A microalgae composition and its application in bullfrog breeding and promoting the growth and metamorphosis of bullfrog tadpoles

Abstract

The present invention discloses a microalgae composition and its application in bullfrog breeding and promoting the growth and metamorphosis of bullfrog tadpoles. The present invention optimizes the nutritional structure of bullfrog feed by adding a specific microalgae composition, promotes the weight growth and metamorphosis of bullfrogs in the tadpole stage, shortens the time of adult frogs, improves the efficiency and success rate of metamorphosis, reduces reproductive losses, saves feed, reduces feeding costs, and also promotes the development of the digestive system and immune system of bullfrogs, maintains the health of the digestive tract, improves the digestion capacity of feed, and enhances immunity. The present invention not only improves the breeding efficiency of bullfrogs, but also reduces the dependence on chemical additives, is conducive to environmental protection and green sustainable development, and can also promote the wide application of microalgae in aquaculture, bringing new development opportunities to related industries.

Description

Technical Field

[0001] The invention relates to the technical field of animal feed, in particular to a microalgae composition and application thereof in bullfrog breeding and promoting the growth and metamorphosis of bullfrog tadpoles. Background Art

[0002] Bullfrogs are widely farmed as economic frogs, and are favored by the market for their delicious meat and rich nutrition. Bullfrogs need to go through two stages from fertilized eggs to adult frogs: tadpole stage and metamorphosis stage. In the tadpole stage, bullfrogs mainly feed on plankton and plant bait, and gradually change to preying on small aquatic animals as their bodies grow. The metamorphosis stage is a critical period for bullfrogs to transform from tadpole form to adult frog form. During this stage, the demand for nutrition is extremely high, and diseases and death are prone to occur.

[0003] Promoting the rapid and healthy growth of larvae into adults is the key to improving bullfrog farming efficiency. However, the traditional feed used in bullfrog farming has insufficient or unbalanced nutrients, low digestion and absorption efficiency, and the addition of hormones that are easy to remain. It cannot meet the needs of bullfrog tadpoles for various nutrients during metamorphosis and development, and it is difficult to achieve the purpose of accurately controlling the feed nutrient supply of bullfrogs at different developmental stages, resulting in slow growth of bullfrog tadpoles, low metamorphosis rate, decreased immunity, and low survival rate of young frogs. Therefore, how to improve the feed formula to improve the efficiency of bullfrog farming is a hot topic in this field. Summary of the invention

[0004] In order to solve the problems that the bullfrog feed in the prior art cannot accurately meet the nutritional needs of bullfrogs at different growth stages, especially the metamorphosis period, resulting in slow growth of bullfrog tadpoles, low metamorphosis rate, decreased immunity, low survival rate of young frogs, etc., the present invention provides a microalgae composition and its application in bullfrog breeding and promoting the growth and metamorphosis of bullfrog tadpoles.

[0005] The first object of the present invention is to provide a microalgae composition.

[0006] The second object of the present invention is to provide the use of the microalgae composition in accelerating the metamorphosis of bullfrog tadpoles.

[0007] The third object of the present invention is to provide the use of the microalgae composition in improving the success rate of metamorphosis of bullfrog tadpoles.

[0008] The fourth object of the present invention is to provide use of the microalgae composition in promoting the development of the digestive system of bullfrogs.

[0009] The fifth object of the present invention is to provide the use of the microalgae composition in improving the immunity of bullfrogs.

[0010] The sixth object of the present invention is to provide the use of the microalgae composition in any two or three of accelerating the metamorphosis of bullfrog tadpoles, improving the success rate of metamorphosis of bullfrog tadpoles, promoting the development of the bullfrog digestive system or improving the immunity of bullfrogs.

[0011] The seventh object of the present invention is to provide the use of the microalgae composition in accelerating the metamorphosis of bullfrog tadpoles, improving the success rate of metamorphosis of bullfrog tadpoles, promoting the development of the bullfrog digestive system and improving the immunity of bullfrogs.

[0012] The eighth object of the present invention is to provide the use of the microalgae composition in the preparation of a bullfrog feed additive and / or a bullfrog feed having any one, two, three or four functions of accelerating the metamorphosis of bullfrog tadpoles, improving the success rate of metamorphosis of bullfrog tadpoles, promoting the development of the bullfrog digestive system or improving the immunity of bullfrogs.

