Method for carrying out cornus walteri tissue culture subculture propagation by using lanthanum nitrate

A technology of subculture proliferation and lanthanum nitrate, which is applied in the field of subculture proliferation of T. chinensis tissue culture by using lanthanum nitrate, can solve the problems of low proliferation rate and poor quality of proliferation of T. chinensis tissue culture system, and can meet the requirements of large-scale industrialization. Seedling and industrial development needs, consistent genetic traits, and the effect of shortening the proliferation cycle

Active Publication Date: 2021-04-30
山西省林业和草原科学研究院
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Problems solved by technology

[0005] In order to solve the problems of low proliferation rate and poor proliferation quality of the current hairy tissue culture system, the present invention provides a method for using lanthanum nitrate to carry out subculture proliferation of hairy tissue culture

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  • Method for carrying out cornus walteri tissue culture subculture propagation by using lanthanum nitrate
  • Method for carrying out cornus walteri tissue culture subculture propagation by using lanthanum nitrate
  • Method for carrying out cornus walteri tissue culture subculture propagation by using lanthanum nitrate

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Embodiment Construction

[0020] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than All the embodiments; based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts all belong to the protection scope of the present invention.

[0021] 1. Test material: transfer the axillary buds induced by the stem section of the hairy stem to the proliferation medium for subculture. A single-factor completely randomized design was adopted, with 20 bottles per treatment and repeated 3 times.

[0022] 2. Experimental method:

[0023] A. Add TDZ alone or combine with NAA, IBA, 2,4-D: transfer the induced axillary buds to MS basic medium, and add T...

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Abstract

The invention belongs to the technical field of forest tree tissue culture propagation, and provides a method for performing cornus walteri tissue culture subculture propagation by using lanthanum nitrate. The methodaims to solve the problems of low propagation rate, poor propagation quality and the like of a current cornus walteri tissue culture system. According to the method, MS is adopted as a basic culture medium, tender stems of cornus walteri are taken as explants, axillary bud induction is carried out, then axillary buds are transferred to a proliferation culture medium added with 0.2 mg.L<-1> lanthanum nitrate for subculture proliferation culture, a large number of axillary buds and adventitious buds are generated through induction, and the proliferation rate and the proliferation coefficient reach 91.5% and 18.1 respectively. According to the method, a large number of subculture multiplication materials can be obtained in a short time, the cornus walteri tissue culture subculture multiplication effect is remarkably improved, the subculture bud propagation period is shortened, and therefore the production cost in industrialized seedling raising of cornus walteri tissue culture is reduced.

Description

technical field [0001] The invention belongs to the technical field of tree tissue culture and breeding, and in particular relates to a method for using lanthanum nitrate to carry out subculture and multiplication of hair tree tissue culture. Background technique [0002] Mao 梾 ( Cornus walteri Wanger.), also known as black starling, car beam wood, etc., belongs to the genus of Cornus genus, deciduous trees, concentrated in Shanxi, Shandong, Shaanxi, Henan and other places. The oil content of the fruit is 31.8%-41.3%, and the oil content of the peel is 24.9%-25.7%. It can be used to produce new woody edible oil, health care products, and biodiesel development. It is an important biomass energy tree species. It is resistant to drought and barrenness, has strong adaptability, strong root system, and is not strict on soil. It is a good tree species for greening barren hills and water and soil conservation. It has beautiful tree shape, dense branches and leaves, white flowers ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001Y02P60/21
Inventor 张娜刘劲张春媛李林英王建义杨飞史敏华贺义才杨俊鸾梁爱军马佳琳陈春胡万银高晋东
Owner 山西省林业和草原科学研究院
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