Methods of screening agents for activity using teleosts

a screening agent and teleost technology, applied in the field of screening agents for activity using teleosts, can solve the problem of increasing cell death activity and the response is not good enough

Inactive Publication Date: 2006-06-08
PHYLONIX PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In some such methods, the response is an increase in cell death activity.

Method used

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  • Methods of screening agents for activity using teleosts
  • Methods of screening agents for activity using teleosts
  • Methods of screening agents for activity using teleosts

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[0393] A. Procedure for High Throughput Screening

[0394] 1. Synchronized embryos are distributed into 96-well assay plate. Embryos developmental stage (age) can be synchronized by treating the adult fish with propidium iodide, and stimulating them to lay egg in a predicted time frame. Embryos can be distributed using an automated particle handling devices equipped with a light scattering detection system (BD Biosciences, Bedford, Mass.). The particle handling device should preferably be capable of dispensing up to 600 μm particles. Alternatively a liquid dispenser fitted with large bore pipette tip can be used to dispense embryos into microtiter wells automatically.

[0395] 2. 100 μl of diluted compounds are added to each well (in fish water). An automatic microplate pippetting and washing workstation (Zymark, Hopkinton, Mass.; Packard Bioscience, Groningen, The Netherlands) can be used. A simple bar code system to identify each plate (Decitek, Westborough, Mass.).

[0396] 3. Plates a...

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Abstract

The present invention provides methods of screening an agent for activity using teleosts. Methods of screening an agent for angiogenesis activity, toxic activity and an effect cell death activity in teleosts are provided. The invention further provides high throughput methods of screening agents in multi-well plates.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] The present application is a continuation-in-part of U.S. Ser. No. 09 / 255,397 filed Feb. 22, 1999, which derives priority from U.S. Provisional Patent Application Ser. No. 60 / 075,783, filed on Feb. 23, 1998, and U.S. Provisional Patent Application Ser. 60 / 100,950, filed on Sep. 18, 1998, each of which is incorporated herein by reference in its entirety for all purposes. Commonly owned copending U.S. Patent Application Ser. No. 60 / 110,464, filed Dec. 1, 1998 is directed to related subject matter and is incorporated herein by reference in its entirety for all purposes.STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT [0002] This invention was supported by a grant from the National Institutes of Health (Grant No. 1R43CA7938-01). The Government may have certain rights in this invention.BACKGROUND OF THE INVENTION [0003] Currently, searches for target-specific therapeutic and prophylactic compounds ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01K67/027A61K49/00C12N15/85G01N33/50
CPCA01K2217/05A01K2227/40A01K2267/035A01K2267/0393A61K49/0008A61K49/006C12N15/8509G01N33/5014G01N33/5088G01N2333/4603G01N2510/00A61K49/0021
Inventor SERBEDZIJA, GEORGE N.SEMINO, CARLOSFROST, DEANNA M.
Owner PHYLONIX PHARMA
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