[0013] The ninth object of the present invention is to provide a bullfrog feed additive or bullfrog feed.

[0014] In order to achieve the above object, the present invention is implemented by the following scheme:

[0015] Microalgae such as Chlorella, Spirulina and Schizochytrium contain rich nutrients and have a positive impact on the growth and health of aquatic animals. The present invention enriches the nutrients in traditional bullfrog feed by combining a specific ratio of Chlorella, Spirulina and Schizochytrium, providing necessary and accurate nutritional support for the growth and development of bullfrogs in the tadpole stage and metamorphosis stage.

[0016] A microalgae composition is composed of Chlorella, Spirulina and Schizochytrium in a mass ratio of (2-4):(4-12):(2-4).

[0017] Preferably, the mass ratio of the Chlorella vulgaris to the Schizochytrium sp. is 1:1.

[0018] More preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 1:(2-3):1.

[0019] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 3:8:3.

[0020] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 2:4:2.

[0021] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 4:12:4.

[0022] The use of any of the microalgae compositions in accelerating the metamorphosis of bullfrog tadpoles should also be within the protection scope of the present invention. Accelerating the metamorphosis of bullfrog tadpoles is to shorten the metamorphosis period of bullfrog tadpoles and improve their metamorphosis efficiency.

[0023] Preferably, the microalgae composition is used for feeding bullfrog tadpoles and / or bullfrog larvae.

[0024] Preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3%wt to 10%wt.

[0025] More preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9%wt to 9.3%wt.

[0026] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 3:8:3, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 6.6 wt %.

[0027] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 2:4:2, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9wt%.

[0028] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 4:12:4, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 9.3wt%.

[0029] The use of any of the microalgae compositions in improving the success rate of metamorphosis of bullfrog tadpoles should also be within the scope of protection of the present invention. The success rate of metamorphosis of bullfrog tadpoles is the ratio of bullfrog tadpoles that successfully develop into young frogs to all bullfrog tadpoles cultured, and the bullfrog tadpoles that successfully develop into young frogs do not include bullfrog tadpoles that die during the metamorphosis process and bullfrog tadpoles with deformed development.

[0030] Preferably, the microalgae composition is used for feeding bullfrog tadpoles and / or bullfrog larvae.

[0031] Preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3%wt to 10%wt.

[0032] More preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9%wt to 9.3%wt.

[0033] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 3:8:3, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 6.6 wt %.

[0034] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 2:4:2, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9wt%.

[0035] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 4:12:4, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 9.3wt%.

[0036] The use of any of the microalgae compositions in promoting the development of the bullfrog digestive system should also be within the protection scope of the present invention.

[0037] Preferably, the microalgae composition is used to increase the content of digestive enzymes in the intestine of bullfrogs.

[0038] More preferably, the digestive enzymes include amylase, lipase and protease.

[0039] Preferably, the microalgae composition is used for feeding bullfrog tadpoles and / or bullfrog larvae.

[0040] Preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3%wt-10%wt.

[0041] More preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9%wt to 9.3%wt.

[0042] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 3:8:3, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 6.6 wt %.

[0043] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 2:4:2, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9wt%.

[0044] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 4:12:4, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 9.3wt%.

[0045] The use of any of the microalgae compositions in improving the immunity of bullfrogs should also be within the protection scope of the present invention.

[0046] Preferably, the microalgae composition is used to increase the content of antioxidant enzymes in bullfrogs.

[0047] More preferably, the antioxidant enzyme comprises superoxide dismutase.

[0048] Preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3%wt to 10%wt.

[0049] More preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9%wt to 9.3%wt.

[0050] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 3:8:3, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 6.6 wt %.

[0051] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 2:4:2, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9wt%.

[0052] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 4:12:4, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 9.3wt%.

[0053] The use of any of the microalgae compositions in any two of accelerating the metamorphosis of bullfrog tadpoles, improving the success rate of metamorphosis of bullfrog tadpoles, promoting the development of the bullfrog digestive system or improving the immunity of bullfrogs should also be within the protection scope of the present invention.

[0054] Preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3%wt to 10%wt.

[0055] More preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9%wt to 9.3%wt.

[0056] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 3:8:3, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 6.6 wt %.

[0057] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 2:4:2, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9wt%.

[0058] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 4:12:4, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 9.3wt%.

