Transmittance type differential confocal CARS (Coherent Anti-Stokes Raman Scattering) micro-spectrum testing method and device

Abstract

The invention belongs to the technical field of micro-spectrum imaging detection, and relates to a transmittance type differential confocal CARS (Coherent Anti-Stokes Raman Scattering) micro-spectrum testing method and a device. The key point of the method is that a CARS spectrum detection technique is combined with a differential confocal microtechnique and applied to spectrum detection and geometric measurement on transparent substances, the differential confocal microtechnique is adopted to achieve precise focus fixation and geometric measurement, and after precise focus fixation, an optimal spectrum signal of a transparent sample in a focal position of an objective lens is rapidly detected by using the CARS spectrum detection technique, then spectrum imaging of high spatial resolution is achieved, and a device for geometric morphology measurement, spectrum information measurement and atlas and spectrum integration measurement on micro transparent objects is formed. According to the method, by using the CARS spectrum detection technique, CARS spectrum information with information of a tested transparent sample can be excited within a short time (dozens of milliseconds), possibility is provided for rapidly detecting components of transparent samples, and thus the method has wide application prospects in fields such as biological medicines and material detection.

Description

technical field [0001] The invention belongs to the technical field of microspectral imaging, and relates to a transmission differential confocal CARS microspectral detection method and device, which can be used to accurately detect micro-region Raman spectra and geometric shapes of transparent samples, and obtain high spatial resolution tomogram image. technical background [0002] In 1990, the successful application of confocal Raman spectroscopy greatly improved the possibility of exploring the specific composition and morphology of tiny objects. It combines confocal microscopy technology and Raman spectroscopy technology. It has the high-resolution tomographic imaging characteristics of confocal microscopy, and has the ability of non-destructive detection and spectral analysis. It has become an important technical means for material structure measurement and analysis. , Widely used in physics, chemistry, biomedicine, material science, petrochemical, food, medicine, crim...

AI Technical Summary

Problems solved by technology

[0008] In addition, most spectral detection techniques and devices are based on reflective structures, which are not effective when detecting transparent materials

The above reasons limit the ability of the CARS microscope system to detect micro-region spectra, and restrict its application in the measurement and analysis of micro-region spectra of transparent samples

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