Adenosine detecting method based on micro-fluidic chip and nucleic acid adapter technology

Abstract

The invention discloses an adenosine detecting method based on a micro-fluidic chip and a nucleic adapter technology. Biotin-modified adenosine nucleotide adapter and biotin-modified casein are fixed on the surface of an avidin-modified microsphere to form a functional microsphere through a biotin-avidin combining method; capture probes modified with horseradish peroxidase and sulfydryl are modified on nanometer particles as signal marks; functional nanometer particles flow to a micro-fluidic microsphere array to detect a detection area of a chip and hybrid with a microsphere array; after elution, an adenosine solution flows to be incubated with hybridized microspheres; a peroxidase substrate solution flows in; biotinylated tyramine is combined with the casein on the surface of the microsphere; the avidin-marked quantum dot flows in after elution; qualification and quantification are carried out on the adenosine by calculating a ratio of a fluorescence amount of the microsphere surface after adenosine inflows and a fluorescence amount of the microsphere surface before the adenosine inflows. The adenosine detecting method provided by the invention can be used for detecting and analyzing a target at high sensitivity, so that the specific and accurate quantitative analysis of the adenosine in a serum sample is realized.

Description

technical field [0001] The invention belongs to the technical field of biological detection of a micro-total analysis system, and relates to an adenosine detection method based on a microfluidic chip and nucleic acid aptamer recognition technology. Background technique [0002] Adenosine is an endogenous nucleoside, which has received extensive attention and research because of its important role in the expression of signals in the central nervous system and peripheral nervous system. At the same time, adenosine is the substrate of adenosine deaminase, and its content change will also indirectly reflect the metabolic level of adenosine deaminase in vivo, and the detection of adenosine deaminase activity is an important reference for the diagnosis of many clinical diseases Therefore, the development of new detection methods for adenosine is of great significance for medical clinical diagnosis and body metabolism. Traditional adenosine detection methods mainly include HPLC, u...

AI Technical Summary

Problems solved by technology

Traditional adenosine detection methods mainly include HPLC, ultraviolet detection, flow cytometry and fluorescence analysis. These technologies have limited detection sensitivity and require a large volume of detection solution, which limits their application in the field of high-sensitivity microanalysis.

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