Method for evaluating relieving or repairing effect of cosmetics on skin inflammation

A technology for skin inflammation and cosmetics, applied in the field of cosmetic efficacy evaluation, can solve problems such as the interference of subjective factors in evaluation results, and achieve the effects of convenient evaluation, reproductive cycle period, and large reproductive volume.

Active Publication Date: 2020-12-11
南京新环检测科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Aiming at the problem that the evaluation index selection and processing of the existing evaluation methods are highly subjective, and the subjective factors interfere with the evaluation results, the purpose of the present invention is to provide a method for evaluating the soothing or repairing effect of cosmetics on skin inflammation, so as to realize The evaluation process eliminates subjective interference factors, and the evaluation results are more objective

Method used

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  • Method for evaluating relieving or repairing effect of cosmetics on skin inflammation
  • Method for evaluating relieving or repairing effect of cosmetics on skin inflammation
  • Method for evaluating relieving or repairing effect of cosmetics on skin inflammation

Examples

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Embodiment 1

[0041] A method for evaluating the soothing or repairing effect of cosmetics on skin inflammation. The main component of cosmetics is inositol, comprising the following steps: (1) setting up a normal control group: taking transgenic neutrophil green fluorescent zebrafish as recipients 2 days after fertilization The test object was placed in water for 18 hours;

[0042] (2) Set up the model control group: take the zebrafish with the same specifications as in step (1) as the test object, culture them in water for 18 hours, and add the skin inflammation inducer dodecyl to the water in a water-soluble manner at the beginning of the culture. Sodium sulfonate was added at a concentration of 50 μg / mL; (3) Set up the test group: take the zebrafish with the same specifications as in step (1) as the test object, place them in water for 18 hours, and add them to the water in a water-soluble manner at the beginning of the culture. Add the cosmetic to be tested with the upper limit of the ...

Embodiment 2

[0050] A method for evaluating the soothing or repairing effect of cosmetics on skin inflammation. The main component of cosmetics is squalane, comprising the following steps: (1) setting up a normal control group: taking transgenic neutrophil green fluorescent zebrafish 2 days after fertilization as subjects The test object was placed in water for 18 hours;

[0051] (2) Set up the model control group: take the zebrafish with the same specifications as in step (1) as the test object, culture them in water for 18 hours, and add the skin inflammation inducer dodecyl to the water in a water-soluble manner at the beginning of the culture. Sodium sulfonate, added at a concentration of 50 μg / mL;

[0052] (3) Set up the test group: take the zebrafish with the same specifications as in step (1) as the test object, place them in water for 18 hours, and add the cosmetics to be tested at the upper limit of safe concentration to the water in a water-soluble manner at the beginning of the ...

Embodiment 3

[0060] A method for evaluating the soothing or repairing effect of cosmetics on skin inflammation, the main component of cosmetics is propylene glycol, comprising the following steps: (1) Setting up a normal control group: taking transgenic neutrophil green fluorescent zebrafish 2 days after fertilization as the test object , placed in water for 18 hours;

[0061] (2) Set up the model control group: take the zebrafish with the same specifications as in step (1) as the test object, culture them in water for 18 hours, and add the skin inflammation inducer dodecyl to the water in a water-soluble manner at the beginning of the culture. Sodium sulfonate, added at a concentration of 50 μg / mL;

[0062] (3) Set up the test group: take the zebrafish with the same specifications as in step (1) as the test object, place them in water for 18 hours, and add the cosmetics to be tested at the upper limit of safe concentration to the water in a water-soluble manner at the beginning of the cul...

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Abstract

The invention relates to the technical field of cosmetic efficacy evaluation, and discloses a method for evaluating the efficacy of cosmetics for relieving or repairing skin inflammation in order to solve the problems that in an existing cosmetic skin inflammation relieving evaluation method, the subjectivity of evaluation index selection and treatment is high, and subjective factor interference is brought to an evaluation result. The method comprises the following steps: taking zebra fish as a test object, setting a normal control group, a model control group and a test group, culturing underthe same condition, photographing under a fluorescence microscope, storing a picture, and counting the number of neutrophils in a skin inflammation part of the zebra fish by adopting image processingsoftware based on a statistical method; and then calculating the inflammation relieving or repairing effect (%). Compared with the prior art, the evaluation index data is selected and analyzed by means of instruments and software, and the evaluation result can be quantified; the zebra fish breeding cost is low, the breeding period is short, the breeding amount is large, and the evaluation methodhas the advantages of being efficient, rapid and low in cost.

Description

technical field [0001] The invention relates to the technical field of cosmetic efficacy evaluation, in particular to a method for evaluating the soothing or repairing efficacy of cosmetics on skin inflammation. Background technique [0002] Some cosmetics currently on the market claim to have a soothing or repairing effect on skin inflammation, and the evaluation methods for the soothing effect of cosmetics include: physical and chemical methods, biochemical methods, cell biology methods, three-dimensional reconstruction skin model replacement methods, animal experiments, Subjective evaluation method, semi-subjective evaluation method, objective instrument evaluation method, etc. [0003] Physicochemical method: Physicochemical method is a convenient means to evaluate the effect of cosmetics by testing their physical properties and the content of specific chemical components. At present, the evaluation of soothing efficacy of cosmetics is mostly qualitative and quantitative...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15
CPCG01N33/15Y02A40/81
Inventor 周佳丽郭盟朱晓宇徐懿乔郭胜亚张勇曹兵兵李春启
Owner 南京新环检测科技有限公司
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