Fast reproduction of European wild black cherry tissue

A technology for tissue culture rapid propagation and black cherry, which is applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve problems such as inability to achieve large-scale industrial production, yellowing, and difficulty in woody plants, and achieve good economical results. Socio-ecological benefits, increased market share, consistent effects of genetic traits

Inactive Publication Date: 2007-09-05
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different plants have different degrees of difficulty in propagating seedlings using plant tissue culture methods, especially woody plants are more difficult.
In the preliminary tissue culture test of European black cherry wood, some problems that often occur in tissue culture of woody plants also appeared, such as high pollution rate of explant inoculation, easy vitrification and yellowing of tissue culture seedlings, and tissue culture seedlings Problems such as difficulty in rooting and low survival rate of transplanting make it impossible to realize large-scale factory production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: This method of tissue culture and rapid propagation of wild cherry, the specific steps are as follows:

[0031] 1) Selection and sterilization of explants: Cut the semi-lignified twigs of the potted wild cherry seedlings, cut the apical buds and the stems with axillary buds, and soak the explants with 75% alcohol for 0.5~1.0 Min, sterilize with a 2% sodium hypochlorite aqueous solution by volume for 10-15 minutes, and then rinse with sterile water 3 to 5 times. After surface sterilization, it can be used as an explant for tissue culture.

[0032] 2) Induction culture: under aseptic conditions, the apical buds and the stems with axillary buds of the explants are cut off from the browned part at one or both ends of the apical buds and the stems with axillary buds. Upward inoculation on the induction medium of MS+BA0.25~1.0mg / L+NAA0.05~0.5mg / L to induce apical and axillary buds to germinate, and then differentiate into clump buds.

[0033] 3) Proliferation and cultu...

Embodiment 2

[0037] Example 2: This method for tissue culture and rapid propagation of wild cherry, the specific steps are as follows:

[0038] 1) Selection and sterilization of explants: Cut the semi-lignified twigs of the potted wild cherry seedlings, cut the apical buds and the stems with axillary buds, and soak the explants with 75% alcohol for 0.5~1.0 Min, sterilize with a 2% sodium hypochlorite aqueous solution by volume for 10-15 minutes, and then rinse with sterile water 3 to 5 times. After surface sterilization, it can be used as an explant for tissue culture.

[0039] 2) Induction culture: under aseptic conditions, the apical buds and the stems with axillary buds of the explants are cut off from the browned part at one or both ends of the apical buds and the stems with axillary buds. Upward inoculation on the induction medium of MS+BA0.75mg / L+NAA0.1mg / L to induce apical and axillary buds to germinate, and then differentiate into cluster buds.

[0040] 3) Proliferation and culture: Th...

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PUM

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Abstract

A tissue culture method for fast reproduction of wild European cherry includes such steps as choosing explant, sterilizing it, removing two brocon ends, inoculating it in the inductive culture medium, inductive culture to induce terminal bud and axillary bud, differentiating rosette buds, cutting the rosette buds to obtain single bud, reproductive culture in the reproducing culture medium, rooting culture in the culture medium A and then in culture medium B to obtain tissue cultured seedlings, naturalizing, and transplanting them in the matrix prepared from peat, pearlite and vermiculite.

Description

Technical field [0001] The present invention relates to woody plant tissue culture technology, and is mainly a method for tissue culture and rapid propagation of wild cherry. Background technique [0002] Wild Cherry (Prunus Avium wildstar) is a cultivated species cultivated from wild black cherry found in the UK. This species is the only species in the genus Cherry with commercial timber value. The tree species is a tall tree with a height of up to 39 meters and a maximum diameter at breast height of 2.5 meters. The trunk is straight and perfect, with few main branches, and the main trunk is 3-6 meters above the ground without branches. The wood is of high quality, light, solid, straight and dense in texture, light red to light reddish brown in color. It is a high-quality material for high-end furniture and plywood veneer. It has a high economic value. The original board price is 1500-2000 per cubic meter. Dollar. It is also widely used in aircraft, ships, military furniture, ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 徐刚汪一婷吕永平牟豪杰陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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