Method for detecting heavy metal based on fluorescence quenching of Cuibourtia SPP and application thereof

A technology for fluorescence quenching and heavy metals, applied in fluorescence/phosphorescence, measuring devices, material analysis through optical means, etc., can solve the problems of insufficient speed, accuracy, and low sensitivity, and achieve fast detection, improved sensitivity, and improved accuracy degree of effect

Active Publication Date: 2019-08-30
南京市产品质量监督检验院(南京市质量发展与先进技术应用研究院)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing methods for detecting heavy metals are not fast enough, accurate enough, and have low sensitivity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for detecting heavy metals based on red precious precious fluorescence quenching, comprising the following steps:

[0028] 1) Take 1g of Hongguibao wood in a 15ml centrifuge tube, add 12ml of extraction solvent into the centrifuge tube, after 24 hours of extraction, use a fluorescence spectrometer to measure the fluorescence spectrum curve of Hongguibao extract solution under λex excitation light, and obtain two emission peak lambda 1 and lambda 2 The corresponding intensities are F 1 and F 2 ;

[0029] 2) Preparation of heavy metal standard working solution: Take 0.5ml, 1ml, 2ml, 5ml and 10ml of 100μg / ml stock solution in a 100ml volumetric flask, and use 2% HNO 3 Dilute the solution to the mark and mix well to obtain standard working solutions of 0.5mg / L, 1mg / L, 2mg / L, 5mg / L and 10mg / L;

[0030] 3) Take 0.5ml of 0.5mg / L, 1mg / L, 2mg / L, 5mg / L and 10mg / L standard working solutions and mix them evenly with 10ml of the extracts from step 1), and use a fluores...

Embodiment 2

[0037] A method for detecting heavy metals based on red precious precious fluorescence quenching, comprising the following steps:

[0038] 1) Take 3g of Hongguibao wood in a 15ml centrifuge tube, add 14ml of extraction solvent into the centrifuge tube, and after 24 hours of extraction, use a fluorescence spectrometer to measure the fluorescence spectrum curve of Hongguibao extract solution under λex excitation light, and obtain two emission peak lambda 1 and lambda 2 The corresponding intensities are F 1 and F 2 ;

[0039] 2) Preparation of heavy metal standard working solution: Take 0.5ml, 1ml, 2ml, 5ml and 10ml of 100μg / ml stock solution in a 100ml volumetric flask, and use 2% HNO 3 Dilute the solution to the mark and mix well to obtain standard working solutions of 0.5mg / L, 1mg / L, 2mg / L, 5mg / L and 10mg / L;

[0040] 3) Take 0.5ml of 0.5mg / L, 1mg / L, 2mg / L, 5mg / L and 10mg / L standard working solutions and mix them evenly with 10ml of the extracts from step 1), and use a flu...

Embodiment 3

[0047] A method for detecting heavy metals based on red precious precious fluorescence quenching, comprising the following steps:

[0048] 1) Take 2g of Hongguibao wood in a 15ml centrifuge tube, add 13ml of extraction solvent into the centrifuge tube, after 24 hours of extraction, use a fluorescence spectrometer to measure the fluorescence spectrum curve of Hongguibao extract solution under λex excitation light, and obtain two emission peak lambda 1 and lambda 2 The corresponding intensities are F 1 and F 2 ;

[0049] 2) Preparation of heavy metal standard working solution: Take 0.5ml, 1ml, 2ml, 5ml and 10ml of 100μg / ml stock solution in a 100ml volumetric flask, and use 2% HNO 3 Dilute the solution to the mark and mix well to obtain standard working solutions of 0.5mg / L, 1mg / L, 2mg / L, 5mg / L and 10mg / L;

[0050] 3) Take 0.5ml of 0.5mg / L, 1mg / L, 2mg / L, 5mg / L and 10mg / L standard working solutions and mix them evenly with 10ml of the extracts from step 1), and use a fluores...

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PUM

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Abstract

The invention relates to the technical field of heavy metal detection in the environment, and in particular relates to a method for detecting heavy meal based on fluorescence quenching of Cuibourtia SPP and an application thereof. The method comprises the following steps of 1, extracting fluorescent materials of the Cuibourtia SPP by using an extraction solvent; 2, preparing a heavy metal standardworking solution; 3, measuring a fluorescence spectrum curve of the heavy metal standard working solution by using a fluorescence spectrophotometer, and thus acquiring ratio fluorescence intensity values of two fluorescence characteristic peaks; 4, fitting corresponding heavy metal concentrations with the ratio fluorescence intensity values to acquire a fitting formula; and 5, mixing a target including heavy metal with Cuibourtia SPP extracting solution, measuring the ratio fluorescence intensity value of the target by using the fluorescence spectrophotometer, putting the ratio fluorescence intensity value into the fitting formula , and thus acquiring the heavy metal concentration in the target. According to the method and the application thereof provided by the invention, after the fluorescent materials of the Cuibourtia SPP are effectively extracted, the accuracy of the heavy metal detection is significantly improved; the method provided by the invention is low in cost, simple in technology, quick to detect, and relatively high in economic benefit.

Description

technical field [0001] The invention relates to the technical field of detection of heavy metals in the environment, in particular to a method and application for detecting heavy metals based on fluorescence quenching of red precious treasures. Background technique [0002] Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds. Human activities can lead to the increase of heavy metals in the environment, beyond the normal range, and lead to the deterioration of environmental quality. Unlike the pollution of other organic compounds, heavy metals are enriched and difficult to degrade in the environment. Heavy metals in the human body will strongly interact with proteins and various enzymes, making them inactive, and may also accumulate in certain organs of the human body. If the body exceeds the tolerance limit of the human body, it will cause acute poisoning of the human body , chronic poisoning, etc. [0003] After some substanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6428G01N21/643G01N2021/6421G01N2021/6432
Inventor 袁慧雯张玉洁赵颖峰梁峙安惠芳薛涛
Owner 南京市产品质量监督检验院(南京市质量发展与先进技术应用研究院)
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