Dual-emission-ratio fluorescent carbon dots as well as preparation method and application thereof

A ratiometric fluorescence and dual-emission technology, applied in luminescent materials, fluorescence/phosphorescence, chemical instruments and methods, etc., can solve the problems of high cost, complicated operation and processing, and time-consuming

Active Publication Date: 2021-05-28
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing ratiometric fluorescent probes are mainly constructed by combining two nanomaterials with different fluorescence emission wavel

Method used

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  • Dual-emission-ratio fluorescent carbon dots as well as preparation method and application thereof
  • Dual-emission-ratio fluorescent carbon dots as well as preparation method and application thereof
  • Dual-emission-ratio fluorescent carbon dots as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] One for the detection of Fe3+ The preparation method of the dual-emission ratio fluorescent carbon dot comprises the steps:

[0038] Weigh 30 g of sodium alginate and 1 g of glutathione and dissolve them in 150 mL of deionized water, stir magnetically at room temperature for 30 min to completely dissolve the solids, and transfer the resulting mixed system to a microwave synthesizer with a polytetrafluoroethylene liner , heated to 100°C at a heating rate of 4°C / min, and reacted for 24 hours. After the reaction stopped, the program dropped to room temperature, and then the product obtained was vacuum filtered through a 0.22 μm filter membrane, and the obtained filtrate was dialyzed with a 3500Da dialysis bag for 120 hours, and collected The dialysate is finally freeze-dried to obtain dual-emission ratio fluorescent carbon dots. The absolute fluorescence quantum yield of the prepared dual-emission ratio fluorescent carbon dots was measured by a fluorescence spectrometer, a...

Embodiment 2

[0040] One for the detection of Fe 3+ The preparation method of the dual-emission ratio fluorescent carbon dot comprises the steps:

[0041] Weigh 1g of sodium alginate and 30g of glutathione and dissolve them in 200mL of DMF, stir magnetically at room temperature for 5min to completely dissolve the solids, transfer the resulting mixed system to a microwave synthesizer with a polytetrafluoroethylene liner, Heat to 220°C at a heating rate of 20°C / min, and react for 0.5h. After the reaction stops, the program lowers to room temperature, and then the product obtained is vacuum filtered through a 0.22μm filter membrane. The dialysate is finally freeze-dried to obtain dual-emission ratio fluorescent carbon dots. The absolute fluorescence quantum yield of the prepared dual-emission ratio fluorescent carbon dots was measured by a fluorescence spectrometer, and the fluorescence quantum yield was 6.8%.

Embodiment 3

[0043] One for the detection of Fe 3+ The preparation method of the dual-emission ratio fluorescent carbon dot comprises the steps:

[0044] Dissolve 10 g of sodium alginate and 10 g of glutathione in a mixed solution of 200 mL of deionized water / DMF (volume ratio 3 / 1), stir magnetically at room temperature for 10 min to completely dissolve the solid, and transfer the resulting mixed system to Put it into a microwave synthesizer with a polytetrafluoroethylene liner, heat it up to 190°C at a heating rate of 10°C / min, and react for 8 hours. After the reaction stops, the program will drop to room temperature, and then vacuum filter the obtained product through a 0.22 μm filter. Dialyze the resulting filtrate with a 3500Da dialysis bag for 90 hours, collect the dialysate, and finally freeze-dry to obtain the dual-emission ratio fluorescent carbon dots. The absolute fluorescence quantum yield of the prepared dual-emission ratio fluorescent carbon dots was measured by a fluorescenc...

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Abstract

The invention discloses a dual-emission-ratio fluorescent carbon dot as well as a preparation method and application thereof. According to the preparation method of the dual-emission-ratio fluorescent carbon dots, sodium alginate and glutathione are subjected to hydrothermal carbonization or solvothermal carbonization to prepare the dual-emission-ratio fluorescent carbon dots. The method is simple and easy to operate, low in cost and good in repeatability. The excitation wavelength of the prepared carbon dots is 420 nm, and the dual emission wavelengths are 480 nm and 650 nm respectively. In addition, the dual-emission-ratio fluorescent carbon dots are high in light stability, good in water solubility, low in cytotoxicity, good in biocompatibility, capable of being used for specific detection of Fe 3+, good in selectivity and high in sensitivity; so that the fluorescent carbon dot can be used for sensitive detection of Fe 3+ in environmental water such as tap water.

Description

technical field [0001] The invention belongs to the technical field of fluorescent carbon nanomaterials, and in particular relates to a double emission ratio fluorescent carbon dot and its preparation method and application. Background technique [0002] Iron ions (Fe 3+ ) is an essential trace element in the human body, which plays an indispensable role in physiological processes, diagnosis and ecological environment. Fe 3+ Oxygen can also be carried to transport electrons and catalyze redox reactions in proteins. In environmental quality assessment, Fe 3+ Fe content is one of the important indicators to evaluate the water quality level, Fe in domestic drinking water 3+ The content shall not exceed 5.4μmol / L. In the human system, Fe 3+ A suitable total content of about 4g. In recent years, studies have reported that Fe 3+ Excessive content can cause major diseases such as coronary heart disease, cancer, methemoglobinemia and immune system dysfunction. Fe 3+ Defici...

Claims

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Application Information

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IPC IPC(8): C09K11/65C01B32/15B82Y40/00B82Y20/00G01N21/64
CPCC09K11/65C01B32/15B82Y20/00B82Y40/00G01N21/6428
Inventor 梁彩珍谢小保施庆珊王玲玲董鹏冯劲孙廷丽李素娟
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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