A kind of method for plant regeneration of camphor variety Yongjin

Abstract

The invention discloses a method for regenerating a camphor variety yongjin (Chinese character) plant. The method comprises the following steps: (1) collecting a new twig of camphor variety 'yongjin', which grows in current year, removing leaves after the new twig is taken back to a laboratory; cutting into stems with 1-2 auxiliary buds, asepticizing the stems, then inoculating stems into MS culture medium containing 6-benzyl amino adenine and indolebutyric acid to induce formation of the auxiliary buds; (2) inoculating the 'yongjin' auxiliary buds which grow well into the MS culture medium containing 6-benzyl amino adenine and indolebutyric acid together with the stems, so as to carry out subculture and multiplication cultivation; (3) cutting proliferated cluster buds which are small, and grow well, with the mean length being about 2.0cm by using a dissecting knife, and then inoculating the cut cluster buds into 1 / 2 MS culture medium containing indolebutyric acid so as to induce formation of adventitious roots. By adopting the method disclosed by the invention, the phenomena of difficult auxiliary buds induction and growth, black death of subcultured and proliferated new leaves, dropping, and weak growth are overcome, and an asepsis material is sub-cultured for a long period of time. Thus, great proliferation is achieved, the induced cluster buds are vigorous in growth, and the inheritable characters of the induced cluster buds are same as those of a female parent.

Description

technical field [0001] The invention belongs to the field of tissue culture, and in particular relates to a method for plant regeneration of the camphor variety Yongjin. Background technique [0002] Camphor tree (Cinnamomumcaphora), also known as camphor, is a subtropical evergreen broad-leaved tree of the genus Lauraceae. It grows rapidly, has luxuriant branches and leaves, is evergreen in all seasons, and has beautiful leaf shape and strong fragrance. It is not only a precious aromatic oil tree species but also an important landscape and greening tree species in cities. Cinnamomum camphora new variety Yongjin (Cinnamomumcamphora'Yongjin', National Plant Variety Right No. 2009009) is a variant of camphor seed, with bright red branches, new leaves and flowers in golden yellow, yellow peel, and branches, leaves, The color of the peel has seasonal changes. As a tall tree with colorful leaves, it has very high potential and value for garden viewing and promotion and applicati...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the above-mentioned prior art, the present invention mainly fills in the gap in the existing Cinnamomum camphora new variety Yongjin tissue culture technology, aiming at the induction and growth difficulties of axillary buds in the regeneration process of Yongjin plants with stems as explants, axillary buds In the process of subculture and multiplication, new leaves fall off and black dead, clustered shoots are difficult to take root, etc., the culture conditions suitable for each stage of Yongjin tissue culture and rapid propagation are studied, aiming to provide effective help for the realization of Yongjin's rapid propagation

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