Chrysanthemum one-step tissue culture quick breeding method
A technology for tissue culture and rapid propagation of chrysanthemums, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as increasing difficulty and complicated operation steps, and achieve the goal of reducing operation difficulty, improving cultivation efficiency, and low production cost Effect
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Embodiment 1
[0017] The chrysanthemum one -step method of cultivation method, it includes the following steps:
[0018] S1. Get the seedlings of chrysanthemum sterile group: collect chrysanthemum outer implants, cut into 2 to 3cm bud stalk sections, sterilizes of alcohol sterilization 30, sterilized sterilization of sterilization of 5 minutes, sterilized water for 5 times, vaccinated in the medium base basisGrowing, such as figure 1 It is shown that the medium is MS+NAA0.2mg / L+IAA0.1mg / L. It is cultivated under the cultivation conditions of 2000lx, 16 h, and temperature 24 ° C; a week later, the new axillary buds germinate to 2 to 2 ~When 3cm, the new medium is used to grow. The medium is still MS+NAA0.2mg / L+IAA0.1mg / L. It is cultivated under the training conditions of 2000LX, 16 h, and temperature 24 ° C.Two weeks after growing, get rooted in a sterile and strong mother, such as figure 2 Show;
[0019] S2, one step fast complication: cut the sterile and strong mother's seedlings obtained in s...
Embodiment 2
[0021] The chrysanthemum one -step method of cultivation method, it includes the following steps:
[0022] S1. Get the seedlings of chrysanthemum sterile group: collect chrysanthemum outer implants, cut into 2 to 3cm bud stalk sections, sterilizes of alcohol sterilization 20s, sterilized sterilization of mercury, washed 4 times with sterile water, vaccinated in the medium base basisGrowing, such as figure 1 It is shown that the medium is MS+NAA0.2mg / L+IAA0.1mg / L. It is cultivated under the cultivation conditions of 2000lx, 16 h, and temperature 24 ° C; a week later, the new axillary buds germinate to 2 to 2 ~When 3cm, the new medium is used to grow. The medium is still MS+NAA0.2mg / L+IAA0.1mg / L. It is cultivated under the training conditions of 2000LX, 16 h, and temperature 24 ° C.Two weeks after growing, get rooted in a sterile and strong mother, such as figure 2 Show;
[0023] S2, one step fast complication: cut the sterile and strong mother's seedlings obtained in step S1 into 2...
Embodiment 3
[0025] The chrysanthemum one -step method of cultivation method, it includes the following steps:
[0026] S1. Get the seedlings of chrysanthemum sterile group: collect chrysanthemum outer implants, cut into 2 to 3cm bud stalk sections, sterilizes 40s of alcohol, sterilizes for mercury, and sterilizes 4 min, so washed 4 times, vaccinated in the medium baseGrowing, such as figure 1 It is shown that the medium is MS+NAA0.2mg / L+IAA0.1mg / L. It is cultivated under the cultivation conditions of 2000lx, 16 h, and temperature 24 ° C; a week later, the new axillary buds germinate to 2 to 2 ~When 3cm, the new medium is used to grow. The medium is still MS+NAA0.2mg / L+IAA0.1mg / L. It is cultivated under the training conditions of 2000LX, 16 h, and temperature 24 ° C.Two weeks after growing, get rooted in a sterile and strong mother, such as figure 2 Show;
[0027] S2, one step fast complication: cut the sterile and strong mother's seedlings obtained in step S1 into 20-30 segments, 2 to 3cm bud...
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