Tissue cultivation quick breeding method of kangarno paws

A tissue culture rapid propagation and kangaroo technology is applied in the field of plant tissue culture, which can solve the problems of low survival rate of transplanting, easy browning of tissue culture seedlings, and different degrees of difficulty in propagating seedlings, so as to overcome the low reproduction coefficient and promote the Differentiation and Growth, Consistent Effects of Genetic Traits

Inactive Publication Date: 2008-01-30
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different plants have different degrees of difficulty in propagating seedlings by using plant tissue culture methods. In the early stage tissue culture test of kangaroo flower, the contamination rate of explant inoculation was high, the explant was easy to brown, and the tissue culture seedling was easy to grow. Problems such as browning and low survival rate of transplanting make it impossible to realize large-scale factory production

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: this Kangaroo flower tissue culture rapid propagation method in the present embodiment 1 is carried out as follows:

[0034] 1), the preparation of the medium, including the basic medium and the composition of the medium suitable for different tissue culture stages, the contents of the basic medium and the medium additives of each stage are:

[0035] (1) Basic medium: MS or 3 / 4MS or 1 / 2MS, in which sucrose or white sugar is 20-30g / L, agar is 7-9g / L, pH5.8;

[0036] (2) Induction medium: MS+6-BA2~5mg / L+IAA0.1~0.5mg / L+ activated carbon 1~3g / L

[0037] (3) Proliferation medium: MS+6-BAl~3mg / L+IAA0.1~0.3mg / L+activated carbon 1~3g / L

[0038] (4) Strong seedling medium: 3 / 4MS+6-BA0.5~2mg / L+IAA0.05~0.2mg / L+activated carbon 1~3g / L

[0039] (5) Rooting medium: 1 / 2MS+NAA0.1~1.0mg / L+ activated carbon 1~3g / L

[0040] 2), the selection of explants: select the kangaroo flower plants with good growth and flowering traits and typical traits of breeding varieties as the...

Embodiment 2

[0050] Embodiment 2: this Kangaroo flower tissue culture rapid propagation method in present embodiment 2, carry out as follows:

[0051] 1), the preparation of the medium, including the basic medium and the composition of the medium suitable for different tissue culture stages, the contents of the basic medium and the medium additives of each stage are:

[0052] (1) Basic medium: MS or 3 / 4MS or 1 / 2MS, in which sucrose or white sugar is 20-30g / L, agar is 7-9g / L, pH5.8;

[0053] (2) Induction medium: MS+6-BA3mg / L+IAA0.1mg / L+activated carbon 3g / L

[0054] (3) Proliferation medium: MS+6-BA2mg / L+IAA0.1mg / L+activated carbon 1g / L

[0055] (4) Strong seedling medium: 3 / 4MS+6-BA1.0mg / L+IAA0.1mg / L+activated carbon 1g / L

[0056] (5) Rooting medium: 1 / 2MS+NAA 0.3mg / L+activated carbon 1g / L.

[0057] 2), the selection of explants: select the kangaroo flower plants with good growth and flowering traits and typical traits of breeding varieties as the source of explants. In the season of ...

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Abstract

The invention relates to a tissue culture and rapid propagation method for anigozanthos and the method is divided into the following steps: (1) preparation of culture medium, including various components of basic culture medium and culture medium in each stage of the tissue culture; (2) selection of explants; (3) pretreatment of explants; (4) disinfection treatment of explants; (5) induction cultivation which is to introduce the induction culture medium for induction cultivation to induce seedling; (6) multiplication culture, the seedling is transferred to multiplication culture medium for multiplication culture of multiple bud; (7) seedling strengthening culture; (8) rooting culture, the multiple bud is cut into single plant which is to be planted in rooting culture medium; (9) cultivation and transplant of tissue culture seedling. The invention has the beneficial effects that by adopting induction culture medium, multiplication culture medium, seedling strengthening culture medium and rooting culture medium and rapid propagation method for anigozanthos, the induction rate of bud is above 90 percent, rooting rate is of 100% and the survival rate of transplanted seedling is above 90 percent, and effectively prevents browning of explants and tissue culture seedling and promotes the differentiation and growth of tissue culture seedling.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and mainly relates to a method for tissue culture and rapid propagation of kangaroo flowers. Background technique [0002] Kangaroo flower (Anigozanthos flavidus) is a temperate perennial herb in the family Haemodoaceae, endemic to Australia. Its plant height is mostly 1 meter, and the flower shape is unique. The whole flower is in a wonderful claw shape. Bright green petals bloom on the red (yellow) flower stem, which is as beautiful as velvet. It is an excellent cut flower, and can also be planted in gardens or Potted plants are new types of flowers that have only been introduced in recent years and have a good market prospect. However, due to limited provenance, short seed life, low ramet reproduction coefficient, and easy to be affected by seasons and other factors, it is not conducive to large-scale propagation of seedlings. [0003] Zhejiang Academy of Agricultural Sciences i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 徐刚汪一婷牟豪杰吕永平陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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