Tissue cultivation quick breeding method of kangarno paws

Abstract

The invention relates to a tissue culture and rapid propagation method for anigozanthos and the method is divided into the following steps: (1) preparation of culture medium, including various components of basic culture medium and culture medium in each stage of the tissue culture; (2) selection of explants; (3) pretreatment of explants; (4) disinfection treatment of explants; (5) induction cultivation which is to introduce the induction culture medium for induction cultivation to induce seedling; (6) multiplication culture, the seedling is transferred to multiplication culture medium for multiplication culture of multiple bud; (7) seedling strengthening culture; (8) rooting culture, the multiple bud is cut into single plant which is to be planted in rooting culture medium; (9) cultivation and transplant of tissue culture seedling. The invention has the beneficial effects that by adopting induction culture medium, multiplication culture medium, seedling strengthening culture medium and rooting culture medium and rapid propagation method for anigozanthos, the induction rate of bud is above 90 percent, rooting rate is of 100% and the survival rate of transplanted seedling is above 90 percent, and effectively prevents browning of explants and tissue culture seedling and promotes the differentiation and growth of tissue culture seedling.

Description

technical field

[0001] The invention relates to the technical field of plant tissue culture, and mainly relates to a method for tissue culture and rapid propagation of kangaroo flowers. Background technique

[0002] Kangaroo flower (Anigozanthos flavidus) is a temperate perennial herb in the family Haemodoaceae, endemic to Australia. Its plant height is mostly 1 meter, and the flower shape is unique. The whole flower is in a wonderful claw shape. Bright green petals bloom on the red (yellow) flower stem, which is as beautiful as velvet. It is an excellent cut flower, and can also be planted in gardens or Potted plants are new types of flowers that have only been introduced in recent years and have a good market prospect. However, due to limited provenance, short seed life, low ramet reproduction coefficient, and easy to be affected by seasons and other factors, it is not conducive to large-scale propagation of seedlings.

[0003] Zhejiang Academy of Agricultural Sciences i...