Method for improving tissue culture multiplication effect of sweetgum and culture medium

A technology of subculture multiplication and multiplication cultivation, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long tissue culture seedling cultivation cycle, low survival rate of old tree spikes, high cost of seedlings, etc., and achieve shortening Subculture proliferation culture cycle, improve the effect of subculture proliferation, improve the effect of material utilization

Active Publication Date: 2019-04-26
GUANGXI FORESTRY RES INST
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AI Technical Summary

Problems solved by technology

There are mainly two kinds of traditional liquidambar seedling cultivation techniques: one is seed breeding, but the disadvantages of seed breeding seedlings are as follows, one is that the seeds generally germinate every other year, the seedlings have strong seasonality, and it is difficult to preserve the seeds for a long time; the other is the scientifically selected fine varieties Generally, there are few seeds, which are difficult to meet the needs of production and application; third, the forest divisions in the production of seedlings cultivated by selected seeds of superior plants are greatly differentiated, and the forest phases are uneven, which cannot realize large-scale utilization.
The other is cutting breeding, and its disadvantages are as follows. First, the survival rate of old tree spikes is low. To build a cutting nursery for cuttings, a large amount of manual labor is required for fine management, which makes the cost of seedlings higher; The ear-picking mother plant is easy to age, the service life is not long, and it will be eliminated after 3-5 years; the third is that the survival rate of cuttings is affected by various factors such as weather, management, and substrate, and the survival rate is difficult to guarantee
[0004] With the rapid development of tissue culture technology, researchers are trying to use tissue culture technology to carry out large-scale breeding of sweetgum sweetgum, but the following problems exist in the tissue culture of liquid sweetgum: first, during the process of tissue culture subculture bud proliferation , the browning phenomenon is serious, and the bud tips are easy to wither, brown and necrotic; the second is that the cultivation cycle of tissue culture seedlings is long, the main reason is that the time required for the multiplication stage is longer

Method used

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  • Method for improving tissue culture multiplication effect of sweetgum and culture medium
  • Method for improving tissue culture multiplication effect of sweetgum and culture medium
  • Method for improving tissue culture multiplication effect of sweetgum and culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Initial bud induction culture: Select a good single plant with good plant type, stable discoloration, good discoloration effect, and no pests in the forest of Liquidambar sweetgum, carry out pile cutting or circumcision to promote germination in the previous year, collect the year's growth, and grow to Germination strips of 35-40 cm were used as explants, which were inoculated into the first-generation induction medium modified B5+6-BA 2.5mg·L after routine disinfection -1 +NAA 0.5mg·L -1 + sucrose 30g·L -1 + Agar 4.5g·L -1 The initial buds were induced, and the induction rate was 91.2%;

[0033] (2) Proliferation medium: two kinds of medium were prepared for the proliferation of shoots, and the medium for subculture ① was formulated with improved B5+BA 1.0mg·L -1 +NAA 0.1mg·L -1 +PVP 3.0g·L -1 + sucrose 30g·L -1 + Agar 4.5g·L -1 ; The formula of subculture proliferation medium ② adopts improved B5+KT 2.0mg·L -1 +NAA 0.01mg·L -1 +IBA 0.5mg·L -1 +PVP3.0g·L ...

Embodiment 2

[0036] (1) Initial bud induction culture: Select a good single plant with good plant type, stable discoloration, good discoloration effect, and no pests in the forest of Liquidambar sweetgum, carry out pile cutting or circumcision to promote germination in the previous year, collect the year's growth, and grow to Germination strips of 30-35 cm are used as explants, and after routine disinfection, they are inoculated into the first-generation induction medium modified B5+6-BA 2.5 mg·L -1 +NAA 0.5mg·L -1 + sucrose 30g·L -1 + Agar 4.5g·L -1 The initial buds were induced, and the induction rate was 90.7%.

[0037] (2) Proliferation medium: two kinds of medium were prepared for the proliferation of shoots, and the medium for subculture ① was formulated with improved B5+BA 2.0mg·L -1 +NAA 0.18mg·L -1 +PVP 6.0g·L -1 + sucrose 30g·L -1 + Agar 4.5g·L -1 ; Subculture proliferation medium ② formula adopts improved B5+KT 3.0mg·L -1 +NAA 0.01mg·L -1 +IBA 0.5mg·L -1 +PVP 3.0g·L -...

Embodiment 3

[0040] (1) Initial bud induction culture: Select a good single plant with good plant type, stable discoloration, good discoloration effect, and no pests in the forest of Liquidambar sweetgum, carry out pile cutting or circumcision to promote germination in the previous year, collect the year's growth, and grow to Germination strips of 30-40 cm were used as explants, and after routine disinfection, they were inoculated into the first-generation induction medium to improve B5+6-BA 2.5 mg·L -1 +NAA 0.5mg·L -1 + sucrose 30g·L -1 + Agar 4.5g·L -1 The initial buds were induced, and the induction rate was 91.5%.

[0041] (2) Subculture proliferation medium: prepare 2 kinds of subculture proliferation medium for subculture bud proliferation culture, subculture proliferation medium ① formula adopts improved B5+BA2.0mg·L -1 +NAA0.16mg·L -1 +PVP 3.0g·L -1 + sucrose 30g·L -1 + Agar 4.5g·L -1 ; Subculture Proliferation Medium ② formula adopts improved B5+KT 2.5mg L -1 +NAA0.01mg·L ...

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Abstract

The invention discloses a method for improving a tissue culture multiplication effect of sweetgum and a culture medium. The method comprises the following steps: selecting superior individuals of sweetgum to stump or perform girdling for promoting germination, collecting budding branches, and inducing by a specifically designed primary induction medium to produce initial buds; and inoculating intotwo specifically designed multiplication media to culture by adopting two different cut modes. According to the method disclosed by the invention, lots of multiplication materials can be obtained ina short time, the tissue culture multiplication effect of the sweetgum is obviously improved, the bud multiplication period is shortened, and the browning rate is reduced, so that the production costin tissue culture industrialized seedling raising of the sweetgum is reduced. In addition, since explants adopt the superior individuals of sweetgum, the tissue culture seedlings maintain excellent inheritable characters of female parents, the sweetgum seedlings achieve improved breeds and cloning, and the method has high economic benefits, social benefits and ecological benefits.

Description

technical field [0001] The invention belongs to the technical field of liquidambar tissue culture and seedling cultivation, and in particular relates to a method and a culture medium for improving the subculture proliferation effect of liquidambar tissue culture. Background technique [0002] Liquidambar formosana Hance is a deciduous tree of the genus Liquidambar in the family Hamamelidaceae. It is widely distributed and is one of the main forest tree species in southern my country. Liquidambar has strong adaptability and fire resistance, and is resistant to drought and barrenness. It is known as the pioneer tree species of barren hills; Excellent urban and forest landscape tree species; liquidambar resin can be used as a spice, and its roots, leaves and fruits are used as medicine, which can dehumidify and activate menstruation and blood circulation, and have important medicinal value; in addition, its bark and leaves contain milk-enriching tannin Liquidambar extract can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 肖玉菲尹国平刘海龙王勇刘雄盛蒋燚陈博雯姜英黄荣林韦铄星李娟
Owner GUANGXI FORESTRY RES INST
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