Method for regenerating camphor variety yongjin (Chinese character) plant

Abstract

The invention discloses a method for regenerating a camphor variety yongjin (Chinese character) plant. The method comprises the following steps: (1) collecting a new twig of camphor variety 'yongjin', which grows in current year, removing leaves after the new twig is taken back to a laboratory; cutting into stems with 1-2 auxiliary buds, asepticizing the stems, then inoculating stems into MS culture medium containing 6-benzyl amino adenine and indolebutyric acid to induce formation of the auxiliary buds; (2) inoculating the 'yongjin' auxiliary buds which grow well into the MS culture medium containing 6-benzyl amino adenine and indolebutyric acid together with the stems, so as to carry out subculture and multiplication cultivation; (3) cutting proliferated cluster buds which are small, and grow well, with the mean length being about 2.0cm by using a dissecting knife, and then inoculating the cut cluster buds into 1 / 2 MS culture medium containing indolebutyric acid so as to induce formation of adventitious roots. By adopting the method disclosed by the invention, the phenomena of difficult auxiliary buds induction and growth, black death of subcultured and proliferated new leaves, dropping, and weak growth are overcome, and an asepsis material is sub-cultured for a long period of time. Thus, great proliferation is achieved, the induced cluster buds are vigorous in growth, and the inheritable characters of the induced cluster buds are same as those of a female parent.

Description

technical field [0001] The invention belongs to the field of tissue culture, and in particular relates to a plant regeneration method of a camphor variety 'Yongjin'. Background technique [0002] Camphor tree (Cinnamomum caphora), also known as camphor, is a subtropical evergreen broad-leaved tree belonging to the family Lauraceae. It grows rapidly, has luxuriant branches and leaves, is evergreen in all seasons, and has beautiful leaf shape and strong fragrance. It is not only a precious aromatic oil tree species but also an important landscape and greening tree species in cities. Cinnamomum camphora'Yongjin' (Cinnamomum camphora'Yongjin', a new species of camphora seed, is a variant species of camphora seed, with bright red branches, golden yellow leaves and flowers, yellow peel, and branches The color of leaves, peels and peels has seasonal changes. As a tall tree with colorful leaves, it has very high potential and value for garden viewing, promotion and application. Ho...

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Problems solved by technology

[0004] In order to solve the problems in the above-mentioned prior art, the present invention mainly fills up the gap in the tissue culture technology of the existing new camphor variety 'Yongjin', aiming at the induction and axillary buds in the regeneration process of the 'Yongjin' plant with stem segments as explants. Difficulty in growth, shedding and black death of new leaves during the subculture and proliferation of axillary buds, and difficulty in rooting clustered buds. Study the culture conditions suitable for each stage of tissue culture and rapid propagation of 'Yongjin', aiming at realizing the rapid growth of 'Yongjin'. Effectively assist in reproduction

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