Fast reproduction of European wild black cherry tissue

Abstract

The present invention relates to a kind of method for rapid propagation of European black cherry wood tissue culture, which is carried out according to the following steps: 1) selection and sterilization of explants; 2) induction culture: the terminal buds of explants after surface sterilization After the browning part of one or two ends of the stem section with axillary buds is removed, the terminal buds and the buds of the stem section with axillary buds are upwardly inoculated on the induction medium to induce the terminal buds and axillary buds to germinate, and then differentiate cluster buds; 3) Proliferation Cultivation: Cut the cluster buds into single plants and insert them into the proliferation medium for proliferation and culture to induce the cluster buds; 4) rooting culture: inoculate into rooting medium 1 for cultivation, then transfer to rooting medium 2 for cultivation, and induce its rooting 5) Domestication and transplanting of tissue-cultured seedlings: First, domesticate the tissue-cultured seedlings, and then transplant into the mixed matrix of peat, perlite and vermiculite. The beneficial effects of the present invention are: the monthly multiplication rate can reach 3 times, the tissue culture seedlings have no vitrification and yellowing, the rooting rate reaches 100%, the tissue culture seedlings grow robustly, the leaf color is dark green, and the transplanting survival rate can reach 95%. .

Description

technical field [0001] The invention relates to a woody plant tissue culture technology, mainly a method for tissue culture and rapid propagation of European black cherry wood. Background technique [0002] European wild cherry wood (Wild Cherry, PrunusAvium wildstar) is a cultivar cultivated from wild black cherry wood found in the UK. This tree species is the only tree species in the genus Prunus that has commercial timber value. This tree species is a tall tree with a maximum height of 39 meters and a maximum diameter of 2.5 meters. The trunk is straight and round, with few main branches. The main trunk is 3 to 6 meters above the ground without branches. The wood is of high quality, light and solid, with straight and fine grain, and the color is light red to light reddish brown. It is a high-quality material for high-end furniture and plywood veneer. The economic value is very high. The price of original floor materials is 1500-2000 per cubic meter Dollar. It is also wi...

AI Technical Summary

Problems solved by technology

However, different plants have different degrees of difficulty in propagating seedlings using plant tissue culture methods, especially woody plants are more difficult.

In the preliminary tissue culture test of European black cherry wood, some problems that often occur in tissue culture of woody plants also appeared, such as high pollution rate of explant inoculation, easy vitrification and yellowing of tissue culture seedlings, and tissue culture seedlings Problems such as difficulty in rooting and low survival rate of transplanting make it impossible to realize large-scale factory production