Speckle blood flow imaging method and device based on component analysis

A component analysis and imaging method technology, applied in the field of biological tissue optical blood flow imaging, can solve the problems of being easily affected by image jitter, low image temporal resolution, low image spatial resolution, etc. The effect of high resolution and high imaging accuracy

CN107485383AActive Publication Date: 2017-12-19佳维斯(武汉)生物医药有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2017-12-19

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Abstract

The invention discloses a speckle blood flow imaging method and device based on component analysis. The method comprises the steps of performing component analysis on an original blood flow speckle signal, separating out a blood flow light intensity signal and a tissue light intensity signal, calculating out an imaging parameter, utilizing the imaging parameter as a gray value of a two-dimensional blood flow distribution diagram and establishing a two-dimensional blood flow distribution diagram of a sample. The method has the advantages of no invasion, no contrast medium injection, no damage and the like; the method can be applied to living small animal samples like blood flow imaging of mice, fish embryo and the like; the method has great application prospect in pathology diagnosis and analysis of a part of blood flow diseases. The device has the advantages of low cost, no damage to samples, ability in quickly achieving blood flow imaging, high imaging accuracy and application to blood flow monitoring; a transmission type imaging system is utilized, so that a deeper imaging depth is obtained; a long-focus telecentric lens is introduced into, so that blood flow at a deep position is in a focus depth range, a camera can collect clear images, and a higher spatial resolution is obtained.
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Description

technical field

[0001] The invention relates to the field of optical blood flow imaging of biological tissues, in particular to a speckle blood flow imaging method and device based on component analysis. Background technique

[0002] Laser speckle blood flow imaging belongs to the field of optical imaging technology. It obtains blood flow information by collecting speckle signals of biological tissues. It can obtain the blood flow distribution map of the whole field without injection of contrast agent and mechanical scanning. It has non-destructive and non-contact imaging advantages. At present, laser speckle blood flow imaging technology has been successfully applied to blood flow imaging and monitoring of cerebral cortex, skin, eye retina and other tissues, which is of great significance for the pathological diagnosis and analysis of some blood flow diseases.

[0003] The speckle blood flow imaging method usually continuously collects N frames of speckle images with a siz...

Examples

Embodiment Construction

[0040] refer to figure 1 , a speckle blood flow imaging method based on component analysis of the present invention, comprising the following steps:

[0041] The laser is irradiated to the sample to be tested, and the original blood flow speckle signal is transmitted;

[0042] Collect the original blood flow speckle signals including blood flow light intensity signals and tissue light intensity signals, and arrange them in time series;

[0043] The components of the original blood flow speckle signal are analyzed to obtain the blood flow light intensity signal produced by the moving red blood cells and the tissue light intensity signal produced by the background tissue;

[0044]Calculate the imaging parameters of each pixel of the blood flow profile;

[0045] Using the above steps, the imaging parameters of each row of pixels are sequentially obtained, and used as the gray value of the two-dimensional blood flow distribution map to establish a two-dimensional blood flow dist...