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Method for aseptically seeding ebony and establishing leaf-cutting rapid propagation system

A technology of aseptic sowing and ebony, applied in the field of plant tissue culture, can solve problems such as unsuccessful examples, and achieve the effect of high proliferation coefficient

Inactive Publication Date: 2015-12-16
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But before this, there is no report on the rapid propagation method of ebony tissue culture, and there is no successful example

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The invention provides a method for aseptic seeding and leaf cutting rapid propagation system establishment of ebony, the steps of which are as follows:

[0022] 1), the preparation of culture medium

[0023] (1) Basic medium: MS medium, wherein sucrose 30g / L, agar 7g / L, pH=5.8;

[0024] (2) Proliferation medium: MS+6-BA0.1~0.5mg / L+NAA0.01~0.05mg / L;

[0025] (3) Rooting and strong seedling medium: MS+6-BA0.005~0.01mg / L+NAA0.05~0.2mg / L;

[0026] 2) Disinfection and sowing of seeds

[0027] Take the seeds of ebony as the explant material, put it in a PE centrifuge tube of 1.5ml or 2ml for disinfection treatment, first soak it with detergent solution for 20min, then wash it with tap water, and then wash it with tap water in turn with a volume ratio of 70%. Soak in alcohol solution and sodium hypochlorite solution with an effective chlorine concentration of 1% for 1min and 8min, respectively, and finally rinse with sterile water for 3 to 5 times, each time for 1min (oper...

Embodiment 2

[0033] The invention provides a method for aseptic seeding and leaf cutting rapid propagation system establishment of ebony, the steps of which are as follows:

[0034] 1), the preparation of culture medium

[0035] (1) Basic medium: MS medium, in which sucrose 20-30g / L, agar 5-9g / L, pH=5.8;

[0036] (2) Proliferation medium: MS+6-BA0.3mg / L+NAA0.02mg / L;

[0037] (3) Rooting and strong seedling medium: MS+6-BA0.01mg / L+NAA0.1mg / L;

[0038] 2) Disinfection and sowing of seeds

[0039] Take the seeds of ebony as the explant material, put it in a PE centrifuge tube of 1.5ml or 2ml for disinfection treatment, first soak it with detergent solution for 20min, then wash it with tap water, and then wash it with tap water in turn with a volume ratio of 70%. Soak in alcohol solution and sodium hypochlorite solution with an effective chlorine concentration of 1% for 1min and 8min, respectively, and finally rinse with sterile water for 3 to 5 times, each time for 1min (operated with a pi...

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Abstract

The invention relates to a method for aseptically seeding ebony and establishing a leaf-cutting rapid propagation system. The method comprises the following steps: 1, preparing a culture medium, wherein the culture medium comprises a basic culture medium and components for tissue culturing the culture medium at various stages and specifically includes: (1) a basic culture medium: an MS culture medium; (2) a propagation culture medium; (3) a seedling strengthening and rooting culture medium; 2, sterilizing seeds and culturing the seeds; 3, carrying out the leaf-cutting propagation culture: inoculating a basic part of a mature leaf of a plant in the step 2 onto the propagation culture medium; 4, carrying out the rooting and seedling strengthening culture. The method has the beneficial effects that a plant tissue culture technology is used for seeding and carrying out the leaf-cutting rapid propagation of the ebony, and the propagation coefficient is extremely high; moreover, a greater advantage can be achieved compared with the traditional way of propagating buds by using buds, a great amount of high-quality seedlings consistent in inheritable character can be obtained in a short time, the weakness that the conventional propagation way is slow can be overcome, and positive significance on the mass production and preservation of germplasm resources can be realized.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for aseptic seeding and leaf cutting rapid propagation system establishment of ebony. Background technique [0002] Echeveria agavoides Ebony (Echeveria agavoides Ebony) is a plant of the genus Echeveria in the Sedum family. It is native to Mexico. It has a unique shape, black edges on the jade bottom, and a domineering appearance. It is a rare ornamental succulent. The natural reproduction rate of ebony is very low and the growth rate is extremely slow; at the same time, the International Organization for Succulent Plant Study (The International Organization for Succulent Plant Study) has formulated the "Knowledge of Succulent Plant Cultivation and Collection" in the 1970s in order to protect wild succulent plants from predatory collection or even extinction. "Code of Conduct for Planters", which greatly restricts the introduction of succulents, making the number of ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王燕吕永平牟豪杰陈剑平汪一婷陈志
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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