Method for extracting high-quality collagen at low temperature

A collagen, high-quality technology, applied in the direction of fermentation, etc., can solve the problems of large energy, destruction of collagen's natural biological activity, pollution and consumption of the environment, and achieve the effect of saving equipment

Inactive Publication Date: 2010-12-22
JIANGSU ANHUI BIO TECH
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  • Abstract
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Problems solved by technology

[0003] Because acid-base treatment pollutes the environment, enzymatic hydrolysis is usually carried out at a high temperature of about 60 degrees. The current extraction method not only seriously pollutes the environment and consumes a lot of energy, but also destroys the natural biological properties of collagen. active

Method used

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Examples

Experimental program
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Embodiment Construction

[0019] A method for low-temperature extraction of high-quality collagen, comprising the following steps:

[0020] (1) Choose fresh or dry fish scales, carry out enzymolysis treatment with enzymolysis solution, the temperature of enzymolysis reaction is 4~49 ℃ (example 4 ℃, 20 ℃, 40 ℃, 49 ℃); the time of enzymolysis reaction is 1-19 hours (eg 1 hour, 9 hours, 19 hours), the pH of the enzymolysis solution is 3-9 (eg 3, 6, 9), if not in this range, HCl or NaOH can be used to adjust. The amount of enzymolysis liquid is 1~19 times of fish scale weight (for example 1 time, 5 times, 9 times); 10:100); the enzyme is one or a mixture of metalloprotease, sulfhydryl protease, zinc metalloprotease, serine carboxypeptidase, cathepsin, serine protease, collagenase, endopeptidase, ficin, acid protease, etc. .

[0021] (2) After the enzymolysis treatment, the enzymolysis solution is filtered to obtain collagen liquid, the filtration accuracy range is 0.15 μm-150 μm, and the filtrate is drie...

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Abstract

The invention discloses a method for extracting high-quality collagen at the low temperature, comprising the following steps of: taking fresh or dry fish scales and the like, and carrying out enzymolysis treatment with an enzymolysis liquid, wherein the temperature of the enzymolysis reaction in between 4 DEG C and 49 DEG C; and filtering the enzymolysis liquid after the enzymolysis treatment to obtain a collagen liquid, and drying to obtain collagen powder. The invention has the advantages of security, convenience and environment protection, and enables the activity of the collagen to be reserved to the utmost extent.

Description

Technical field: [0001] The invention relates to a method for extracting high-quality collagen. Background technique: [0002] Collagen is one of the most important proteins in the human body, and it has been widely used in medicine, biomaterials, food, daily chemical industry and other fields. At present, the main means of extracting collagen in various countries in the world is to adopt strong acid and strong alkali, high temperature treatment and enzymatic hydrolysis, etc., and a large number of hidden raw materials of mad cow disease and foot-and-mouth disease pathogens such as pig, beef bone and tendon are used. [0003] Because acid-base treatment pollutes the environment, enzymatic hydrolysis is usually carried out at a high temperature of about 60 degrees. The current extraction method not only seriously pollutes the environment and consumes a lot of energy, but also destroys the natural biological properties of collagen. active. Invention content: [0004] The p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 郁小兵
Owner JIANGSU ANHUI BIO TECH
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