Microscopical hyperspectral chromatography three-dimensional imaging device

Abstract

The invention discloses a microscopical hyperspectral chromatography three-dimensional imaging device, which comprises a light source unit, a three-dimensional scanning platform, a front microscopical light path unit, a beam-splitting imaging unit, a signal pretreatment unit, a signal acquisition unit and a control unit. The device integrates a hyperspectral imaging technology, a microtechnique, a fluorescence molecular tomography and a three-dimensional imaging technology. The device can obtain the two-dimensional hyperspectral chromatography images under conditions of multiple wavelengths and angles of an object and can obtain the light spectrum under wavelength corresponding to each pixel; meanwhile, based on a three-dimensional image reconstruction technology, the device can obtain the three-dimensional microscopical hyperspectral image of the object; and combining with the microtechnique, the device can obtain the micro three-dimensional hyperspectral chromatography images of the surface and internal structures of micro objects. Compared with other spectrographs, the device has the advantages of more information, higher precision, more powerful functions and higher stability. The device is suitable for biomedical test, food safety test and ecological environment monitoring.

Description

technical field [0001] The invention is applicable to the fields of biomedical detection, food safety detection and ecological environment monitoring, etc., and relates to a micro hyperspectral tomography three-dimensional imaging device. Background technique [0002] Imaging spectroscopy technology originated from the multispectral remote sensing technology in the 1970s, and has developed into a powerful tool in the field of remote sensing applications since the mid-to-late 1980s. With the improvement of multispectral resolution, it has now developed into hyperspectral imaging technology, and Subsequently, it is applied in fields such as biomedicine, food safety, environmental testing and military affairs. The hyperspectral imaging technology is a newly developed imaging spectrum technology. It has higher spectral and spatial resolution than the previous multispectral imaging and hyperspectral imaging technologies, and the amount of data information obtained is higher than ...

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Problems solved by technology

Chinese utility model patent specifications (notification numbers CN 2401883Y and CN 2651731Y and CN 2457581Y) disclose three kinds of tomographic imaging devices, but these devices do not involve microscopic imaging technology, and the structure is complex and the system stability is poor

Chinese Invention Patent Application Publications (CN 101526477A and CN 1369702A) respectively disclose a laser differential confocal spectrum micro-tomography device and a high-speed optical interference tomography device, but these devices have complex structures and only The micro-tomography imaging of Raman spectrum or interference spectrum is not yet able to perform three-dimensional micro-tomography imaging of the measured object, and the scope of application is limited

Microscopic technology has been widely used in the microscopic biomedical field. Through microscopic technology, things and their shapes that cannot be seen by the naked eye can be observed. However, the current medical microscopic technology mainly makes small pieces of the measured substance first. Slicing, and then placing it on the platform for microscopic observation, this traditional microslicing technique is destructive, and cannot realize microtomography imaging of large objects without damage in vivo, such as: Chinese Invention Patent Application Publication (Publication No. CN 101477241A) discloses a micro-optical scanning tomography device, which is to perform destructive slices on objects first, and then perform optical tomography

At present, spectral imaging or tomographic imaging technology mostly stays in two-dimensional imaging, but because the wavelength of the excitation light source is different, the penetration depth of the excitation light to the measured object is also different, and the detection limitations of deeper substances will be limited. As a result, it is impossible to identify all the information of the measured object, so that the application of hyperspectral two-dimensional imaging is limited, and the application of three-dimensional imaging technology to micro hyperspectral tomography will be able to obtain the microscopic hyperspectral tomography of the measured object. Three-dimensional scene, and can make a satisfactory interpretation of the properties of the measured object

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