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A detection method for African swine fever based on quantum dot technology

A detection method, African swine fever technology, applied in measuring devices, material analysis by observing the impact on chemical indicators, instruments, etc., can solve the problems of difficult long-term preservation of results, fading of fluorescence intensity, and restrictions on popularization and application, etc., to achieve Increased accuracy and detection sensitivity, increased fluorescence photostability, time and cost savings

Inactive Publication Date: 2011-12-14
陈文刚
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Problems solved by technology

However, most of the commonly used fluoresceins are organic dyes, and there are disadvantages such as the fluorescence intensity gradually fades with time, and the results are not easy to store for a long time, which is limited in popularization and application.

Method used

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Embodiment

[0021] The detection method analyzes whether African swine fever virus exists in pig spleen and tonsil, and uses quantum dot-labeled African swine fever virus monoclonal antibody, and phosphate buffered solution (PBS) replaces the monoclonal antibody as a negative control.

[0022] The formula of borate buffer solution is:

[0023] Sodium tetraborate decahydrate 3.81g, add double distilled water to make the volume to 1000ml, pH is 8.32.

[0024] The formula of phosphate buffer solution is:

[0025] Sodium chloride 8g

[0026] Potassium chloride 0.2g

[0027] Disodium hydrogen phosphate 1.44g

[0028] Potassium dihydrogen phosphate 0.24g

[0029] Add double distilled water to 1000ml, and adjust the pH to 7.2.

[0030] Step 1, disperse the water-soluble CdTe quantum dots with carboxyl groups on the surface in borate buffer solution (PH=8.32), add diimide sulfide (EDC) and African swine fever virus monoclonal antibody, vortex to mix and oscillate evenly, React at 25°C for 3...

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Abstract

An immunohistochemical analysis and detection method based on quantum dots in the field of nanomaterials, comprising the following steps: (1) first dispersing the quantum dots in a borate buffer solution, then adding diimide sulfide and a secondary antibody, mixing , react, centrifuge, and wash to obtain the African swine fever monoclonal antibody complex labeled with quantum dots; wherein, quantum dots: diimine sulfide: the molar ratio of the secondary antibody is 60: 15: 1; or (2) first the quantum dots Disperse in a phosphate buffer solution containing 1.25% glutaraldehyde by volume, disperse, react, add a secondary antibody, react, centrifuge, and wash to obtain a quantum dot-labeled African swine fever monoclonal antibody complex; wherein, the quantum dot The molar ratio of :glutaraldehyde:secondary antibody was 60:15:1; after that, immunoreaction and observation were carried out according to the standard immunohistochemical detection method. The method of the invention directly observes with a fluorescent microscope, is simple and easy to implement, increases the stability of the fluorescent light, and improves the accuracy of analysis and the sensitivity of detection.

Description

technical field [0001] The invention relates to a detection method in the field of nanotechnology, in particular to a detection method for African swine fever based on quantum dot technology. Background technique [0002] Immunohistochemistry is the application of the basic principle of immunology - antigen-antibody reaction, that is, the principle of specific binding of antigen and antibody, which is determined by the color development of the chromogenic agent (fluorescein, enzyme, metal ion, isotope) of the labeled antibody through a chemical reaction. Tissue intracellular antigens (polypeptides and proteins) for localization, qualitative and quantitative research. Since the immune complex formed after the combination of antibody and antigen is colorless, it is also necessary to display the antigen-antibody reaction site by means of histochemical methods. Through the antigen-antibody reaction and color reaction, the chemical components in the cells or tissues can be displ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N21/78
Inventor 陈文刚
Owner 陈文刚
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