Tissue optical clearing agent, preparation method thereof and application

Abstract

The invention provides a tissue optical clearing agent which comprises, in weight percent, 15-25% of urea, 8-15% of formamide, 10-20% of triton, 20-25% of N,N,N',N'-Four (2-hydroxy propyl) ethylenediamine and the rest water. According to the tissue optical clearing agent, biological tissue scattering can be effectively reduced in a short time, tissue transparency is achieved, the integrity of biological tissues can be effectively kept, the volume of the biological tissues is basically unchanged, expansion or contraction is avoided, tissues cannot become brittle, so that imaging depth and signal-to-noise ratio are greatly improved, and endogenous fluorescent signal strength is basically unaffected. The invention further provides a preparation method and an application of the tissue optical clearing agent.

Description

technical field [0001] The invention relates to the technical field of biomedicine and transparent sample preparation, in particular to a tissue light transparent agent and its preparation method and application. Background technique [0002] With the continuous development of optical imaging technology, it has become possible to obtain high-resolution structure and function information of biological tissue, but the opacity of biological tissue restricts the application of optical imaging technology in the field of biomedicine. [0003] The tissue light-transparency technology that has emerged in recent years allows organs to be observed and studied in a complete state without slicing. The core of this technology is that the introduction of hypertonic and high-refractive chemical reagents into biological tissues can effectively reduce the scattering characteristics of biological tissues. Make the sample transparent, greatly improving the depth of imaging. The tissue transpa...

AI Technical Summary

Problems solved by technology

The tissue transparent technologies used in the prior art include BABB technology, Scale technology, SeeDB technology, CLARITY technology, etc. Among them, the BABB technology uses organic solvents. Although the tissue transparent effect is good after treatment, the tissue shrinks severely when it is processed, and causes fluorescence in the tissue. The marker signal is quenched; the Scale technology is to continuously soak the biological tissue in the urea solution, which takes a long time to make the tissue transparent, and will also cause serious swelling of the tissue; the SeeDB technology uses the fructose solution with increasing concentration to soak the tissue, and the tissue shape Although it is well maintained, the tissue often turns brown, and the fructose solution is viscous, which makes it inconvenient to operate; CLARITY technology uses electrophoresis to remove the lipids in the tissue that cause scattering to achieve transparency. This method has a good effect on tissue transparency, but it needs to be The tissue is pre-fixed with a special hydrogel, and the fat-dissolving will cause the destruction of the cell membrane and the disappearance of the natural texture of the tissue

[0004] The tissue light transparency schemes reported above will more or less change the shape of the sample, destroy the natural texture structure of the tissue, damage the optical signal, and affect the imaging results

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