The invention provides a synthesis method of desmopressin. The method comprises the following steps: coupling amino acid monomers and Mpa onto a solid carrier, from which the protective groups have been moved, in a water solution according to a sequence of PG-Gly-OH, PG-D-Arg-OH, PG-Pro-OH, PG-Cys(Trt)-OH, PG-Asn-OH, PG-Gln-OH, PG-Phe-OH, PG-Tyr-OH, and Mpa, wherein the PG represents a water-soluble amino protective group, oxidizing, cracking, and purifying. The provided synthesis method does not use any organic solvent, the cost is saved, the environment is protected, moreover, part of the side-chain functional groups in the peptide sequence does not need to be protected, the operation is simple, the cost is low, the yield is high, and thus the provided synthesis method can generate considerable economic benefits, is very practical, and has a wide application prospect.