Cryptomeria fortunei tissue culture rapid-propagation method

A technology of tissue culture and cedar, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of basic medium and combined hormone selective culture experiments, unsuitable for individual reproduction and cultivation of cedar with excellent genotypes, etc. , to achieve the effects of large-scale production, short cultivation period and large reproduction

Inactive Publication Date: 2013-01-09
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] Zhang Cuiping (2008) preliminarily researched its tissue culture rapid propagation technology, but this study used Cedar seed embryos as explants, which was not suitable for the reproduction and culture of Cedar individuals with excellent genotypes, and only MS was used in the experiment. Medium, the addition of exogenous hormones only adopted a single factor design, and no selective culture experiments were carried out on other basic medium and combined hormones

Method used

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  • Cryptomeria fortunei tissue culture rapid-propagation method
  • Cryptomeria fortunei tissue culture rapid-propagation method
  • Cryptomeria fortunei tissue culture rapid-propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select a robust cedar stem section, cut off the needles on the stem section, cut the stem section into about 4cm, wash it with running water for 1~2h, sterilize it in 75% ethanol for 30s, and then use 50% 84 disinfectant (Contains a small amount of Tween-20, add one drop per 50mL) Disinfect for 10 minutes, rinse with sterile water 8 times, then cut the stems into about 2cm, inoculate in medium for inducing adventitious buds, and start to produce adventitious buds after 8 days of light-induced culture ; After the adventitious buds are produced, select the young shoots that are growing and strong and transfer them to the rooting medium 1, and then cultivate them in the dark for 2-15 days, then put them under the light and cultivate them for 2-30 days, and then transfer them to the rooting medium 2. 10d Started rooting. Among them, the medium for inducing adventitious buds is: DCR+0.1~2.0mg / L 6-BA+0.1~2.0mg / L IBA+0.001~0.01mg / L TDZ+0.5~5.0g / L AC; rooting medium 1 is : 1 / 2...

Embodiment 2

[0024] Repeat the experiment according to the same steps as described in Example 1. At each induction culture stage, different doses of activated carbon (0.5-10.0 g / L) were added respectively, wherein 0.5-5.0 g / L of activated carbon was added to the medium for inducing adventitious buds, Adding 1.0-10.0 g / L of activated carbon to rooting medium 1 and adding 0.5-5.0 g / L to rooting medium 2 can obviously promote the growth and robustness of Cedar cedar, with green leaves, and effectively prevent the occurrence of yellowing and browning of seedlings. The induction rooting phase promotes rooting.

Embodiment 3

[0026] Repeat the experiment according to the same steps described in Example 1. In the rooting induction stage, the macroelements in the 1 / 2DCR medium used are reduced by 1 / 2 compared with conventional DCR. The results are shown in Table 1, which can significantly promote the induction of rooting and increase the rooting rate to 85%.

[0027] Table 1 Effects of macroelements in medium on rooting of Chinese cedar

[0028]

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Abstract

The invention discloses a cryptomeria fortunei tissue culture rapid-propagation method. The method comprises taking a cryptomeria fortunei explant stem segment, inoculating the cryptomeria fortunei explant stem segment on an inducing adventitious bud culture medium which comprises DCR+ 0.1-2.0mg/L of 6-BA+ 0.1-2.0mg/L of IBA+ 0.5-5.0g/L of AC+ 0.001-0.01mg/L of TDZ+ 30g/L of cane sugar, and performing inducing culture to generate adventitious buds; adopting two stages to take root for rooting culture, namely first placing a rooting culture medium 1 which comprises 1/2 of DCR+ 0.1-2.0mg/L of NAA+ 0.1-2.0mg/L of IBA+0.1-1.0mg/L of 6-BA+ 1.0-10.0g/L of AC+ 10.0-30.0g/L of cane sugar, first performing dark culture for 2-15d, performing culture for 5-30d under illumination, and transferring to a rooting culture medium 2 which comprises DCR+ 0.1-1.0mg/L of NAA+ 0.1-1.0mg/L of 6-BA+ 0.5-5.0g/L of AC+ 30.0g/L of cane sugar. By the method, the tender bud propagation coefficient is over 5.7, and the rooting rate is over 80%. The culture cycle is short, the propagation quantity is large, and the cryptomeria fortunei tissue culture rapid-propagation method is favorable for large-scale production.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture propagation, in particular to a rapid propagation method of cedar tissue culture, in particular to a method of using cedar stems as explant materials, using a self-made medium to first induce cedar to produce adventitious buds, and then Rooting was induced to obtain regenerated plants. Background technique [0002] Cedar ( Cryptomeria fortunei ) for the genus Cryptomeriaceae ( Cryptomeria D. Don ), alias peacock fir, tree, up to 4m, diameter at breast height up to more than 2m; bark reddish brown, fibrous, split into long strips and fall off; large branches are nearly whorled, flat or oblique; branchlets are slender, often drooping, green, The leaves in the middle of the branches are longer, and often gradually become shorter towards the ends. The leaves are subulate and slightly curved inward, with stomatal lines on the four sides, 1-1.5 cm long. The leaves of fruiting branches a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 诸葛强沙玉清徐立安王立科王章荣王福生
Owner NANJING FORESTRY UNIV
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