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Functional near-infrared fluorescence nanoparticles and preparation and application thereof

A fluorescent nanometer and infrared fluorescence technology, applied in the field of nanometer near-infrared fluorescent materials, can solve the problems of inactive groups that are not easily labeled with molecules, low fluorescence intensity, weak photostability, etc. Easy to disintegrate effect

Active Publication Date: 2015-06-17
ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the indocyanine green molecule itself has low fluorescence intensity, weak photostability, inactive groups and difficult molecular labeling, and poor targeting for in vivo imaging.

Method used

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  • Functional near-infrared fluorescence nanoparticles and preparation and application thereof
  • Functional near-infrared fluorescence nanoparticles and preparation and application thereof
  • Functional near-infrared fluorescence nanoparticles and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation method of near-infrared fluorescent nanoparticles:

[0034] (1) Indocyanine Green (ICG) - chitosan (CA) electrostatic binding

[0035] Add ICG to 0.1M HAc / NaAc buffer solution (pH4.2), prepare 1mg / mL ICG solution, and remove a small amount of insoluble matter through 0.22μm filter membrane to obtain a solution. Use HAc / NaAc buffer solution (0.1M, pH 4.2) to prepare 5mg / mL chitosan solution, figure 1 It is the gel exclusion chromatography result of CA, and its molecular weight is calculated as 25.5kDa based on the standard curve obtained from the standard dextran, and it is treated with a 0.22μm filter membrane to obtain a solution. In the state of magnetic stirring, 50 μL of ICG solution was dropped into 2 mL of chitosan solution through a liquid inlet pump at a constant speed to prepare an ICG-CA electrostatic binding solution.

[0036](2) Preparation of indocyanine green (ICG)-chitosan (CA)-sodium polyphosphate (STPP) nanoparticles

[0037] Add sodium p...

Embodiment 2

[0041] Characterization of ICG-CA-STPP-ALG near-infrared fluorescent nanoparticles:

[0042] (1) Shape and size of ICG-CA-STPP-ALG near-infrared fluorescent nanoparticles

[0043] figure 2 It is a transmission electron microscope photo of ICG-CA-STPP-ALG near-infrared fluorescent nanoparticles. The results show that indocyanine green can form nanoparticles after being electrostatically wrapped by nanocarriers such as chitosan and sodium alginate, and the dispersion between nanoparticles Good performance, little condensation occurs. image 3 It is the particle size distribution of ICG-CA-STPP-ALG near-infrared fluorescent nanoparticles observed by electron microscope, and the statistical analysis results show that the particle size distribution of the nanoparticles is ~9nm. The particle size can be controlled by adjusting the ratio of CA to STPP, CA concentration, stirring speed, pump speed, etc.

[0044] (2) Effect of different concentrations of NaCl on the particle size o...

Embodiment 3

[0051] Imaging of mice using ICG-CA-STPP-ALG near-infrared fluorescent nanoparticles: After the mice were anesthetized with anesthesia, 7.5 μl / g of body weight of ICG was injected into the tail vein of the mice at a concentration of (83 μg / mL) ICG-CA -STPP-ALG near-infrared fluorescent nanoparticles, using a small animal in vivo imaging system to perform fluorescence imaging under the excitation of 770nm excitation light, the fluorescence imaging conditions are 780nm-950nm, the step size is 10nm, and the exposure time is 400ms. Figure 7 The left column is the fluorescence imaging images of nude mice at different time points after injection of free ICG, and the right column is the fluorescence imaging images of nude mice at different time points after injection of ICG-CA-STPP-ALG nanoparticles. It can be seen from the results that after 1 minute of injection of ICG-CA-STPP-ALG near-infrared fluorescent nanoparticles, the surface skin of the mouse emitted a stronger fluorescent ...

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Abstract

The invention discloses preparation of near-infrared fluorescence nanoparticles and an application method of the near-infrared fluorescence nanoparticles in an imaging technology. A loaded near-infrared fluorescent dye is taken as a luminescence center, and chitosan and polylysine are taken as basic skeletons, so as to prepare the near-infrared fluorescence nanoparticles by self-assembling package of sodium alginate into a shell. A transmission electron microscope displays the mean grain sizes of the near-infrared fluorescence nanoparticles are about 15nm; a dynamic scattering test indicates that the mean dynamic hydration radius is less than 160nm; the photostability of the near-infrared fluorescence nanoparticles is significantly enhanced in comparison with fluorescent dye molecules; the near-infrared fluorescence nanoparticles can stably exist in 0-1.5% NaCl aqueous solution.

Description

technical field [0001] The invention relates to a nanometer near-infrared fluorescent material, in particular to a functional nanometer near-infrared fluorescent particle and its preparation and application. Background technique [0002] Bioanalytical chemistry at the nanoscale is the most important development direction of nanobiotechnology. In nanobiotechnology, in vivo fluorescence imaging technology based on near-infrared fluorescent nanoprobes is one of the research frontiers and important development directions in the field of international bioanalysis science, and it is also a research hotspot in various countries. Functional nano-fluorescent materials are a new growth point in the research of nano-materials in recent years. At present, their application focus is mainly in the field of biotechnology, such as biological imaging detection, in-situ tracer of biological macromolecules, fluorescence microscopy detection, immunohistochemistry, Cytochemistry, etc. The repo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C09K11/02C12Q1/68G01N21/64A61K49/00
Inventor 马小军谭明乾吴昊
Owner ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
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