The invention discloses a multi-micro part coplane adjusting working platform and a corresponding method based on one-way microscopic vision. The platform comprises a motion platform A, a motion platform B, a microscopic vision system, a holder base, a pitching platform, a holder, micro parts and a computer. The method comprises the following steps that firstly, the microscopic vision system is adjusted to a maximum visual field, and the micro parts are adjusted into the visual field so as to form a clear image; then the image is processed to obtain the positions of the parts in the image, and the motion amount that the parts are moved to the holder in the center of the visual field is obtained through computation; finally, some micro part is moved to the center of the visual field, the vision system is adjusted to a minimum visual filed, the part is precisely focused, then the other parts are moved to the center of the visual filed in sequence, and all the parts form clear images in the vision system. The multi-micro part coplane adjusting working platform and the corresponding method based on one-way microscopic vision have the advantages that micron dimension coplane adjustment precision is realized, the method is simple and easy to carry out, and the execution efficiency is high.