[0059] The use of any of the microalgae compositions in any three of accelerating the metamorphosis of bullfrog tadpoles, improving the success rate of metamorphosis of bullfrog tadpoles, promoting the development of the bullfrog digestive system, or improving the immunity of bullfrogs should also be within the protection scope of the present invention.

[0060] Preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3%wt-10%wt.

[0061] More preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9%wt to 9.3%wt.

[0062] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 3:8:3, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 6.6 wt %.

[0063] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 2:4:2, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9wt%.

[0064] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 4:12:4, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 9.3wt%.

[0065] The use of any of the microalgae compositions in accelerating the metamorphosis of bullfrog tadpoles, improving the success rate of metamorphosis of bullfrog tadpoles, promoting the development of the bullfrog digestive system and improving the immunity of bullfrogs should also be within the protection scope of the present invention.

[0066] Preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3%wt-10%wt.

[0067] More preferably, the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9%wt to 9.3%wt.

[0068] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 3:8:3, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 6.6 wt %.

[0069] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 2:4:2, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 3.9wt%.

[0070] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 4:12:4, and the content of the microalgae composition in the bullfrog feed additive or bullfrog feed is 9.3wt%.

[0071] A bullfrog feed additive comprises 3%wt-10%wt of the microalgae composition.

[0072] Preferably, the microalgae composition comprises 3.9%wt to 9.3%wt.

[0073] More preferably, the microalgae composition comprises 3.9%wt.

[0074] More preferably, the microalgae composition comprises 6.6 wt %.

[0075] More preferably, the microalgae composition comprises 9.3%wt.

[0076] Preferably, the mass ratio of the Chlorella vulgaris to the Schizochytrium sp. is 1:1.

[0077] More preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 1:(2-3):1.

[0078] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 3:8:3, and the content of the microalgae composition in the bullfrog feed additive is 6.6 wt %.

[0079] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 2:4:2, and the content of the microalgae composition in the bullfrog feed additive is 3.9wt%.

[0080] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 4:12:4, and the content of the microalgae composition in the bullfrog feed additive is 9.3wt%.

[0081] The use of any one of the bullfrog feed additives in accelerating the metamorphosis of bullfrog tadpoles, improving the success rate of metamorphosis of bullfrog tadpoles, promoting the development of the bullfrog digestive system or improving the immunity of bullfrogs should also be within the protection scope of the present invention.

[0082] A bullfrog feed comprises 3%wt-10%wt of the microalgae composition.

[0083] Preferably, the microalgae composition comprises 3.9%wt to 9.3%wt.

[0084] More preferably, the microalgae composition comprises 3.9%wt.

[0085] More preferably, the microalgae composition comprises 6.6 wt %.

[0086] More preferably, the microalgae composition comprises 9.3%wt.

[0087] Preferably, the mass ratio of the Chlorella vulgaris to the Schizochytrium sp. is 1:1.

[0088] More preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 1:(2-3):1.

[0089] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 3:8:3, and the content of the microalgae composition in the bullfrog feed is 6.6 wt %.

[0090] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 2:4:2, and the content of the microalgae composition in the bullfrog feed is 3.9wt%.

[0091] Further preferably, the microalgae composition consists of Chlorella, Spirulina and Schizochytrium in a mass ratio of 4:12:4, and the content of the microalgae composition in the bullfrog feed is 9.3wt%.

[0092] Preferably, the bullfrog feed also contains protein feed, sugars and oils.

[0093] More preferably, the protein feed comprises animal protein feed and plant protein feed.

[0094] Further preferably, the animal protein feed comprises fish meal and chicken meal.

[0095] Further preferably, the plant protein feed includes soybean meal, rapeseed meal and cottonseed meal.

[0096] More preferably, the sugar comprises corn starch.

[0097] More preferably, the oils and fats include animal oils and fats and vegetable oils and fats.

[0098] More preferably, the animal fat comprises fish oil.

[0099] More preferably, the vegetable oil includes soybean oil.

[0100] More preferably, the feed further comprises vitamins and minerals.

[0101] The vitamins include, but are not limited to, vitamin A, vitamin B, vitamin C, vitamin D, vitamin E and vitamin K.

[0102] Further preferably, the vitamins are complex vitamins for frogs, including vitamin A, vitamin D3, vitamin E2, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, inositol, pantothenic acid, choline, folic acid, biotin and vitamin C1.

