Method of organic selenium sweet -flavored twisted green

A planting method and technology of Gynostemma pentaphyllum are applied in the planting field of Gynostemma pentaphyllum rich in organic selenium and sweet taste, which can solve the problems of severe coldness, difficulty in entering the mouth, easy injury to the spleen and stomach, etc., and achieve the effects of excellent health care function, improvement of quality and reduction of planting cost.

Active Publication Date: 2018-04-20
湖北省农业科学院农业经济技术研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The original variant of Gynostemma pentaphyllum popularized at home and abroad is extremely bitter in taste, heavy in coldness, difficult to eat, and easily hurts the spleen and stomach, which has become a bottleneck for artificial planting and industrial development of Gynostemma pentaphyllum; Cultivated varieties, research on high-yield and high-efficiency cultivation techniques suitable for their growth and realization of their health care value is blank

Method used

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  • Method of organic selenium sweet -flavored twisted green
  • Method of organic selenium sweet -flavored twisted green
  • Method of organic selenium sweet -flavored twisted green

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] A method for planting Jiaogulan rich in organic selenium and sweet taste, comprising:

[0087] Tissue culture: carried out under the conditions of a temperature of 24°C, a light time of 11h / d, and a light intensity of 1500lx. The explants of Gynostemma pentaphyllum were inoculated into the callus induction medium, and callus tissue was formed on the explants, and then the callus tissue was transferred to the differentiation medium, and clustered buds were differentiated, when the buds of the clustered buds When the seedling grows to about 2 cm, the single sprout is cut off and transferred to the rooting medium, and roots grow out to form tissue cultured seedlings.

[0088] Wherein: the callus induction medium consists of MS medium containing 1mg / L 6-BA, 0.2mg / L NAA, 20g / L sucrose and 6g / L agar in terms of concentration.

[0089] The composition of differentiation medium in terms of concentration was MS medium containing 0.2mg / L NAA, 0.2mg / L 2,4-D, 0.5mg / L 6-BA, 20g / L s...

Embodiment 2

[0096] A method for planting Jiaogulan rich in organic selenium and sweet taste, comprising:

[0097] Explant selection: In this embodiment, before the tissue culture step, explant selection is also included: put the sterilized explants to be selected into the screening medium to cultivate and screen the explants, and the explants to be selected The body includes the shoot tip, stem segments and leaves; the explant is the shoot tip.

[0098] Wherein, the composition of the screening medium in terms of concentration is MS medium containing 1 mg / L 6-BA, 0.1 mg / L NAA, 30 g / L sucrose and 7 g / L agar.

[0099] Disinfection is to rinse the explants with detergent or washing powder with a concentration of 5% for 5 minutes, rinse with running water for 1 hour, then soak in 75% ethanol for 30 seconds, rinse with sterile deionized water for 5 times, and then put the explants in a concentration of 0.1 Shake in % HgCl2 solution for 8 minutes, then rinse with sterile deionized water for 5 ...

Embodiment 3

[0112] A planting method rich in organic selenium sweet Jiaogulan, the steps comprising:

[0113] Explant selection: The explant selection steps provided in this embodiment are basically the same as those provided in Example 2, the difference is that the screening medium for explant selection in this embodiment consists of MS medium containing 1.5mg / L 6-BA, 0.3mg / L NAA, 40g / L sucrose and 8g / L agar.

[0114] Tissue culture: The tissue culture in this example is carried out under the conditions of a temperature of 26° C., an illumination time of 13 h / d, and an illumination intensity of 2000 lx.

[0115] The composition of the callus induction medium in terms of concentration was MS medium containing 2mg / L 6-BA, 0.4mg / L NAA, 40g / L sucrose and 8g / L agar.

[0116] The composition of differentiation medium in terms of concentration was MS medium containing 0.3mg / L NAA, 0.3mg / L 2,4-D, 1.5mg / L 6-BA, 40g / L sucrose and 8g / L agar.

[0117] The rooting medium consists of 2mg / L NAA, 0.4m...

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Abstract

The invention provides a planting method of organic selenium-rich sweet Gynostemma pentaphylla, and belongs to the technical field of cultivation. The planting method of the organic selenium-rich sweet Gynostemma pentaphylla comprises the following steps: selecting varieties of the sweet Gynostemma pentaphylla, carrying out tissue culture on explants of the sweet Gynostemma pentaphylla at first to obtain tissue culture seedlings; then transplanting the tissue culture seedlings in a nursery garden to build a strain garden; planting rhizomes of plants of Gynostemma pentaphylla growing for 1 year in the strain garden to build a propagation expanding garden; planting rhizomes of plants of the Gynostemma pentaphylla growing for 1 year in the propagation expanding garden to build a garden for a land for growing field crops; and planting rhizomes of plants of the Gynostemma pentaphylla growing for 1 year in the garden for the land for growing field crops to the land for growing field crops. The strain garden is built by tissue culture technology, the sweet Gynostemma pentaphylla can be quickly reproduced by tissue culture, the yield of the sweet Gynostemma pentaphylla is increased while viral diseases are reduced, then by building of three-garden reproduction, the tissue culture seedlings subjected to tissue culturing grow well, the quality and yield of the sweet Gynostemma pentaphylla are increased, and high-yield and high-efficiency planting is realized.

Description

technical field [0001] The invention relates to the field of cultivation technology, in particular to a method for planting Gynostemma pentaphylla with rich organic selenium and sweet taste. Background technique [0002] There are 16 species and 3 variants of Gynostemma pentaphyllum in the world, 15 species and 3 variants in my country, and 11 species and 3 variants in Enshi Prefecture, Hubei alone. The original variant of Gynostemma pentaphyllum popularized at home and abroad is extremely bitter in taste, heavy in coldness, difficult to eat, and easily hurts the spleen and stomach, which has become a bottleneck for artificial planting and industrial development of Gynostemma pentaphyllum; Cultivated varieties, high-yield and high-efficiency cultivation technology research suitable for its growth and realizing its health care value are blank. Contents of the invention [0003] The object of the present invention is to provide a planting method of sweet Gynostemma rich in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G22/15C05F11/08
CPCA01G22/00A01H4/001A01H4/008C05F11/08
Inventor 郑威刘金龙
Owner 湖北省农业科学院农业经济技术研究所
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