Tissue culture and rapid propagation inoculation method of anthurium andraeanum

Abstract

The invention designs a tissue culture and rapid propagation inoculation method of anthurium andraeanum. The tissue culture and rapid propagation inoculation method comprises the following steps of (1) stripping adventitious buds obtained through differentiation of callus tissue of the anthurium andraeanum from the callus tissue, and inoculating the stripped adventitious buds to elongation culture mediums or rooting culture mediums or propagation culture mediums for culture, wherein culture conditions include the temperature being 25 DEG C, the illumination intensity being 1500-2500Lux and illumination every day being 15-17h, and performing 40-60 d of culture so as to obtain healthy anthurium andraeanum tissue culture seedlings; and selecting the healthy anthurium andraeanum tissue culture seedlings which are 2cm or above in plant height and have two stalk nodes or above from the obtained healthy anthurium andraeanum tissue culture seedlings, in inoculation disks, performing transection at a position 2mm higher than first nodes of the stalk nodes from bottoms to tops of the seedling stalks to form upper parts and lower parts, enabling the upper parts to be inoculated to the rooting culture mediums, enabling the lower parts to be inoculated to the elongation culture mediums for continuous culture, so as to obtain the anthurium andraeanum tissue culture seedlings which grow orderly, wherein the culture conditions are the same as those in the step 1. According to the tissue culture and rapid propagation inoculation method disclosed by the invention, the problem that plants are uneven to grow in the tissue culture and rapid propagation process of the anthurium andraeanum can be solved.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to an anthurium tissue culture rapid propagation inoculation method. technical background [0002] Anthurium andraeaum, also known as anthurium, anthurium or anthurium, is a perennial evergreen herb of the genus Anthurium in the Araceae family. It is native to the tropical rain forests of South America and is now widely cultivated in Europe, Asia and Africa. Its flowers and leaves are beautiful and elegant, with a unique flower shape, leaf-shaped bracts, and Buddha flame inflorescences. The color is bright and gorgeous, and has great ornamental value. It has a long flowering period and can bloom throughout the year. Happiness, great ambitions, enthusiasm, and blood" have become rare decorative treasures for modern living rooms and various large-scale event venues. [0003] In 2012, 15.22 million pots of anthurium were listed in my country, and in 2013, the market volume was more ...

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Problems solved by technology

[0006] At present, there have been many research reports on the formula of tissue culture rapid propagation medium and cultivation techniques of Anthurium anthurium (Xia Shiyun, et al. Technical research on improving callus induction, plant differentiation and strong seedling rate of anthurium leaves. China Agronomy Bulletin, 2005, 21(2):45-48; Zheng Xueping, et al. Effects of different factors on the proliferation coefficient of Anthurium. Northern Horticulture, 2009, (7):65-68; Guo Yungui, et al. Anthurium callus proliferation Optimization of differentiation conditions. Chinese Horticultural Digest, 2012, 5:10-12; Niu Ruihe, et al. Research on Anthurium callus culture and adventitious bud differentiation influencing factors. Anhui Agricultural Sciences, 2013,41(24):9914-9916 ; Cui Ruifeng, et al. Research on Anthurium leaf callus. Jiangsu Agricultural Sciences, 2013, 41(9):24-26...), but no reports on improving the inoculation method in the rapid propagation process have been retrieved

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