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Compressed sensing multi-photon body imaging device and method and optical system

A technology of compressed sensing and imaging devices, which is applied in the field of computational imaging and life sciences, can solve the problem that the imaging resolution of the microscopic system and the size of the field of view are difficult to achieve high performance, and achieve the goal of increasing imaging depth and improving collection efficiency Effect

Pending Publication Date: 2020-02-21
北京超纳视觉科技有限公司
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Problems solved by technology

[0005] Moreover, limited by the size of the objective lens, the imaging resolution and field of view of the microscope system are usually difficult to achieve high performance.

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  • Compressed sensing multi-photon body imaging device and method and optical system
  • Compressed sensing multi-photon body imaging device and method and optical system
  • Compressed sensing multi-photon body imaging device and method and optical system

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Embodiment Construction

[0037] In order to make the content of the present invention clearer and easier to understand, the content of the present invention will be described in detail below with reference to specific embodiments and drawings.

[0038] figure 1 It schematically shows a system block diagram of a compressed sensing multiphoton volume imaging device according to a preferred embodiment of the present invention.

[0039] Such as figure 1 As shown, the system of a compressed sensing multiphoton volume imaging device according to a preferred embodiment of the present invention includes: a point scanning excitation device 101, a multi-angle excitation device 102, a compressed sensing module 103, an optical illumination device 104, a signal acquisition device 105, and an image reconstruction device. 结构module 106.

[0040] Among them, the point scanning excitation device 101 is used to realize laser scanning excitation on the imaging surface. Since the excitation end is not fully filled with the back f...

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Abstract

The invention provides a compressed sensing multi-photon body imaging device and method and an optical system. The optical system comprises a photomultiplier, a first convergent lens, a filter, a first beam expander, a dichroscope, a second beam expander, an objective lens, a first galvanometer, a first collimating lens, a second galvanometer, a second convergent lens, a second collimating lens, athird convergent lens and a femtosecond laser. An exciting light path in the light path comprises the third convergent lens, the second collimating lens, the second convergent lens, the second galvanometer, the first collimating lens, the first galvanometer, the second beam expander, the second dichroscope and the objective lens which sequentially pass through the femtosecond laser to reach a sample; a detection light path in the light path sequentially passes through the objective lens, the second dichroscope, the first beam expander, the filter and the first convergent lens from the sampleto the photomultiplier.

Description

Technical field [0001] The present invention relates to the field of computational imaging, in particular to the field of life sciences; more specifically, the present invention relates to a wide-field, high-resolution, and high-speed compressed sensing multiphoton volume imaging device and method. Background technique [0002] Multiphoton microscope has become an indispensable tool in the field of life sciences. It is well-known for its natural tomographic capability and high penetration depth. At the same time, the multiphoton microscope has the advantages of multi-modal imaging. It can collect multiple types of signals, such as fluorescent label imaging, autofluorescence imaging, and second and third harmonic imaging, to provide more sample information. Multiphoton microscopes are developing in four directions: fast imaging speed, large imaging area, high imaging resolution and deep imaging depth. However, it is very difficult for a multiphoton microscope to obtain high perfo...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6458
Inventor 吴嘉敏赵志锋肖红江
Owner 北京超纳视觉科技有限公司
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