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Silk fibroin-gelatin double crosslinked hydrogel and preparation method thereof

A technology of silk fibroin and hydrogel, which is applied in the field of biomedical materials, can solve problems such as complex preparation process and complex mold, and achieve the effect of simple and easy to operate preparation process, strong mechanical properties, and favorable large-scale industrial production

Inactive Publication Date: 2019-01-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current preparation process of hollow hydrogels is relatively complicated, which may involve the preparation of complex molds, which limit its application.

Method used

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  • Silk fibroin-gelatin double crosslinked hydrogel and preparation method thereof
  • Silk fibroin-gelatin double crosslinked hydrogel and preparation method thereof
  • Silk fibroin-gelatin double crosslinked hydrogel and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Preparation of silk fibroin-gelatin double cross-linked hydrogel

[0051] (1) Dissolve 10g of gelatin completely in 5mM 2-morpholineethanesulfonic acid biological buffer at 50°C, add 5g of tyramine hydrochloride after the solution is cooled to room temperature, and then add 1-(3- Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 0.3675g and N-hydroxysuccinimide 0.1103g, stirred and reacted at room temperature for 12h, after the reaction was completed, the dialysis bag with a molecular weight cut-off of 10000 was dialyzed for 4 days, Freeze-drying at -50°C to obtain tyramide-modified gelatin;

[0052] The H NMR spectra of unmodified gelatin and tyramide-modified gelatin are as follows: figure 1 As shown, the absorption peak of the modified gelatin in the chemical shift range of 6.6~7.1 indicates the hydrogen atom on the phenol group on the tyramide radical, which shows that the modified gelatin has successfully grafted tyramide for enzymatic cross-linking reaction....

Embodiment 2

[0058] Preparation of silk fibroin-gelatin double cross-linked hydrogel

[0059] (1) Dissolve 10g of gelatin completely in 5mM 2-morpholineethanesulfonic acid biological buffer at 50°C, add 5g of tyramine hydrochloride after the solution is cooled to room temperature, and then add 1-(3- Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 0.3675g and N-hydroxysuccinimide 0.1103g, stirred and reacted at room temperature for 12h, after the reaction was completed, the dialysis bag with a molecular weight cut-off of 10000 was dialyzed for 4 days, Freeze-drying at -50°C to obtain tyramide-modified gelatin;

[0060] See figure 1 As shown, the absorption peak of the modified gelatin in the chemical shift range of 6.6~7.1 indicates the hydrogen atom on the phenol group on the tyramide radical, which shows that the modified gelatin has successfully grafted tyramide for enzymatic cross-linking reaction. root group.

[0061] (2) Soak 4g of degummed silk in 20ml of bromination soluti...

Embodiment 3

[0066] Preparation of silk fibroin-gelatin double cross-linked hydrogel

[0067] (1) Dissolve 10g of gelatin completely in 5mM 2-morpholineethanesulfonic acid biological buffer at 50°C, add 5g of tyramine hydrochloride after the solution is cooled to room temperature, and then add 1-(3- Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 0.3675g and N-hydroxysuccinimide 0.1103g, stirred and reacted at room temperature for 12h, after the reaction was completed, the dialysis bag with a molecular weight cut-off of 10000 was dialyzed for 4 days, Freeze-drying at -50°C to obtain tyramide-modified gelatin;

[0068] See figure 1 As shown, the absorption peak of the modified gelatin in the chemical shift range of 6.6~7.1 indicates the hydrogen atom on the phenol group on the tyramide radical, which shows that the modified gelatin has successfully grafted tyramide for enzymatic cross-linking reaction. root group.

[0069] (2) Soak 4g of degummed silk in 20ml of bromination soluti...

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Abstract

The invention discloses a silk fibroin-gelatin double crosslinked hydrogel and a preparation method thereof. The method comprises the following steps: (1) preparing tyramine grafting modified gelatin;(2) preparing a silk fibroin solution; (3) preparing thermoreversible silk fibroin / tyramine modified gelatin hydrogel; and (4) preparing silk fibroin-gelatin double crosslinked hydrogel. The method of the invention has simple process, and the prepared double crosslinked silk fibroin-gelatin composite hydrogel has stronger mechanical properties than the gelatin or silk fibroin hydrogel alone, hasthe advantages of gelatin or silk fibroin hydrogel, and makes up for the defects of gelatin or silk fibroin hydrogel. The hydrogel with excellent biocompatibility and mechanical properties has a broadapplication prospect in the field of tissue engineering vascularization.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a silk fibroin-gelatin double crosslinked hydrogel and a preparation method thereof. Background technique [0002] Hydrogel is a kind of soft material with high water content. It is composed of a three-dimensional network formed by the combination of hydrophilic polymer and water. It is very similar to the composition and structure of human soft tissue. It has been widely studied as a tissue engineering repair material. Its application fields include drug sustained release, repair of soft tissues such as cartilage, blood vessels, muscles and nerves. However, the gel products currently used for tissue repair still face many problems, including poor mechanical strength, biocompatibility, and mismatch between the degradation rate in vivo and the regeneration rate of new tissue. [0003] Gelatin is a hydrophilic natural polymer material obtained from the degradation of ...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08J3/24C08L89/00
CPCC08J3/075C08J3/243C08J2389/00C08J2489/00
Inventor 曹晓东徐晟
Owner SOUTH CHINA UNIV OF TECH
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