Efficient and rapid tissue culture propagation method of konjac

Abstract

The invention relates to an efficient and rapid tissue culture propagation method of konjac. The efficient and rapid tissue culture propagation method comprises the following steps of: carrying out isolated culture on a konjac corm slicing material and inducing to generate callus, adventitious buds and adventitious roots; and further forming a great mount of konjac tissue culture seedlings within a very short period of time; transplanting the tissue culture seedlings, necessary medicals and fertilizers to a seedbed; and tumbling sprouts normally, and then obtaining a konjac original seed. The efficient and rapid tissue culture propagation method of the konjac, provided by the invention, overcomes the defect that the konjac is incapable of integrating with the market due to low traditional propagation coefficient and high industrial rapid propagation cost, has high propagation coefficient, large propagation amount, a primary pollution rate less than 10% and an average inductivity higher than 90%, shortens a concentrated seedling cycle to three and a half months, can be used for producing seedlings all the year around, producing the original seed from March to July each year under natural conditions, producing the original seed in other time under artificial greenhouse conditions and obtaining the original seeds with an average weight up to 10g or more, greatly lowers the production cost and improves the production benefit.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a high-efficiency and fast konjac seedling and konjac for the large-scale production of the original konjac seed under natural conditions (or artificial greenhouse conditions) by utilizing the in vitro culture technology of plant tissue. Tissue culture propagation method. Background technique [0002] Konjac is a perennial herb of the Araceae family. Konjac has been cultivated in my country for more than 2,000 years. Konjac is a special economic crop that contains a large amount of glucomannan in nature. It has high economic value and great development potential. It is widely used in food, medicine, textile, daily chemicals, printing and dyeing, papermaking, construction, petroleum, environmental protection and other fields. Known as "health food" and "industrial monosodium glutamate". [0003] In recent years, the domestic and foreign konjac market has been further expanded. On t...

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a high-efficiency and rapid tissue culture propagation method for konjac, which provides a high-efficiency and rapid propagation method for the large-scale production of konjac tissue culture seedlings and its original original seeds in each konjac production area, and overcomes the The shortcomings of konjac's traditional low propagation coefficient, mixed species, low traditional konjac propagation coefficient and high cost of full-factory rapid propagation that cannot be connected with the market have been eliminated, and the original species of konjac varieties has been effectively purified and maintained. It is conducive to the production and promotion of improved varieties, with large reproduction coefficient and large reproduction quantity