Preparing method for carbon quantum dots free of cytotoxicity without protection gas

A carbon quantum dot, cell-free technology, applied in the field of carbon quantum dot preparation, can solve the problems of increased production cost, uncontrollable microwave temperature, low fluorescence efficiency, etc. The effect of improving fluorescence efficiency

Inactive Publication Date: 2016-01-06
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It has been reported that fluorescent carbon dots are prepared by microwave pyrolysis of various amino acids, but there are disadvantages such as uncontrollable microwave temperature (local hot spots) and low fluorescence efficiency.
In addition, some reports on the preparation of carbon quantum dots by pyrolysis of organic matter often require the presence of protective gas, which not only increases the input of production costs, but also increases the production risk.

Method used

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  • Preparing method for carbon quantum dots free of cytotoxicity without protection gas
  • Preparing method for carbon quantum dots free of cytotoxicity without protection gas
  • Preparing method for carbon quantum dots free of cytotoxicity without protection gas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Put 2g of sodium glutamate sample (general monosodium glutamate) into a small crucible without grinding, and calcined at 450°C for 50min, with a heating rate of 10°C / min, to carry out the calcination, carbonization and doping process;

[0030] (2) Scrape off the calcined product from the crucible, grind it sufficiently, dissolve it with absolute ethanol, oscillate ultrasonically for 20 minutes, filter it with medium-speed filter paper, and dry it at 60°C for use;

[0031] (3) Dissolve the dried product in (2) with a small amount of distilled water, and dialyze with a 3000Da-3500Da dialysis membrane for 12 hours, the dialyzed fluid will be a liquid product, and a solid product will be obtained after drying.

[0032] figure 1 Flow chart of the preparation method. The products obtained in Example 1 and Comparative Example 1 were configured into 1 mg / mL carbon dot solution, and the ultraviolet absorption and fluorescence intensity were tested respectively with quinine...

Embodiment 2

[0034] (1) Put 2g of sodium glutamate sample (general monosodium glutamate) into a small crucible without grinding, the calcination time is 30min, the heating rate is 10℃ / min, no N 2 Under protection, the calcination temperature is 200°C, 300°C, 400°C and 500°C respectively, and the calcination carbonization and doping process are carried out;

[0035] (2) Scrape off the calcined product from the crucible, grind it sufficiently, dissolve it in water, oscillate ultrasonically for 20 minutes, filter it with medium-speed filter paper, and filter the filtrate again with a 0.22 μm water-based filter membrane.

[0036] Figure 5 It is a pyrolysis method to prepare inorganic non-metallic carbon particle quantum dots, the calcination time is 30min, no N 2 Under protection, the calcination temperature is 200 ℃, 300 ℃, 400 ℃ and 500 ℃ composite spectrum of ultraviolet absorption and fluorescence. It can be seen from the figure that under the same calcination time, the product whose ca...

Embodiment 3

[0038] (1) Put 2g of sodium glutamate sample (general monosodium glutamate) into a small crucible without grinding, and calcined at 400°C for 30min, with a heating rate of 10°C / min, to carry out the calcination, carbonization and doping process;

[0039] (2) Scrape off the calcined product from the crucible, grind it sufficiently, dissolve it with absolute ethanol, oscillate ultrasonically for 20 minutes, filter it with medium-speed filter paper, and dry it at 60°C for use;

[0040] (3) Dissolve the dried product in (2) with a small amount of distilled water, and dialyze with a 3000Da-3500Da dialysis membrane for 12 hours, the dialyzed fluid will be a liquid product, and a solid product will be obtained after drying.

[0041] figure 2The transmission electron microscope image of inorganic non-metallic carbon particle quantum dots prepared by pyrolysis method, the average particle size of the particles is about 6.78nm. image 3 The X-ray diffraction pattern of the inorganic n...

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Abstract

The invention relates to a preparing method for carbon quantum dots free of cytotoxicity without protection gas. The preparing method comprises the steps that sodium glutamate is calcinated at a high temperature, and carbon dot powder is obtained; carbon dots are dissolved, subjected to ultrasonic oscillation, filtered and dried; a dried product is dissolved and dialyzed, and the carbon quantum dots are obtained. The preparing method is easy and convenient to operate, free of protection of protection gas, free of high-pressure conditions and environmentally friendly, and industrial production is easy. The carbon quantum dots prepared through the method have the advantages of being free of cytotoxicity, good in biocompatibility, high in quantum efficiency, excellent in fluorescence property (fluorescence stability, dark blink and an excellent pH property), uniform in particle size distribution, good in monodispersity and the like.

Description

technical field [0001] The invention belongs to the field of preparation methods of carbon quantum dots, in particular to a preparation method of non-cytotoxic carbon quantum dots without protective gas. Background technique [0002] In recent years, due to the traditional provision of fluorescent dyes and the toxicity of traditional semiconductor quantum dots, it is difficult to apply them in actual medical marking and detection in a true sense. Even if it is applied to biomarkers and scientific research materials, the limit of addition is only just over 1 microgram per milliliter, and such a small amount of addition cannot basically meet the needs of labeling. Therefore, more and more studies have begun to focus on inorganic non-metallic carbon particle quantum dots. First, compared with traditional organic fluorescent dyes, inorganic non-metallic carbon particle quantum dots not only maintain the advantages of broad and continuous absorption spectrum of traditional metal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C01B31/02B82Y20/00
Inventor 周兴平郑楠楠丁莎
Owner DONGHUA UNIV
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