[0103] The minerals include, but are not limited to, calcium salts, phosphorus salts, sodium salts, potassium salts, iodine salts, magnesium salts, iron salts and zinc salts.

[0104] Further preferably, the minerals are composite minerals for frogs, including CaCO3, KH2PO4, MgSO4·7H2O, NaCl, Fe(II) gluconate, MnSO4·7H2O, ZnSO4·7H2O, CuSO4·5H2O, KI, CoCl2·6H2O, NH4 molybdate and NaSeO3.

[0105] More preferably, the feed comprises the fish meal, the chicken meal, the soybean meal, the rapeseed meal, the cottonseed meal, the corn starch, the fish oil, the soybean oil, the complex vitamins and the complex minerals, and the mass ratios thereof are (18-22): (14-18): (55-65): (30-34): (28-32): (27-30): (2-4): (2-4): (1-3): (1-3).

[0106] Still further preferably, the bullfrog feed comprises the fish meal, the chicken meal, the soybean meal, the rapeseed meal, the cottonseed meal, the corn starch, the fish oil, the soybean oil, the complex vitamins and the complex minerals, and the mass ratios thereof are 20:16:60:32:30:29:3:3:2:2 respectively.

[0107] In some specific embodiments, the bullfrog feed comprises the fish meal, the chicken meal, the soybean meal, the rapeseed meal, the cottonseed meal, the corn starch, the fish oil, the soybean oil, the complex vitamins, the complex minerals and the microalgae composition, and the mass ratios thereof are 20:16:60:32:30:29:3:3:2:2:14, respectively.

[0108] In some specific embodiments, the bullfrog feed comprises the fish meal, the chicken meal, the soybean meal, the rapeseed meal, the cottonseed meal, the corn starch, the fish oil, the soybean oil, the complex vitamins, the complex minerals and the microalgae composition, and the mass ratios thereof are 20:16:60:32:30:29:3:3:2:2:8, respectively.

[0109] In some specific embodiments, the bullfrog feed comprises the fish meal, the chicken meal, the soybean meal, the rapeseed meal, the cottonseed meal, the corn starch, the fish oil, the soybean oil, the complex vitamins, the complex minerals and the microalgae composition, and the mass ratios thereof are 20:16:60:32:30:29:3:3:2:2:20, respectively.

[0110] The use of any one of the bullfrog feeds in any one, two, three or four of accelerating the metamorphosis of bullfrog tadpoles, improving the success rate of metamorphosis of bullfrog tadpoles, promoting the development of the bullfrog digestive system or improving the immunity of bullfrogs should also be within the protection scope of the present invention.

[0111] Compared with the prior art, the present invention has the following beneficial effects:

[0112] The present invention optimizes the nutritional structure of bullfrog feed by adding a specific microalgae composition, promotes the weight growth and metamorphosis of bullfrogs in the tadpole stage, shortens the time for adult frogs, improves the efficiency and success rate of metamorphosis, reduces reproductive losses, saves feed, reduces feeding costs, and also promotes the development of the digestive system and immune system of bullfrogs, maintains the health of the digestive tract, improves feed digestion ability, and enhances immunity. The present invention not only improves the breeding efficiency of bullfrogs, but also reduces the dependence on chemical additives, is beneficial to environmental protection and green sustainable development, and can also promote the wide application of microalgae in aquaculture, bringing new development opportunities to related industries. DETAILED DESCRIPTION

[0113] The present invention is further described in detail below in conjunction with specific examples, which are only used to explain the present invention and are not used to limit the scope of the present invention. The test methods used in the following examples are conventional methods unless otherwise specified; the materials, reagents, etc. used are reagents and materials that can be obtained from commercial channels unless otherwise specified. The composite vitamins used in the feeds of the following examples and comparative examples are commercially available vitamin premixes (mg / kg), containing 500,000 IU of vitamin A, 500,000 IU of vitamin D3, 500 mg of vitamin E2, 1000 mg of vitamin K3, 5000 mg of vitamin B1, 5000 mg of vitamin B2, 5000 mg of vitamin B6, 5000 μg of vitamin B12, 25000 mg of inositol, 10000 mg of pantothenic acid, 10000 mg of choline, 10000 mg of folic acid, 250 mg of biotin, and 10000 mg of vitamin C); the composite minerals used are commercially available mixed minerals (g / kg), containing 314.0 g of CaCO3, 469.3 g of KH2PO4, 147.4 g of MgSO4·7H2O, 49.8 g of NaCl, 10.9 g of Fe(II) gluconate, and 3.12 g of MnSO4·7H2O. g, ZnSO4·7H2O4.67 g, CuSO4·5H2O0.62 g, KI0.16 g, CoCl2·6H2O0.08 g, NH4 molybdate0.06 g and NaSeO30.02 g.

