Tissue culture quick breeding method of orange red lamp stage primula

Abstract

The invention relates to a method for quickly cultivating the orange lamp table (Primula bulleyana), comprising explant selection, surface disinfection, adventitious induction, generation cultivation, rooting cultivation, and tube seed transfer. With said invention, one axillary bud can be induced into 40-50 buds, while the increment factor is 3-4 via 30-40 days of generation and the rooting rate can reach 95%, and the yield can reach 100%. The invention can improve the seeding ability of orange lamp table (Primula bulleyana), shorten the seed time and reduce the cost.

Description

technical field

[0001] The invention relates to plant tissue culture, in particular to a tissue culture rapid propagation method of Primula bulleyana. Background technique

[0002] Primula bulleyana (Primula bulleyana) is a plant of the genus Primula in the Primulaceae family. It is a very beautiful garden ornamental flower. Primula bulleyana is a perennial herbaceous flower. The whole plant is in the shape of a rosette, with well-developed rhizomes and 5 or more leaf clusters growing upwards. It is mainly propagated by seeding. Generally, after sowing in spring, it grows vegetatively It begins to flower in May of the following year, grows slowly, and has a low reproduction coefficient. In addition to seed propagation, the available methods for asexual propagation of Primula are: branch (leaf cluster) propagation, tissue culture propagation, and a small number of perennial species can be propagated by cuttings, but the method of branch propagation is far from meeting produc...