An immunohistochemical analysis and detection method based on quantum dots in the field of nanomaterials, comprising the following steps: (1) first dispersing the quantum dots in a borate buffer solution, then adding diimide sulfide and a secondary antibody, mixing , react, centrifuge, and wash to obtain the African swine fever monoclonal antibody complex labeled with quantum dots; wherein, quantum dots: diimine sulfide: the molar ratio of the secondary antibody is 60: 15: 1; or (2) first the quantum dots Disperse in a phosphate buffer solution containing 1.25% glutaraldehyde by volume, disperse, react, add a secondary antibody, react, centrifuge, and wash to obtain a quantum dot-labeled African swine fever monoclonal antibody complex; wherein, the quantum dot The molar ratio of :glutaraldehyde:secondary antibody was 60:15:1; after that, immunoreaction and observation were carried out according to the standard immunohistochemical detection method. The method of the invention directly observes with a fluorescent microscope, is simple and easy to implement, increases the stability of the fluorescent light, and improves the accuracy of analysis and the sensitivity of detection.