[0114] Example 1 A bullfrog feed with added microalgae

[0115] The formula of the bullfrog feed is: 20g of fish meal, 16g of chicken meal, 60g of soybean meal, 32g of rapeseed meal, 30g of cottonseed meal, 29g of corn starch, 3g of fish oil, 3g of soybean oil, 2g of complex vitamins, 2g of complex minerals, and 14g of a microalgae composition; wherein the microalgae composition consists of 3g of Chlorella, 8g of Spirulina, and 3g of Schizochytrium.

[0116] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0117] Example 2 A bullfrog feed with added microalgae

[0118] The formula of the bullfrog feed is: 20g of fish meal, 16g of chicken meal, 60g of soybean meal, 32g of rapeseed meal, 30g of cottonseed meal, 29g of corn starch, 3g of fish oil, 3g of soybean oil, 2g of complex vitamins, 2g of complex minerals, and 8g of microalgae composition; wherein the microalgae composition consists of 2g of Chlorella, 4g of Spirulina, and 2g of Schizochytrium.

[0119] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0120] Example 3 A bullfrog feed with added microalgae

[0121] The formula of the bullfrog feed is: 20g of fish meal, 16g of chicken meal, 60g of soybean meal, 32g of rapeseed meal, 30g of cottonseed meal, 29g of corn starch, 3g of fish oil, 3g of soybean oil, 2g of complex vitamins, 2g of complex minerals, and 20g of a microalgae composition; wherein the microalgae composition consists of 4g of Chlorella, 12g of Spirulina, and 4g of Schizochytrium.

[0122] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0123] Comparative Example 1 A conventional bullfrog feed

[0124] The formula of the bullfrog feed is: 20g fish meal, 16g chicken meal, 60g soybean meal, 32g rapeseed meal, 30g cottonseed meal, 29g corn starch, 3g fish oil, 3g soybean oil, 2g complex vitamins, and 2g complex minerals.

[0125] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0126] Comparative Example 2: A bullfrog feed with added chlorella

[0127] The formula of the bullfrog feed is: 20g fish meal, 16g chicken meal, 60g soybean meal, 32g rapeseed meal, 30g cottonseed meal, 29g corn starch, 3g fish oil, 3g soybean oil, 2g complex vitamins, 2g complex minerals, and 14g chlorella.

[0128] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0129] Comparative Example 3: A bullfrog feed with spirulina added

[0130] The formula of the bullfrog feed is: 20g fish meal, 16g chicken meal, 60g soybean meal, 32g rapeseed meal, 30g cottonseed meal, 29g corn starch, 3g fish oil, 3g soybean oil, 2g complex vitamins, 2g complex minerals, and 14g spirulina.

[0131] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0132] Comparative Example 4: A bullfrog feed with added Schizochytrium

[0133] The formula of the bullfrog feed is: 20g fish meal, 16g chicken meal, 60g soybean meal, 32g rapeseed meal, 30g cottonseed meal, 29g corn starch, 3g fish oil, 3g soybean oil, 2g complex vitamins, 2g complex minerals, and 14g Schizochytrium.

[0134] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0135] Comparative Example 5: A bullfrog feed with added microalgae

[0136] The formula of the bullfrog feed is: 20g of fish meal, 16g of chicken meal, 60g of soybean meal, 32g of rapeseed meal, 30g of cottonseed meal, 29g of corn starch, 3g of fish oil, 3g of soybean oil, 2g of complex vitamins, 2g of complex minerals, and 14g of a microalgae composition; wherein the microalgae composition consists of 3.8g of chlorella and 10.2g of spirulina.

[0137] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0138] Comparative Example 6: A bullfrog feed with added microalgae

[0139] The formula of the bullfrog feed is: 20g of fish meal, 16g of chicken meal, 60g of soybean meal, 32g of rapeseed meal, 30g of cottonseed meal, 29g of corn starch, 3g of fish oil, 3g of soybean oil, 2g of complex vitamins, 2g of complex minerals, and 14g of microalgae composition; wherein the microalgae composition consists of 7g of Chlorella vulgaris and 7g of Schizochytrium schizochytrium.

