Culture medium for human-derived T lymphocytes and preparation method and application thereof

A lymphocyte and culture medium technology, which is applied in the field of human T lymphocyte culture medium and its preparation, can solve the problems of weak tumor killing function and slow T lymphocyte expansion speed, achieve good tumor killing function and enhance killing Activity, activity-enhancing effect

Active Publication Date: 2017-09-12
SHENZHEN BINDEBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the object of the present invention is to provide a culture medium of human-derived T lymphocytes and its preparation method and application, aiming at solving the problem of slow expansion of antigen-specific T lymphocytes after genetic modification, tumor The problem of weak lethality

Method used

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  • Culture medium for human-derived T lymphocytes and preparation method and application thereof
  • Culture medium for human-derived T lymphocytes and preparation method and application thereof
  • Culture medium for human-derived T lymphocytes and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0066] A medium for cultivating and promoting the high-efficiency expansion of human-derived T lymphocytes, wherein each 1L of the medium includes the following components:

[0067] Human AB serum 100mL;

[0068] MEM vitamin 10mL;

[0069] N-acetyl-L-cysteine ​​10mM;

[0070] L-alanyl-L-glutamine (GlutaMax) 2mM;

[0071] Sodium pyruvate 1mM;

[0072] Sodium 4-hydroxyethylpiperazineethanesulfonate (HEPES sodium salt) 20mM;

[0073] IL-7 5 μg;

[0074] IL-15 heterodimer 7.5 μg;

[0075] X-VIVO basic medium 848mL.

[0076] Wherein, the above medium is prepared by the following method: N-acetyl-L-cysteine ​​is dissolved in X-VIVO basic medium to obtain N-acetyl-L-cysteine ​​with a concentration of 1M solution; L-alanyl-L-glutamine (GlutaMax) was dissolved in X-VIVO basic medium to obtain a GlutaMax solution with a concentration of 0.2M; sodium pyruvate was dissolved in X-VIVO basic medium, Obtain a sodium pyruvate solution with a concentration of 0.1M; dissolve 4-hydroxyet...

Embodiment 2

[0079] A medium for cultivating and promoting the high-efficiency expansion of human-derived T lymphocytes, wherein each 1L of the medium includes the following components:

[0080]

[0081] The preparation method of the culture medium described in this example is similar to that of Example 1, and will not be described here again.

Embodiment 3

[0083] A medium for cultivating and promoting the high-efficiency expansion of human-derived T lymphocytes, wherein each 1L of the medium includes the following components:

[0084]

[0085]

[0086] The preparation method of the culture medium described in this example is similar to that of Example 1, and will not be described here again.

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Abstract

The invention provides a culture medium for human-derived T lymphocytes. Each 1L of the culture medium comprises the following components: 80-120 mL of human AB serum, 7-15 mL of an MEM vitamin solution, 7-12 mM of N-acetyl-L-cysteine, 1.6-2.5 mM of glutamine or a derivative, 0.8-1.2 mM of sodium pyruvate, 15-25 mM of 4-hydroxyethyl piperazine sodium esilate, 4-6 mu g of IL-7, 6-9 mu g of IL-15 or heterodimer thereof, and the balance of X-VIVO basic culture medium, wherein the glutamine or the derivative thereof comprises glutamine or L-alanyl-L-glutamine. The culture medium can be used for culturing antigen specific T lymphocytes, the cell proliferation rate of the antigen specific T lymphocytes can be increased, and the tumor killing ability can be high. The invention further provides a preparation method and application of the culture medium.

Description

technical field [0001] The invention relates to the field of biological cell culture, in particular to a culture medium of human T lymphocytes and a preparation method and application thereof. Background technique [0002] Cancer has always been a huge problem plaguing mankind. Although surgery, chemotherapy, radiotherapy and other treatments can greatly improve the survival rate of cancer patients, these treatments are far from enough to save tens of millions of cancer patients around the world. With the rapid development of biotechnology, tumor immune cell therapy has become the fourth largest therapy in the field of cancer treatment. It can make up for the shortcomings of traditional therapy and is considered to be the most promising treatment in the comprehensive tumor treatment model in the 21st century. It uses biotechnology and biological agents to separate immune cells from patients, culture, activate and expand them in vitro, and then infuse them back into patients...

Claims

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Application Information

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IPC IPC(8): C12N5/0783
CPCC12N5/0636C12N2500/30C12N2500/32C12N2500/38C12N2501/2307C12N2501/2315C12N2509/00
Inventor 张长风
Owner SHENZHEN BINDEBIOTECH CO LTD
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