Fast reproduction of European wild black cherry tissue

A technology for tissue culture rapid propagation and black cherry, which is applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve problems such as inability to achieve large-scale industrial production, yellowing, and difficulty in woody plants, and achieve good economical results. Socio-ecological benefits, increased market share, consistent effects of genetic traits

Inactive Publication Date: 2009-10-28
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different plants have different degrees of difficulty in propagating seedlings using plant tissue culture methods, especially woody plants are more difficult.
In the preliminary tissue culture test of European black cherry wood, some problems that often occur in tissue culture of woody plants also appeared, such as high pollution rate of explant inoculation, easy vitrification and yellowing of tissue culture seedlings, and tissue culture seedlings Problems such as difficulty in rooting and low survival rate of transplanting make it impossible to realize large-scale factory production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: this European black cherry wood tissue culture rapid propagation method, concrete steps are as follows:

[0031] 1), selection and sterilization of explants: cut semi-lignified shoots of potted wild black cherry seedlings, cut off the terminal buds and stems with axillary buds, and soak the explants in 75% alcohol for 0.5-10 minutes , sterilized with 2% sodium hypochlorite aqueous solution by volume for 10-15 minutes, and then rinsed with sterile water for 3-5 times. After surface sterilization, it was used as explants for tissue culture.

[0032] 2), induction culture: under aseptic conditions, after the explant terminal bud and the stem section with axillary buds after surface sterilization are removed, the terminal buds and the buds of the stem section with axillary buds are cut off. Inoculate upward on the induction medium of MS+BA0.25~1.0mg / L+NAA0.05~0.5mg / L to induce terminal buds and axillary buds to sprout, and then differentiate into cluster buds...

Embodiment 2

[0037] Embodiment 2: this European black cherry wood tissue culture rapid propagation method, concrete steps are as follows:

[0038] 1), selection and sterilization of explants: cut semi-lignified shoots of potted wild black cherry seedlings, cut off the terminal buds and stems with axillary buds, and soak the explants in 75% alcohol for 0.5-10 minutes , sterilized with 2% sodium hypochlorite aqueous solution by volume for 10-15 minutes, and then rinsed with sterile water for 3-5 times. The surface was sterilized and used as explants for tissue culture.

[0039] 2), induction culture: under aseptic conditions, after the explant terminal bud and the stem section with axillary buds after surface sterilization are removed, the terminal buds and the buds of the stem section with axillary buds are cut off. Inoculate upward on the induction medium of MS+BA0.75mg / L+NAA0.1mg / L to induce the germination of terminal buds and axillary buds, and then differentiate into cluster buds.

...

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Abstract

The present invention relates to a kind of method for rapid propagation of European black cherry wood tissue culture, which is carried out according to the following steps: 1) selection and sterilization of explants; 2) induction culture: the terminal buds of explants after surface sterilization After the browning part of one or two ends of the stem section with axillary buds is removed, the terminal buds and the buds of the stem section with axillary buds are upwardly inoculated on the induction medium to induce the terminal buds and axillary buds to germinate, and then differentiate cluster buds; 3) Proliferation Cultivation: Cut the cluster buds into single plants and insert them into the proliferation medium for proliferation and culture to induce the cluster buds; 4) rooting culture: inoculate into rooting medium 1 for cultivation, then transfer to rooting medium 2 for cultivation, and induce its rooting 5) Domestication and transplanting of tissue-cultured seedlings: First, domesticate the tissue-cultured seedlings, and then transplant into the mixed matrix of peat, perlite and vermiculite. The beneficial effects of the present invention are: the monthly multiplication rate can reach 3 times, the tissue culture seedlings have no vitrification and yellowing, the rooting rate reaches 100%, the tissue culture seedlings grow robustly, the leaf color is dark green, and the transplanting survival rate can reach 95%. .

Description

technical field [0001] The invention relates to a woody plant tissue culture technology, mainly a method for tissue culture and rapid propagation of European black cherry wood. Background technique [0002] European wild cherry wood (Wild Cherry, PrunusAvium wildstar) is a cultivar cultivated from wild black cherry wood found in the UK. This tree species is the only tree species in the genus Prunus that has commercial timber value. This tree species is a tall tree with a maximum height of 39 meters and a maximum diameter of 2.5 meters. The trunk is straight and round, with few main branches. The main trunk is 3 to 6 meters above the ground without branches. The wood is of high quality, light and solid, with straight and fine grain, and the color is light red to light reddish brown. It is a high-quality material for high-end furniture and plywood veneer. The economic value is very high. The price of original floor materials is 1500-2000 per cubic meter Dollar. It is also wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 徐刚汪一婷吕永平牟豪杰陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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