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Synthetic medium for edible mushrooms and preparation method and application thereof

A technology for synthesizing culture medium and edible fungus, which is applied in botany equipment and methods, applications, fertilizer mixtures, etc. It can solve the problems of limited fruiting ability in the available range, small elasticity of the buffer system, and rapid acidification of the culture medium. Achieve the effect of improving the quality of the strain, stabilizing the quality, and increasing the biomass of mycelium

Active Publication Date: 2016-09-21
辽宁省微生物科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to PDA, Macaya-Lizano synthetic medium is also commonly used to cultivate Pleurotus ostreatus (P.otreatus). The medium has a single carbon and nitrogen source, the buffer system has low elasticity, and the medium acidifies quickly after nitrogen metabolism. has limitations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Use a 10L liquid fermenter to make Pleurotus eryngii (P.eryngii) liquid strains, and prepare 6L of synthetic medium base for edible fungi after emptying according to the operating procedures of the fermenter. The specific dosage is: glucose 60g, maltose 30g, cellobiose 30g, ammonium nitrate 3g, L-aspartic acid 6g, L-serine 1.2g, magnesium sulfate (MgSO4 7H2O) 3g, calcium chloride 1.2g, manganese sulfate (MnSO4 7H2O) 1.2g, zinc sulfate (ZnSO4 . 7H2O) 1.2g, ferrous sulfate (FeSO4 7H2O) 0.6g, copper sulfate (CuSO4 5H2O) 0.6g, potassium dihydrogen phosphate 12g, dipotassium hydrogen phosphate 6g, deionized water 6L, the actual temperature of 121 ℃ for 30min , to obtain solution A. Thiamine hydrochloride (VB1), pyridoxine (VB6), and biotin (VH) were respectively prepared into solutions with a concentration of 10 mg / L, and filtered and sterilized with a sterile filter membrane (≤0.45 μm) to obtain a vitamin stock solution. Cool solution A to 50°C, add vitamin stock solution ...

Embodiment 2

[0029] Prepare medium 1L of the present invention for the screening of Pleurotus ostreatus (P.otreatus) bacterial classification, concrete consumption is: glucose 10g, maltose 5g, cellobiose 5g, ammonium nitrate 0.5g, L-aspartic acid 1g, L-serine 0.2g, magnesium sulfate (MgSO4 7H2O) 0.5g, calcium chloride 0.2g, manganese sulfate (MnSO4 7H2O) 0.2g, zinc sulfate (ZnSO4 7H2O) 0.2g, ferrous sulfate (FeSO4 7H2O) 0.1g, copper sulfate (CuSO4·5H2O) 0.1g, potassium dihydrogen phosphate 2g, dipotassium hydrogen phosphate 1g, agar 16g, pure water 1L, sterilize at 121°C for 15min to obtain solution A. Thiamine hydrochloride (VB1), pyridoxine (VB6), and biotin (VH) were respectively prepared into solutions with a concentration of 10 mg / L, and filtered and sterilized with a sterile filter membrane (≤0.45 μm) to obtain a vitamin stock solution. Cool solution A to 55°C and add vitamin stock solution until the final concentration of thiamine hydrochloride (VB1) is 100 μg / L; the final concentra...

Embodiment 3

[0032] Prepare medium 1L of the present invention for subculture preservation of P. cornucopiae bacterial classification, specific consumption is: glucose 10g, maltose 5g, cellobiose 5g, ammonium nitrate 0.5g, L-aspartic acid 1g , L-serine 0.2g, magnesium sulfate (MgSO4 7H2O) 0.5g, calcium chloride 0.2g, manganese sulfate (MnSO4 7H2O) 0.2g, zinc sulfate (ZnSO4 7H2O) 0.2g, ferrous sulfate (FeSO4 7H2O ) 0.1g, copper sulfate (CuSO4 5H2O) 0.1g, potassium dihydrogen phosphate 2g, dipotassium hydrogen phosphate 1g, agar 16g, pure water 1L, sterilizing at 115°C for 15min to obtain solution A. Thiamine hydrochloride (VB1), pyridoxine (VB6), and biotin (VH) were respectively prepared into solutions with a concentration of 10 mg / L, and filtered and sterilized with a sterile filter membrane (≤0.45 μm) to obtain a vitamin stock solution. Cool solution A to 55°C and add vitamin stock solution until the final concentration of thiamine hydrochloride (VB1) is 100 μg / L; the final concentration...

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Abstract

The invention provides a synthetic medium for edible mushrooms and a preparation method and application thereof. The synthetic medium is prepared from carbon sources including glucose, maltose and cellobiose, nitrogen sources including ammonium nitrate, L-aspartic acid and L-serine, inorganic salts including magnesium sulfate, calcium chloride, manganese sulfate, zinc sulfate, ferric sulfate and copper sulfate, buffer salts including monopotassium phosphate and dipotassium phosphate, and vitamins including thiamine hydrochloride, pyridoxine and biotin. After the carbon sources, the nitrogen sources, the inorganic salts and the buffer salt are dissolved with water, sterilization is performed, a vitamin stock solution is added, and the synthetic medium is obtained through even mixing. The synthetic medium has the advantages of being definite in composition, controllable in quality, ideal in repeatability, especially suitable for Pleurotus sp. edible fungi.

Description

technical field [0001] The invention relates to a synthetic culture medium of edible fungus and its preparation method and application. Background technique [0002] The most commonly used medium in the production of edible fungi is potato dextrose agar (PDA), and the improvement and enrichment on this basis. As a semi-synthetic medium, the source and processing method of raw materials have a relatively large impact on the final product, and it is difficult to control the quality. Due to the limitation of nutrients, the long-term use of PDA to subculture the strains, the phenomenon of species degradation is obvious, which is manifested in the increase of deformed mushrooms and the decrease of transformation rate, etc., and only a few edible fungi can achieve rapid and direct fruiting on PDA. [0003] The composition of the fully synthetic medium is clear and the quality is stable. Although the cost is higher than that of the semi-synthetic medium, it is very necessary to co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G1/00A01G1/04
Inventor 朱巍巍陈飞李莉张疏雨杨云桥邓春海孟庆国陈德伟
Owner 辽宁省微生物科学研究院
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