[0140] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0141] Comparative Example 7 A bullfrog feed with added microalgae

[0142] The formula of the bullfrog feed is: 20g of fish meal, 16g of chicken meal, 60g of soybean meal, 32g of rapeseed meal, 30g of cottonseed meal, 29g of corn starch, 3g of fish oil, 3g of soybean oil, 2g of complex vitamins, 2g of complex minerals, and 14g of a microalgae composition; wherein the microalgae composition consists of 10.2g of spirulina and 3.8g of Schizochytrium.

[0143] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0144] Comparative Example 8: A bullfrog feed with added microalgae

[0145] The formula of the bullfrog feed is: 20g of fish meal, 16g of chicken meal, 60g of soybean meal, 32g of rapeseed meal, 30g of cottonseed meal, 29g of corn starch, 3g of fish oil, 3g of soybean oil, 2g of complex vitamins, 2g of complex minerals, and 14g of a microalgae composition; wherein the microalgae composition consists of 3g of Chlorella, 3g of Spirulina, and 8g of Schizochytrium.

[0146] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0147] Comparative Example 9: A bullfrog feed with added microalgae

[0148] The formula of the bullfrog feed is: 20g of fish meal, 16g of chicken meal, 60g of soybean meal, 32g of rapeseed meal, 30g of cottonseed meal, 29g of corn starch, 3g of fish oil, 3g of soybean oil, 2g of complex vitamins, 2g of complex minerals, and 14g of a microalgae composition; wherein the microalgae composition consists of 3g of Haematococcus pluvialis, 8g of Spirulina, and 3g of Schizochytrium.

[0149] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0150] Comparative Example 10: A bullfrog feed with added hormones

[0151] The formula of the bullfrog feed is: 20g fish meal, 16g chicken meal, 60g soybean meal, 32g rapeseed meal, 30g cottonseed meal, 29g corn starch, 3g fish oil, 3g soybean oil, 2g complex vitamins, 2g complex minerals, and 0.03mg / g testosterone.

[0152] After weighing each component according to the above formula, mixing and preparing by conventional method is obtained.

[0153] Application Example 1 Evaluation of Bullfrog Breeding Effect

[0154] The bullfrog tadpoles used in this experiment were all from Guangzhou Guanxing Agricultural Technology Co., Ltd., and the experiment was carried out at the Zhongshan Innovation Base of South China Agricultural University.

[0155] Bullfrog tadpoles (weighing about 0.02 g) with similar development, good health and no obvious deformities were randomly divided into 12 groups, each with 120 tadpoles. Each group was fed with the feeds of Examples 1 to 3 and Comparative Examples 1 to 10 in turn, and conventional feeding management was carried out under the same environmental conditions, and feeding was carried out according to conventional methods, and continued to be raised until complete metamorphosis.

[0156] During the rearing period, the growth, metamorphosis process and health status of bullfrog tadpoles in each group were regularly observed, and the Gosner staging method was used to determine the growth stage of the tadpoles. When the tail of the bullfrog tadpole completely disappeared and turned into a young frog, the metamorphosis was completed.

[0157] The body length, weight, date of becoming a young frog, death and deformity of the bullfrog tadpoles were recorded in time. When the bullfrogs were reared for 80 days (the metamorphosis rate of half of the groups exceeded 50%), the final weight gain, weight gain rate, specific growth rate, fatness, feed coefficient and frog rate of each group of bullfrogs were counted. When the end of the rearing period was reached (all groups of tadpoles completed metamorphosis), the final weight gain, weight gain rate, specific growth rate, fatness, feed coefficient, frog rate, immunity physiological and biochemical indicators (superoxide dismutase) and intestinal health-related physiological and biochemical indicators (amylase, lipase and protease) of each group of bullfrogs were counted.

[0158] 2. Test results

[0159] Table 1 Effects of different feeds on the growth of bullfrog tadpoles for 80 days

[0160]

[0161] Note: Final weight gain (g) = final weight (g) - initial weight (g); Weight gain rate (%) = final weight gain (g) ÷ initial weight (g) × 100%; Specific growth rate (% / day) = {ln (final weight (g)) - ln (initial weight (g))} ÷ number of days of feeding × 100%; Fatness (g / cm 3 )=weight(g)÷body length(cm) 3 ; Feed coefficient (%) = weight of feed consumed during feeding (g) ÷ final weight gain (g) × 100%.

