Tissue culture quick breeding method of lamp stage primula with the red color of morning sunshine

A tissue culture rapid propagation and lampstand technology is applied in the field of plant tissue culture, which can solve the problems of inability to meet production requirements, low reproduction coefficient, and irregular seedling formation, and achieves a shortened seedling formation period, improved reproduction coefficient, and easy seedling formation. Effect

Inactive Publication Date: 2007-08-08
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Primulabeesiana (Primulabeesiana) is a perennial herbaceous flower. Generally, Primulabeesiana is propagated by sowing and branching, but it is mainly propagated by sowing. After sowing in spring, it will bloom

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Using the leaves of Primula beesiana as explants to explore the optimal medium for adventitious bud induction: choose the leaves of well-growing seedlings as explants to explore the optimal medium formula for adventitious bud induction.

[0026] Wash the young leaves of Primula beesiana with tap water for 2-3 hours, drop a drop of detergent in the water and shake for 15 minutes to remove surface impurities, and rinse with tap water repeatedly; surface disinfection: 70% alcohol disinfection for 8-10 seconds, without Rinse with sterile water for 4 to 5 times, disinfect with 0.1% mercury solution for 10 to 15 minutes, and then rinse with sterile water for 4 to 5 times. The sterilized leaves were then cut into 1 cm 2 Small pieces (with part of the veins) were inoculated on the induction medium of clustered buds, 5-6 pieces were inoculated in each bottle, and 10 bottles were connected to each medium, and cultured in the dark. Among them, the concentration gradient of 6-BA: ...

Embodiment 2

[0029] The axillary buds of Primula beesiana (Primula beesiana) were used as explants for primary culture and subculture: select axillary buds from robust plants, rinse them with tap water for 2-3 hours, disinfect and sterilize the leaves, and inoculate them on MS+ 6-BA 2.0mg / l+NAA 0.1mg / l cluster bud induction medium, inoculate 3 shoots per bottle; the light conditions are natural scattered light 3000Lux plus artificial auxiliary light source 1800±200Lux, light time 14h.

[0030] When the axillary buds are inoculated on the differentiation medium, new leaves are continuously pulled out. From 10 days on, the petioles of the new leaves are thick and the base turns red, and light green callus is gradually formed. After 2 weeks, adventitious buds begin to differentiate; 40 days after inoculation , A large number of adventitious buds are differentiated on the newly drawn petioles and leaves of the axillary buds.

[0031] The statistical results show that after 2 months of inoculat...

Embodiment 3

[0033] Rooting culture of tissue-cultured seedlings of Xiahong Dengtai Primula (Primula beesiana): when the adventitious buds grow 2 to 3 leaves on the differentiation medium, they are excised from the callus and transferred to the rooting medium, the formula : In MS, MS+NAA (0.1mg / l, 0.2mg / l, 0.5mg / l), the pH is 5.8; the light conditions are natural scattered light 3000Lux plus artificial auxiliary light source 2500Lux, light time 14h. From the perspective of the time required for rooting, the time required for rooting in medium MS and MS+0.2mg / l NAA is the shortest, 8-10 days, but in MS+0.2mg / l NAA, the rooting amount of tissue culture seedlings And growth is obviously better than MS basic medium, the average height of tissue culture seedlings can reach 4.8cm, and the rooting rate is over 95%.

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Abstract

The invention relates to a method for quickly cultivating the Primula beesiana, which comprises that: selecting the axillary bud of Primula beesiana as explant, to be treated via adventitious induction, generation cultivation, rooting cultivation, and tube seed transfer. With said invention, one axillary bud can be induced into 40-50 buds, while the increment factor is 3-4 via 30-40 days of generation and the rooting rate can reach 95%, and the yield can reach 100%. The invention can improve the seeding ability of orange lamp table (Primula beesiana), shorten the seed time and reduce the cost.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a tissue culture rapid propagation method of Primula beesiana. Background technique [0002] Primula beesiana (Primula beesiana) is a plant of the genus Primula in the Primulaceae family. It is native to the Yunnan region of my country at an altitude of 2700-3000m. Primulabeesiana (Primulabeesiana) is a perennial herbaceous flower. Generally, Primulabeesiana is propagated by sowing and branching, but it is mainly propagated by sowing. After sowing in spring, it will bloom in May of the following year after vegetative growth. , long growth cycle and low reproduction coefficient. Simultaneously, these methods are far from being able to meet the production demand, and the seedlings are not neat. [0003] In order to improve the reproduction coefficient of Primula beesiana and to rapidly propagate fine plant varieties, it is necessary to study the tissue culture of Primula beesiana and form ...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04A01G7/00A01G1/00
Inventor 张启翔李翠娟潘会堂梁树乐程堂仁孙明
Owner BEIJING FORESTRY UNIVERSITY
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