A kind of method and medium for improving the effect of tissue culture subculture of Liquidambar

A technology of subculture multiplication and multiplication cultivation, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of long tissue culture seedling cultivation cycle, low survival rate of old tree spikes, high cost of seedlings, etc., to achieve Shorten the culture cycle of subculture, improve the effect of subculture and improve the utilization rate of materials

Active Publication Date: 2020-07-14
GUANGXI FORESTRY RES INST
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Problems solved by technology

There are mainly two kinds of traditional liquidambar seedling cultivation techniques: one is seed breeding, but the disadvantages of seed breeding seedlings are as follows, one is that the seeds generally germinate every other year, the seedlings have strong seasonality, and it is difficult to preserve the seeds for a long time; the other is the scientifically selected fine varieties Generally, there are few seeds, which are difficult to meet the needs of production and application; third, the forest divisions in the production of seedlings cultivated by selected seeds of superior plants are greatly differentiated, and the forest phases are uneven, which cannot realize large-scale utilization.
The other is cutting breeding, and its disadvantages are as follows. First, the survival rate of old tree spikes is low. To build a cutting nursery for cuttings, a large amount of manual labor is required for fine management, which makes the cost of seedlings higher; The ear-picking mother plant is easy to age, the service life is not long, and it will be eliminated after 3-5 years; the third is that the survival rate of cuttings is affected by various factors such as weather, management, and substrate, and the survival rate is difficult to guarantee
[0004] With the rapid development of tissue culture technology, researchers are trying to use tissue culture technology to carry out large-scale breeding of sweetgum sweetgum, but the following problems exist in the tissue culture of liquid sweetgum: first, during the process of tissue culture subculture bud proliferation , the browning phenomenon is serious, and the bud tips are easy to wither, brown and necrotic; the second is that the cultivation cycle of tissue culture seedlings is long, the main reason is that the time required for the multiplication stage is longer

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  • A kind of method and medium for improving the effect of tissue culture subculture of Liquidambar
  • A kind of method and medium for improving the effect of tissue culture subculture of Liquidambar
  • A kind of method and medium for improving the effect of tissue culture subculture of Liquidambar

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Initial bud induction culture: Select a good single plant with good plant type, stable discoloration, good discoloration effect, and no pests in the forest of Liquidambar sweetgum, carry out pile cutting or circumcision to promote germination in the previous year, collect the year's growth, and grow to Germination strips of 35-40 cm were used as explants, which were inoculated into the first-generation induction medium modified B5+6-BA 2.5mg·L after routine disinfection -1 +NAA 0.5mg·L -1 + sucrose 30g·L -1 + Agar 4.5g·L -1 The initial buds were induced, and the induction rate was 91.2%;

[0033] (2) Proliferation medium: two kinds of medium were prepared for the proliferation of shoots, and the medium for subculture ① was formulated with improved B5+BA 1.0mg·L -1 +NAA 0.1mg·L -1 +PVP 3.0g·L -1 + sucrose 30g·L -1 + Agar 4.5g·L -1 ; The formula of subculture proliferation medium ② adopts improved B5+KT 2.0mg·L -1 +NAA 0.01mg·L -1 +IBA 0.5mg·L -1 +PVP3.0g·L ...

Embodiment 2

[0036] (1) Initial bud induction culture: Select a good single plant with good plant type, stable discoloration, good discoloration effect, and no pests in the forest of Liquidambar sweetgum, carry out pile cutting or circumcision to promote germination in the previous year, collect the year's growth, and grow to Germination strips of 30-35 cm are used as explants, and after routine disinfection, they are inoculated into the first-generation induction medium modified B5+6-BA 2.5 mg·L -1 +NAA 0.5mg·L -1 + sucrose 30g·L -1 + Agar 4.5g·L -1 The initial buds were induced, and the induction rate was 90.7%.

[0037] (2) Proliferation medium: two kinds of medium were prepared for the proliferation of shoots, and the medium for subculture ① was formulated with improved B5+BA 2.0mg·L -1 +NAA 0.18mg·L -1 +PVP 6.0g·L -1 + sucrose 30g·L -1 + Agar 4.5g·L -1 ; Subculture proliferation medium ② formula adopts improved B5+KT 3.0mg·L -1 +NAA 0.01mg·L -1 +IBA 0.5mg·L -1 +PVP 3.0g·L -...

Embodiment 3

[0040] (1) Initial bud induction culture: Select a good single plant with good plant type, stable discoloration, good discoloration effect, and no pests in the forest of Liquidambar sweetgum, carry out pile cutting or circumcision to promote germination in the previous year, collect the year's growth, and grow to Germination strips of 30-40 cm were used as explants, and after routine disinfection, they were inoculated into the first-generation induction medium to improve B5+6-BA 2.5 mg·L -1 +NAA 0.5mg·L -1 + sucrose 30g·L -1 + Agar 4.5g·L -1 The initial buds were induced, and the induction rate was 91.5%.

[0041] (2) Subculture proliferation medium: prepare 2 kinds of subculture proliferation medium for subculture bud proliferation culture, subculture proliferation medium ① formula adopts improved B5+BA2.0mg·L -1 +NAA0.16mg·L -1 +PVP 3.0g·L -1 + sucrose 30g·L -1 + Agar 4.5g·L -1 ; Subculture Proliferation Medium ② formula adopts improved B5+KT 2.5mg L -1 +NAA0.01mg·L ...

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Abstract

The invention discloses a method and a culture medium for improving the subculture proliferation effect of sweetgum sweetgum. In the method, an excellent single plant of sweetgum sweetgum is selected for pile cutting or circumcision to promote germination, and budding strips are collected, and firstly induced and cultured in a specially designed primary generation. The primary shoots were induced by the base, and then cultured in two specially designed subculture mediums using two different cutting methods. Applying the present invention can obtain a large amount of subculture propagation materials in a short period of time, significantly improve the subculture propagation effect of liquidambar tissue culture, shorten the propagation cycle of subculture buds, and reduce the browning rate, thereby reducing the risk in the industrial seedling cultivation of liquidambar tissue culture. Cost of production. In addition, since the explants are collected from a fine single plant of Liquidambar sweetgum, its tissue-cultured seedlings maintain the excellent genetic traits of the female parent, so that the seedlings of Liquidambar sweetgum can be improved and cloned, and have high economic, social and social benefits. Ecological Benefits.

Description

technical field [0001] The invention belongs to the technical field of liquidambar tissue culture and seedling cultivation, and in particular relates to a method and a culture medium for improving the subculture proliferation effect of liquidambar tissue culture. Background technique [0002] Liquidambar formosana Hance is a deciduous tree of the genus Liquidambar in the family Hamamelidaceae. It is widely distributed and is one of the main forest tree species in southern my country. Liquidambar has strong adaptability and fire resistance, and is resistant to drought and barrenness. It is known as the pioneer tree species of barren hills; Excellent urban and forest landscape tree species; liquidambar resin can be used as a spice, and its roots, leaves and fruits are used as medicine, which can dehumidify and activate menstruation and blood circulation, and have important medicinal value; in addition, its bark and leaves contain milk-enriching tannin Liquidambar extract can ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 肖玉菲尹国平刘海龙王勇刘雄盛蒋燚陈博雯姜英黄荣林韦铄星李娟
Owner GUANGXI FORESTRY RES INST
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