One-step tissue culture and rapid propagation method of grapes
A technology for tissue culture and rapid propagation of grapes, applied in the field of viticulture, can solve the problems of complex operation steps and increased difficulty, and achieve the effects of improving cultivation efficiency, reducing operation difficulty, and shortening the reproduction cycle
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Embodiment 1
[0012] Grape one-step tissue culture rapid propagation method, it comprises the following steps:
[0013] S1. Sterilize the explants, collect the semi-lignified grape stems of the year, cut them into sections with buds, wash them with running water, and then sterilize them on an ultra-clean bench. The sterilization method is a combination of alcohol and mercuric chloride , first sterilize with alcohol for 45s, and then sterilize with mercuric chloride for 4min;
[0014] S2, inoculation and propagation, inoculate the sprouted stem section after sterilization on the growth medium, such as figure 1 As shown, the medium is MS+NAA0.2mg / L+IAA0.1mg / L, and the inoculated culture material is placed in a culture with a light intensity of 3000LX, a light time of 16h / d, and a temperature of 25°C. Chamber culture, the reproduction cycle is one week, to realize the simultaneous multiplication and rooting of the culture materials, and after one week, the budded stem segments grow into compl...
Embodiment 2
[0016] Grape one-step tissue culture rapid propagation method, it comprises the following steps:
[0017] S1. Sterilize the explants. Collect semi-lignified grape stems that were born in the same year, cut them into 2-3cm single-bud stem segments, wash them with running water, and then sterilize them on an ultra-clean bench. The sterilization method uses alcohol plus mercury chloride. Combined sterilization method, first rinse with 70% alcohol by volume for 30 seconds, then rinse with sterile water for 3 times, soak in 0.1% mercury solution for disinfection for 5 minutes, and rinse with sterile water for 3 to 5 minutes Second-rate,
[0018] S2, inoculation and propagation, inoculate the sprouted stem section after sterilization on the growth medium, such as figure 1 As shown, the medium is MS+NAA0.2mg / L+IAA0.1mg / L, pH 5.8-6.0, and the inoculated culture material is placed in a light intensity of 3000LX, a light time of 16h / d, and a temperature of It is cultivated in a cultur...
Embodiment 3
[0020] Grape one-step tissue culture rapid propagation method, it comprises the following steps:
[0021] S1. Sterilize the explants. Collect semi-lignified grape stems that were born in the same year, cut them into 2-3cm single-bud stem segments, wash them with running water, and then sterilize them on an ultra-clean bench. The sterilization method uses alcohol plus mercury chloride. Combined sterilization method, first rinse with 70% alcohol by volume for 60 seconds, then rinse with sterile water for 3 times, soak in 0.1% mercury solution for disinfection for 3 minutes, rinse with sterile water for 3 times,
[0022] S2, inoculation and propagation, inoculate the sprouted stem section after sterilization on the growth medium, such as figure 1 As shown, the medium is MS+NAA0.2mg / L+IAA0.1mg / L, pH 5.8, and the inoculated culture material is placed in a light intensity of 3000LX, a light time of 16h / d, and a temperature of 26 Cultivate in a culture room at ℃, and the propagation...
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