate them into the bud induction medium, adjust the light to 1800LUX, and adjust the temperature Cultivate for 15 days at 23°C; when the new shoots grow to 2 cm, transfer to the medium for strong seedlings, adjust the light to 2000 LUX, and cultivate at 23°C for 18 days; when the shoots grow to 1 cm, transfer to the rooting medium. The light was adjusted to 2200 Lux, the temperature was 26°C, and the sugarcane tissue cultured seedlings were obtained by culturing for 12 days until 3 cm roots grew from the base of the seedlings; the seedlings were hardened and cultivated for 25 days, and finally transplanted to the open field for cultivation.

[0015] The bud induction medium in this embodiment is based on MS medium, with 30 g / L sucrose and 10 g / L agar adde...

Embodiment 2

[0019] Take sugarcane axillary buds, wash them with clean water, soak them in 75% alcohol for 55s, then transfer them to 0.1% mercuric chloride solution for 20 minutes, rinse them with sterile distilled water for 4 times; inoculate them into the bud induction medium, adjust the light to 2000LUX, and adjust the temperature At 26°C, cultivate for 13 days; when the new shoots grow to 3 cm, transfer to the medium for strong seedlings, adjust the light to 2500 LUX, and the temperature is 25°C, and cultivate for 15 days; when the shoots reach a height of 2 cm, transfer to the rooting medium. The light was adjusted to 2300 Lux, the temperature was 23°C, and the sugarcane tissue cultured seedlings were obtained by culturing for 12 days until the base of the seedlings grew 1 cm root; the seedlings were hardened and cultivated for 30 days, and finally transplanted to the open field for cultivation.

[0020] The bud induction medium in this embodiment is based on MS medium, with 50 g / L su...

Embodiment 3

[0024] Take sugarcane axillary buds, wash them with clean water, soak them in 75% alcohol for 60s, then soak them in 0.1% mercury chloride solution for 25 minutes, rinse them with sterile distilled water for 5 times; inoculate them into bud induction medium, adjust the light to 2200LUX, and adjust the temperature Cultivate for 10 days at 26°C; when the new shoots grow to 3 cm, transfer to the medium for strong seedlings, adjust the light to 2300 LUX, and the temperature is 24°C, and cultivate for 17 days; when the shoots reach a height of 2 cm, transfer to the rooting medium. The light was adjusted to 2300 Lux, the temperature was 23°C, and the sugarcane tissue cultured seedlings were obtained by culturing for 12 days until 1 cm root grew from the base of the seedlings; the seedlings were hardened and cultivated for 28 days, and finally transplanted to the open field for cultivation.

[0025] The bud induction medium in this embodiment is based on MS medium, with 50 g / L sucrose...