Food-borne fluorescent nanoparticles as well as preparation method and application thereof

A fluorescent nano, food-based technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of hidden dangers in the biological safety of fluorescent nanoparticles, and achieve strong carrying capacity, high biological safety, and good cell compatibility Effect

Inactive Publication Date: 2018-07-13
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the hidden dangers in the biosafety of existing synthesized fluorescent nanoparticles, the present invention proposes a preparation method of food-source fluorescent nanoparticles and its application in the delivery of biologically active molecules, which breaks through the traditi

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  • Food-borne fluorescent nanoparticles as well as preparation method and application thereof
  • Food-borne fluorescent nanoparticles as well as preparation method and application thereof
  • Food-borne fluorescent nanoparticles as well as preparation method and application thereof

Examples

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preparation example Construction

[0045] The present invention provides a method for preparing food-derived fluorescent nanoparticles, which is characterized in that it comprises the following steps:

[0046] S1: Bake salmon or Spanish mackerel at 150~350℃ for 10~150min;

[0047] S2: The sample after baking is immersed in a polar organic solvent for extraction, and the mixed liquid after extraction is rotary evaporated to completely remove the polar organic solvent; wherein the mass to volume ratio of the sample and the polar organic solvent (g / ml) is 1:1~1:3, and the extraction time is 2~48h;

[0048] S3: Use water and non-polar organic solvent to reconstitute, separate and remove the non-polar organic solvent layer, then add non-polar organic solvent, and repeatedly extract and degrease until the water phase solution is clear and transparent; among them, water and each time are added The mass to volume ratio of the non-polar organic solvent is 1:1 to 1:3;

[0049] S4: Pass the above water-soluble extract through l...

Embodiment 1

[0064] 500g salmon (purchased from Dalian Changxing Aquatic Products Market) was roasted at 200°C for 60 minutes, and the roasted salmon was immersed in 2 times the volume of absolute ethanol for 10 hours, the supernatant was taken out, and rotary evaporated to completely remove the ethanol , Reconstitute the mixture with water: chloroform=1:1, then remove the chloroform, add chloroform for extraction and degreasing, until the aqueous phase solution is clear and transparent, the crude aqueous phase solution is concentrated and passed through D101 Porous adsorption resin column chromatography, collecting the fluorescent part, freeze-drying to obtain fluorescent nanoparticles.

[0065] TEM analysis of fluorescent nanoparticles ( figure 1 ), it can be seen that this method can successfully prepare spherical fluorescent nanoparticles with a particle size distribution of 1.6-3.6nm, an average particle size of 2.64±0.42nm, good crystallinity, and a lattice spacing of about 0.21nm . Th...

Embodiment 2

[0068] Take the fluorescent nanoparticles prepared in Example 1 and use the complete medium of liver cancer cells (Shanghai Institute of Cellular Cells, Chinese Academy of Sciences) as a solvent to prepare 10 mg / mL fluorescent nanoparticles, and use this fluorescent nanoparticle solution to culture liver cancer cells at 37°C for 5 hours , And then measure the distribution of fluorescent nanoparticles in the cell under a laser confocal microscope. The fluorescent nanoparticles are formulated into an aqueous solution and fed to model mice (SPF mice, Dalian Medical University) at a rate of 2 g / kg. After 6 hours, they are slaughtered and the intestines, brains, livers, lungs and kidneys are taken, and small animals are used. The fluorescence imager observes the distribution of fluorescent nanoparticles.

[0069] From Picture 9 It can be seen that fluorescent nanoparticles can enter the cell but not the nucleus, which indicates that the fluorescent nanoparticles have good cell compat...

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Abstract

The invention provides a preparation method of food-borne fluorescent nanoparticles. The preparation method comprises the following steps: S1: baking salmon or Spanish mackerel at 150 to 350 DEG C for10 to 150 min; S2, immersing a baked sample into a polar organic solvent and extracting; carrying out rotary evaporation on an extracted mixed solution until the polar organic solvent is completely removed, wherein the mass and volume ratio of the sample to the polar organic solvent ranges from (1 to 1) to (1 to 3) and the extracting time is 2 to 48h; S3: re-dissolving by adopting water and a non-polar organic solvent and carrying out liquid separation to remove a non-polar organic solvent layer; adding the non-polar organic solvent and repeatedly extracting and degreasing until a water-phasesolution is clarified and transparent, wherein the mass and volume ratio of the sample to the non-polar organic solvent added each time ranges from (1 to 1) to (1 to 3); enabling water-soluble extract to be subjected to liquid column chromatography for collecting a fluorescent part; carrying out freeze-drying to obtain the product. The method provided by the invention breaks through a traditionalfluorescent nanoparticle preparation manner and has the advantages that a preparation process is simple and a prepared case has high biological safety and the like.

Description

Technical field [0001] The invention relates to the technical field of preparation of novel fluorescent carbon nanomaterials and molecular transport applications, and more specifically, to a food-derived fluorescent nanoparticle, a preparation method thereof and its application in the transport of biologically active molecules. Background technique [0002] Fluorescent nanoparticles were first discovered by accident. In 2006, someone prepared and obtained carbon nanoparticles with a diameter of less than 10nm and fluorescent properties. They have a series of excellent physical and chemical properties, such as surface effects, quantum size effects, quantum tunnels Effect, good water solubility, luminescence stability, etc. Fluorescent nanoparticles have shown good application prospects in many fields such as bioimaging, biosensing, drug carriers, and photocatalysis. They have attracted widespread attention and their research has been developed rapidly. [0003] There are abundant c...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K49/00A61K49/18B82Y5/00B82Y15/00A61K31/137A61K31/704
CPCA61K31/137A61K31/704A61K49/0019A61K49/1818B82Y5/00B82Y15/00
Inventor 谭明乾宋玉昆刘珊王海涛丛爽宋勋禹李加齐曹林刘荣刚王南鹰王瑞颖谢伊莎那晓康
Owner DALIAN POLYTECHNIC UNIVERSITY
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