[0162] As shown in Table 1, after 80 days of feeding, compared with the bullfrog feeds of Comparative Examples 1 to 10, the final weight gain, weight gain rate and specific growth rate of the bullfrog tadpoles fed with the bullfrog feeds of Examples 1 to 3 were significantly higher, and the feed coefficients were significantly lower; except for the bullfrog feed of Comparative Example 3, the fatness of the bullfrog tadpoles fed with the other feeds was higher than 0.9, and the differences between the groups were small.

[0163] The above results show that adding a microalgae composition consisting of a specific proportion of Chlorella, Spirulina and Schizochytrium to the feed can significantly promote the weight gain of bullfrog tadpoles, promote growth and development, save feed and reduce feeding costs.

[0164] Table 2 Metamorphosis of bullfrog tadpoles after 80 days of feeding with different feeds

[0165]

[0166] Note: Frog maturity rate = number of young frogs (individuals) ÷ 120 (tails) × 100%.

[0167] As shown in Table 2, after 80 days of feeding, compared with the bullfrog feeds of Comparative Examples 1 to 10, the bullfrog tadpoles fed with the bullfrog feeds of Examples 1 to 3 had a significantly higher rate of becoming frogs, and the earliest days to becoming frogs were significantly shorter.

[0168] The above results show that adding a microalgae composition consisting of a specific proportion of Chlorella, Spirulina and Schizochytrium to the feed can promote more bullfrog tadpoles to complete metamorphosis and become young frogs earlier, shorten the time for frogs to become adults, and improve the efficiency of metamorphosis.

[0169] Table 3 Metamorphosis of bullfrog tadpoles after feeding with different feeds to the end of the feeding period

[0170]

[0171] Note: Metamorphosis rate (%) = number of adult frogs ÷ 120 (tails) × 100%; Mortality rate (%) = number of dead tadpoles ÷ 120 (tails) × 100%; Deformity rate (%) = number of deformed tadpoles ÷ 120 (tails) × 100%.

[0172] As shown in Table 3, when the feeding end point is reached, compared with the bullfrog feeds of Comparative Examples 1 to 10, the metamorphosis rate of bullfrog tadpoles fed with the bullfrog feeds of Examples 1 to 3 is significantly higher, all exceeding 94%, and the mortality rate and deformity rate are significantly lower, both lower than 4%, and both lower than 3%; although the metamorphosis rate of bullfrog tadpoles fed with the frog feed of Comparative Example 8 is as high as 91.7%, the mortality rate and deformity rate are both higher than 4%, which shows that when the proportion of Schizochytrium in the microalgae composition is too large, adverse side effects are likely to occur.

[0173] The above results show that adding a microalgae composition consisting of a specific proportion of Chlorella, Spirulina and Schizochytrium to the feed can promote the metamorphosis process of bullfrog tadpoles, improve the success rate of metamorphosis and reduce reproductive losses.

[0174] Table 4 Effects of different feeds on bullfrog immunity and intestinal health-related physiological indicators until the end of feeding

[0175]

[0176] As shown in Table 4, when the feeding end point was reached, the bullfrogs fed with the bullfrog feeds of Examples 1 to 3 contained higher levels of amylase, lipase, protease and superoxide dismutase in their bodies compared with the bullfrog feeds of Comparative Examples 1 to 10.

[0177] The above results show that adding a microalgae composition consisting of a specific proportion of Chlorella, Spirulina and Schizochytrium to the feed can promote the development of the digestive and immune systems of bullfrog tadpoles during their development, maintain the health of the digestive system, improve feed digestion ability, and enhance immunity.

[0178] In summary, the present invention achieves the promotion of the growth and development of bullfrog tadpoles, the acceleration of the metamorphosis process and the improvement of the success rate of metamorphosis by adding a specific microalgae composition to the feed, and also promotes the development and health of the digestive system of bullfrogs and enhances immunity. Compared with adding a single type of microalgae, two types of microalgae, other combinations of microalgae (such as replacing Chlorella with Haematococcus pluvialis in Comparative Example 9) or commonly used hormones (such as testosterone in Comparative Example 10), the present invention has more significant multiple functions and is more green and safe.

