Tissue optical clearing agent and application thereof

A light-transparent agent and tissue technology, applied in the field of bio-optics, can solve the problems of affecting the second harmonic imaging of collagen, the destruction of collagen space structure, and the complex composition of light-transparent agent, so as to achieve uniform refractive index and endogenous The effect of strong optical signal and short time-consuming

Active Publication Date: 2015-04-29
SHENZHEN INST OF ADVANCED TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the existing technology provides a variety of tissue light transparent agents, these technologies are mainly based on the basic principle of collagen dissociation, which will lead to the destruction of the spatial structure of collagen and the weakening or even disappearance of endo

Method used

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  • Tissue optical clearing agent and application thereof
  • Tissue optical clearing agent and application thereof
  • Tissue optical clearing agent and application thereof

Examples

Experimental program
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Example Embodiment

[0035] Example 1

[0036] SD rats (200g) were intraperitoneally injected with 1% pentobarbital 2ml and then sacrificed by cervical dislocation. The rat’s hair was removed with depilatory cream, the rat’s skin was washed with saline, and the back skin was removed with scissors. The knife removes the subcutaneous fat. Wash the rat skin with phosphate buffer and soak it in 30% fructose, 10% polyethylene glycol monooctyl phenyl ether (Sigma-Aldrich reagent, n=9-10) and 25% respectively. In an aqueous solution of transparent agent composed of N,N,N',N'-tetrakis(2-hydroxypropyl)ethylenediamine, the skin becomes transparent after 15-20 minutes. The transparent skin is flattened, and a two-photon fluorescence microscope is used to obtain the second harmonic signal diagram of the skin at a depth of about 120 μm (where the excitation wavelength is 900 nm, and the receiving wavelength is 360 ~ 540 nm). Figure 1A . Use a two-photon fluorescence microscope to obtain the fluorescence signal ...

Example Embodiment

[0039] Example 2

[0040] SD rats (200g) were intraperitoneally injected with 2ml of 1% pentobarbital and sacrificed by cervical dislocation. The eyeballs were carefully taken out with tweezers and scissors. After washing the remaining blood on the eyeballs with phosphate buffer, they were placed in 4% The polyoxymethylene was fixed for 24 hours, and then washed with phosphate buffer after removal. Soak the prepared eyeballs in 70% fructose, 2% polyethylene glycol monooctyl phenyl ether (Sigma-Aldrich reagent, n=9-10) and 5% N, N, N'respectively. ,N'-Tetra(2-hydroxypropyl)ethylenediamine in an aqueous transparent agent solution, the eyeball becomes transparent after 15-20 minutes, such as figure 2 Shown, where figure 2 The left picture is the eyeball before treatment, and the right picture is the transparent eyeball after treatment. Use a two-photon fluorescence microscope to obtain the second harmonic signal diagram of the eyeball at different depths (the excitation wavelengt...

Example Embodiment

[0041] Example 3

[0042] SD rats (200g) were intraperitoneally injected with 2ml of 1% pentobarbital and sacrificed by cervical dislocation. The eyeballs were carefully taken out with tweezers and scissors. After washing the remaining blood on the eyeballs with phosphate buffer, they were placed in 4% The polyoxymethylene was fixed for 24 hours, and then washed with phosphate buffer after removal. Soak the prepared eyeballs in 50% fructose, 5% polyethylene glycol monooctylphenyl ether (Sigma-Aldrich reagent, n=9-10) and 10% N, N, N' ,N'-Tetra(2-hydroxypropyl)ethylenediamine in an aqueous solution of a transparent agent, the eyeball becomes transparent after 15-20 minutes. Use a two-photon fluorescence microscope to obtain the second harmonic signal of the eyeball at a depth of 170μm Figure (where the excitation wavelength is 900nm and the receiving wavelength is 360~540nm), we get Figure 4 . From Figure 4 It can be seen that the second harmonic signal of the eyeball at 170...

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Abstract

The invention provides a tissue optical clearing agent and an application thereof. The tissue optical clearing agent comprises 30-70 wt% of fructose, 2-10 wt% of octyl phenoxy poly ethoxy, 5-25 wt% of N,N,N',N'-tetra (2-hydroxypropyl) ethylene diamine and the balance of water. The tissue optical clearing agent does not damage the spatial structure of collagen in tissue, a secondary harmonic signal diagram or a fluorescent signal diagram of collagen of the tissue can be obtained, and the tissue optical clearing agent can greatly increase an imaging depth.

Description

technical field [0001] The invention relates to a tissue light-transparency agent and an application thereof, in particular to a tissue light-transparency agent capable of maintaining the spatial structure of tissue collagen and an application thereof, belonging to the field of bio-optics. Background technique [0002] With the development of modern optical imaging technology and the emergence of various labeling technologies, it is possible to obtain high-resolution information on the structure and function of biological tissues, but the high scattering characteristics of biological tissues seriously restrict the application of optical imaging technology in the field of biomedicine. Multiphoton microscopy imaging technology uses near-infrared light to excite chromophores to obtain fluorescence or harmonic signals to improve the imaging depth. However, for biological tissues with large scattering, the penetration depth is still very limited. [0003] The optical transparency...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N21/64
Inventor 郑炜张洋
Owner SHENZHEN INST OF ADVANCED TECH
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