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Method for carrying out bulb induction and plant regeneration of wide-leaf albuca namaquensis by flower buds

A spring grass and bulb technology is applied in the field of spring grass bulb induction and plant regeneration, which can solve the problems of inducing spring grass bulbs and other problems, and achieves overcoming the scarcity of female parents, simplifying tissue culture operation procedures, and overcoming the slowness of conventional propagation methods. Effect

Active Publication Date: 2015-04-08
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

However, there is no report on establishment of spring grass tissue culture before this, and there is no successful example of inducing spring grass bulb and plant regeneration through flower buds

Method used

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Embodiment

[0040] The invention provides a method for inducing spring grass bulbs and plant regeneration through flower buds, the steps of which are:

[0041] 1), the preparation of culture medium

[0042] (1) Basic medium: MS medium, wherein sucrose 30g / L, agar 7.5g / L, pH=5.8;

[0043] (2) Induction medium: MS+6-BA 4.0mg / L+NAA 0.5mg / L;

[0044] (3) Proliferation medium: MS+6-BA 0.5mg / L+NAA 0.05mg / L;

[0045] (4) Strong seedling rooting medium: MS+6-BA 0.01mg / L+NAA 0.1mg / L.

[0046] 2), selection and sterilization of explants

[0047] Take the young inflorescences of Spring Grass, remove the outer covering of the closed flower buds on the inflorescences, cut off the flower buds and some scapes one by one, soak them in saturated washing powder solution for 60 minutes, then rinse them with tap water, and then wash them in volume Soak in 70% alcohol and 1% sodium hypochlorite solution for 1 min and 6 min respectively, and finally rinse with sterile water for 5 times; 3), induction and p...

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Abstract

The invention discloses a method for carrying out bulb induction and plant regeneration of wide-leaf albuca namaquensis by flower buds. The method comprises the following specific steps: (1) preparation of a culture medium including a basic culture medium and components of the culture medium at all stages of tissue culture; (2) selection and disinfection of explants; (3) induction and multiplication of bulbs; (4) rooting of strong seedlings; and (5) transplantation. The method disclosed by the invention has the beneficial effects that the direct induction and the plant regeneration of the bulbs are carried out on flower buds of the wide-leaf albuca namaquensis by utilizing a plant-tissue culture technology, and the procedure of tissue-culture operation is simplified, so that a great quantity of high-quality strong seedlings with consistent genetic characters can be obtained in short time, simultaneously, the defects of female-parent scarcity and slowness of conventional multiplication methods are overcome, and the method has the positive significance for both protecting germplasm resources of the albuca namaquensis and industrializing seedling production.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a method for inducing spring grass bulbs and plant regeneration through flower buds. Background technique [0002] Albuca concordiana (Albuca concordiana) is a perennial ornamental succulent plant of the family Hyacinthaceae. It is native to South Africa. Its ribbon-shaped leaves are twisted and circled, and its spherical bulbs are simple and unique. Its flowers are fresh and elegant. It is a precious spring grass variety. Under natural conditions, spring grass grows slowly and has a low reproductive rate; at the same time, the International Organization for Succulent Plant Study (The International Organization for Succulent Plant Study) has been in the 1970s in order to protect wild succulents from predatory collection or even extinction. The "Cultivation Knowledge of Succulent Plants and Code of Conduct for Collectors" was formulated, which greatly restricted the introduction o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王燕汪一婷牟豪杰吕永平陈志陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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