Preparation method for fluorescence sensing material based on molecular imprinting and carbon dots

Abstract

The invention provides a preparation method for a fluorescence sensing material based on molecular imprinting and carbon dots. The method comprises the following steps: adding a substitute template and a functional monomer into a mixed solution composed of chloroform, acetonitrile and toluene, carrying out stirring and allowing the mixed solution to fully dissolve and react, then adding the carbon dots, carrying out stirring and then adding a crosslinking agent and an initiator, carrying out ultrasound and then introducing nitrogen for water bath and incubation, carrying out filtering, carrying out placing in a vacuum drying oven for aging, carrying out washing with an extracting solvent so as to remove template molecules, and carrying out vacuum drying so as to obtain a molecular imprinting and carbon dot fluorescent sensing material. The preparation method for the fluorescence sensing material provided by the invention has the advantages of high stability, light-free bleaching, low toxicity and capability of directly forming a sensing material by embedding into molecular imprinting polymer through polymerization reaction without the need of subsequent modification; and the sensing material in the invention is simple in preparation process, and has specific recognition effect of the molecular imprinting polymer, strong fluorescence characteristic of the carbon dots, and selective recognition effect to sterigmatocystin.

Description

technical field [0001] The invention belongs to the research field of optical sensing material preparation technology and toxin detection method, and in particular relates to a preparation method of a molecular imprint-carbon dot fluorescent sensing material and its application in the identification of aspergillus versicolor toxins. Background technique [0002] Aspergillus versicolor exists widely in nature and has strong toxic effects on humans and animals. Many food crops such as barley, wheat, corn, cakes such as bean cakes, peanut cakes and common forage grass, wheat straw and straw are easily polluted by it. According to reports, corn infected with Aspergillus versicolor can produce more than 12 g / kg of Aspergillus versicolor in 21 days at 27°C. Therefore, it is imperative to increase the supervision and inspection of grains and cereals and establish appropriate and feasible detection methods for mycotoxins. [0003] Carbon dots, as a new type of carbon nanomaterial,...

AI Technical Summary

Problems solved by technology

At present, the application of carbon dot sensing materials in the field of food safety is still seldom, and there is no research on the detection of mycotoxins. The existing reports are mainly limited to the detection of heavy metal elements, and most of the detection uses There is no specific recognition effect between them, making the detection results susceptible to false positives due to the influence of other ions or matrices in the environment

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