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Tissue culture method of begonia

A technology of tissue culture and culture medium, which is applied in the field of tissue culture of begonia leaves, can solve the problems of seasonal limitation of reproduction and low reproduction efficiency, and achieves the effects of short reproduction time, improved reproduction efficiency and good economic benefits.

Active Publication Date: 2018-05-25
四川立德种苗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, leaf-viewing begonias are mainly propagated by leaf cuttings, but the propagation is limited by the season and the reproduction efficiency is low, which is far from meeting the domestic demand for seedlings

Method used

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  • Tissue culture method of begonia
  • Tissue culture method of begonia
  • Tissue culture method of begonia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The tissue culture method of the foliage begonia of the present invention comprises the following steps:

[0047] Pretreatment: take the leaf-viewing begonia and cut them into 1cm*1cm squares through the young leaves, sterilize them with mercury chloride for 2-3 minutes, wash them with sterile water for 3-5 times, and obtain the explants after sterilization; The leaves of Begonia foliage are the new leaves 30 days after the leaves of Guanye Qiuhai are inserted through the young leaves;

[0048] Primary culture: inoculate the sterilized explants on the first culture medium for primary culture to obtain the first generation clustered shoots; the composition of the first culture medium is: MS basic medium supplemented with 1.5mg / L KT , 0.2mg / L NAA, 0.1mg / L 2,4-D, 30g / L sucrose, 8g / L agar; figure 1 shown

[0049] Induction culture: inoculate the first-generation clustered shoots into the second medium for induction culture to obtain rooted shoots; the second medium is 1 / 2MS...

Embodiment 2

[0055] The difference between the present invention and Example 1 is only that: the composition of the first medium is: MS basic medium supplemented with 0.2mg / L KT, 0.05mg / L NAA, 0.02mg / L 2,4-D, 10g / L sucrose, 6g / L agar.

[0056] In this embodiment, during the primary culture process, the primary culture time is 30-35 days. During the induction culture process, it takes 30-35 days for the induction culture to obtain rooted seedlings; the average root number of each rooted seedling is 10-14, the root length is 6-10 cm, and the plant height of the rooted seedlings is 2-3 cm. During the multiplication and cultivation process, the plant height of the obtained strong seedlings is 3-6 cm, the multiplication and cultivation time is 30-35 days, and the multiplication coefficient is 8.

Embodiment 3

[0058] The only difference between the present invention and Example 1 is that the composition of the first medium is: MS basic medium supplemented with 0.5mg / L KT, 0.1mg / L NAA, 0.1mg / L 2,4-D, 20g / L sucrose, 7g / L agar.

[0059] In this embodiment, during the primary culture process, the primary culture time is 20-25 days. During the induction culture process, it takes 20-25 days for the induction culture to obtain rooted seedlings; the average root number of each rooted seedling is 14-18 roots, the root length is 8-12 cm, and the plant height of the rooted seedlings is 3-4 cm. During the multiplication culture process, the plant height of the strong seedlings obtained is 4-7 cm, the multiplication culture time is 25-30 days, and the multiplication coefficient is 10.

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Abstract

The invention discloses a tissue culture method of begonia. The tissue culture method comprises the following steps: pretreatment: taking young leaves of the begonia for cutting, sterilizing and cleaning to obtain a sterilized explant; primary culture: inoculating the sterilized explant into a first culture medium for carrying out the primary culture to obtain primary cluster buds; induction culture: inoculating the primary cluster buds into a second culture medium for carrying out induction culture to obtain rooting seedlings; multiplication culture: cutting leaf blades of the rooting seedlings as female parent, then inoculating the leaf blades into the first culture medium for inducing culture to obtain subcultured cluster buds; inoculating the subcultured cluster buds into a second culture medium for culturing to obtain strong seedlings; acclimatization and transplant: carrying out acclimatization on the strong seedlings and transplanting to obtain domesticated seedlings of the begonia. The tissue culture method has the advantages of capability of improving the survival rate of the begonia hardened seedlings, short culture period, high propagation coefficient, simple tissue culture flow and no restriction from season.

Description

technical field [0001] The invention relates to the technical field of plant asexual reproduction, in particular to a tissue culture method for leaf-watching begonias. Background technique [0002] Leaf-viewing begonia (Begonia) is native to tropical or subtropical regions. It is a perennial evergreen herb with various varieties and characteristics. Leaf-viewing begonia is popular as a foliage plant in recent years because of its beautiful leaf shape and gorgeous leaf color, and it is used for indoor plum blossoms. At present, leaf-viewing begonias are mainly propagated by leaf cuttings, but the propagation is limited by seasons and the propagation efficiency is low, which is far from meeting the domestic demand for seedlings. Contents of the invention [0003] In view of this, the present application provides a tissue culture method of begonia foliage; the tissue culture method improves the survival rate of hardening begonia foliage, has a short cultivation cycle, high r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 高尚尧森马建华朱晓菲王小辉沈香兰
Owner 四川立德种苗科技有限公司
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