Rapid analysis method of biogenic amine in fish

An analytical method and rapid analysis technology, applied in the field of analytical chemistry and food safety, to achieve the effects of stable derivatives, wide linear range and high sensitivity

Active Publication Date: 2018-03-09
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far there is no report of applying the method of the present invention to the analysis of biogenic amines in fish tissue

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  • Rapid analysis method of biogenic amine in fish
  • Rapid analysis method of biogenic amine in fish
  • Rapid analysis method of biogenic amine in fish

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Embodiment 1

[0020] Evaluation of analysis methods for biogenic amines in fish tissues based on 5-sulfosalicylic acid-based bead grinding extraction, benzoyl chloride derivatization, and liquid chromatography-triple quadrupole mass spectrometry detection

[0021] Investigation of linearity, detection limit and quantification limit. Concentrations of single-labeled mother liquors of agmatine, histamine, tyramine, spermine, spermidine, 2-phenethylamine, tryptamine, cadaverine, putrescine, 1-heptylamine and 1,7-heptanediamine Both are 10mg / mL, 1-heptylamine and 1,7-heptanediamine are internal standards, and the remaining 9 amines are biogenic amines to be analyzed. Except tryptamine, tyramine and 2-phenylethylamine, ethanol is used as the solvent, and acetonitrile solution with a volume concentration of 20% is selected as the solvent for other substances. The concentration of the mixed-standard mother liquor of 9 kinds of biogenic amines is 1 mg / mL (the quality of each bio-amine is the same): 1...

Embodiment 2

[0031] Analysis of biogenic amines in four types of fish from different sources

[0032] (1) Sample pretreatment: The fish tissues of the mackerel, yellow croaker, hairtail, and pomfret purchased from the two retail markets are respectively skinned, deboned, and visceral. The fish tissues are coarsely homogenized with a cooking machine (rotating speed is 12000 rpm / The homogenization time is 1-5min). After coarse homogenization, weigh 20mg±0.5mg of fish tissues and place them in a 2mL ep tube, add zirconia beads in sequence, 300μL of 5-sulfosalicylic acid solution with a concentration of 5mg / mL, and 100μL of each with a concentration of 4.5μg / mL of heptamine and 1,7-heptanediamine internal standard solution, 100μL of acetonitrile solution with a volume concentration of 20%; use a hybrid grinder to grind and extract for 1 min under 20Hz conditions, repeat twice; grind and extract the sample immediately Put it in a centrifuge and centrifuge for 10 min at 4°C and 14000 rpm; take 1...

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Abstract

The invention discloses a rapid analysis method of biogenic amine in fish. The common biogenic amine (nine types of biogenic amine comprising agmatine, histamine, tyramine, spermine, spermidine, 2-phenylethylamine, tryptamine, cadaverine and putrescine) is analyzed in a targeted manner by taking 5-sulfosalicylic acid as an extraction solvent, adopting bead agitating and grinding as an extraction method, taking benzoyl chloride as a derivatization reagent and adopting an LC (liquid chromatogram)-triple tandem quadrupole mass spectrometry. The biogenic amine is detected in a multi-reactions monitoring manner by taking ion pairs with optimum response, which are obtained by optimization, as quantitative ion pairs and taking ion pairs with following response as auxiliary qualitative ion pairs.According to the method, the sample extraction time and the biogenic amine derivation time can be greatly shortened, so that the analysis time of the whole analysis process is greatly shortened. The analysis method has the advantages of being simple in sample pretreatment, rapid in derivation, high in detection sensitivity, high in repeatability and the like.

Description

Technical field [0001] The invention relates to the field of analytical chemistry and food safety, and is a rapid analysis method for biogenic amines in fish. Background technique [0002] Biogenic amines are a type of nitrogen-containing low molecular weight organic compounds with biological activity. Common biological amines mainly include agmatine, histamine, tyramine, spermine, spermidine, 2-phenylethylamine, tryptamine, cadaverine, and putrescine. In organisms, decarboxylase produced by microorganisms acts on free amino acids to cause their decarboxylation reaction to produce corresponding biogenic amines. They are mostly found in animals and plants, fermented foods and meat foods. Biogenic amines are an essential part of biologically active cells and have important biological functions. However, excessive intake can cause headaches, nausea, allergies and other adverse reactions, and even life-threatening in severe cases. Therefore, the content of biogenic amine in food c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 许国旺傅燕青赵春霞路鑫周智慧
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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