[0179] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. For ordinary technicians in this field, other different forms of changes or modifications can be made based on the above descriptions and ideas. It is not necessary and impossible to list all the implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. An application of a microalgae composition in accelerating the metamorphosis of bullfrog tadpoles, characterized in that: The microalgae composition is composed of Chlorella, Spirulina and Schizochytrium in a mass ratio of (2-4): (4-12): (2-4), and bullfrog tadpoles are fed with feed added with the microalgae composition; the bullfrog feed contains fish meal, chicken meal, soybean meal, rapeseed meal, cottonseed meal, corn starch, fish oil and soybean oil; the content of the microalgae composition in the bullfrog feed is 3%wt-10%wt; the application is for the purpose of non-disease treatment.

2. An application of a microalgae composition in improving the success rate of metamorphosis of bullfrog tadpoles, characterized in that: The microalgae composition is composed of Chlorella, Spirulina and Schizochytrium in a mass ratio of (2-4): (4-12): (2-4), and bullfrog tadpoles are fed with feed added with the microalgae composition; the bullfrog feed contains fish meal, chicken meal, soybean meal, rapeseed meal, cottonseed meal, corn starch, fish oil and soybean oil; the content of the microalgae composition in the bullfrog feed is 3%wt-10%wt; the application is for the purpose of non-disease treatment.

3. Use of a microalgae composition in promoting the development of the digestive system of bullfrogs, characterized in that: The microalgae composition is composed of Chlorella, Spirulina and Schizochytrium in a mass ratio of (2-4): (4-12): (2-4), and bullfrog tadpoles are fed with feed added with the microalgae composition; the bullfrog feed contains fish meal, chicken meal, soybean meal, rapeseed meal, cottonseed meal, corn starch, fish oil and soybean oil; the content of the microalgae composition in the bullfrog feed is 3%wt-10%wt; the application is for the purpose of non-disease treatment.

4. A use of a microalgae composition for accelerating the metamorphosis of bullfrog tadpoles, improving the success rate of metamorphosis of bullfrog tadpoles, or promoting the development of the digestive system of bullfrogs, characterized in that: The microalgae composition is composed of Chlorella, Spirulina and Schizochytrium in a mass ratio of (2-4): (4-12): (2-4), and bullfrog tadpoles are fed with feed added with the microalgae composition; the bullfrog feed contains fish meal, chicken meal, soybean meal, rapeseed meal, cottonseed meal, corn starch, fish oil and soybean oil; the content of the microalgae composition in the bullfrog feed is 3%wt-10%wt; the application is for the purpose of non-disease treatment.

5. The use according to any one of claims 1 to 4, characterized in that: The content of the microalgae composition in bullfrog feed is 3.9%wt to 9.3%wt.

6. Use of a microalgae composition in preparing a bullfrog feed having any one, two or three functions of accelerating the metamorphosis of bullfrog tadpoles, improving the success rate of metamorphosis of bullfrog tadpoles or promoting the development of the digestive system of bullfrogs, characterized in that: The microalgae composition is composed of Chlorella, Spirulina and Schizochytrium in a mass ratio of (2-4): (4-12): (2-4); the bullfrog feed contains fish meal, chicken meal, soybean meal, rapeseed meal, cottonseed meal, corn starch, fish oil and soybean oil; the content of the microalgae composition in the bullfrog feed is 3%wt-10%wt.

7. A bullfrog feed, characterized in that: The bullfrog feed comprises 3%wt to 10%wt of a microalgae composition, wherein the microalgae composition is composed of Chlorella, Spirulina and Schizochytrium in a mass ratio of (2-4): (4-12): (2-4); Also includes fish meal, chicken meal, soybean meal, canola meal, cottonseed meal, corn starch, fish oil and soybean oil.

8. The bullfrog feed according to claim 7, characterized in that: Also contains vitamins and minerals.

9. The bullfrog feed according to claim 8, characterized in that: The vitamins are complex vitamins for frogs, including vitamin A, vitamin D3, vitamin E2, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, inositol, pantothenic acid, choline, folic acid, biotin and vitamin C1.

10. The bullfrog feed according to claim 8, characterized in that: The minerals are composite minerals for frogs, including CaCO3, KH2PO4, MgSO4·7H2O, NaCl, Fe(II) gluconate, MnSO4·7H2O, ZnSO4·7H2O, CuSO4·5H2O, KI, CoCl2·6H2O, NH4 molybdate and NaSeO3.

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