Multi-photon ultra-deep tissue imaging equipment based on coherent detection and detection imaging method thereof

Abstract

The invention discloses multi-photon ultra-deep tissue imaging equipment based on coherent detection and a detection imaging method thereof. A multi-photon imaging technology is combined with an optical coherence tomography technology to realize ultra-deep and high-molecular specific imaging of a detected sample. The equipment comprises an ultrashort pulse femtosecond laser light source, a light modulation unit and an optical coherence microscopic unit. The light modulation unit modulates input laser to generate continuous spectrum laser. The optical coherence microscopic unit is used for conducting coherent detection on a biological sample. The system can detect OCT and SH-OCT signals of a tissue at the same time and obtain the specific structure of the tissue and the molecular micro-environment information of the surrounding environment of the tissue, and can quantitatively characterize the structure and function of the tissue through an image analysis method. The equipment can reflect the ultra-deep structure and functional information of the tissue, has the advantages of ultra depth, strong molecular specificity recognition capability, high temporal-spatial resolution, rapid imaging and the like, and provides the tissue structure and function information and the molecular specificity information at the same time.

Description

technical field [0001] The invention belongs to the technical field of optical microscopic imaging, and in particular relates to a coherent detection-based multiphoton ultra-deep tissue imaging device and a detection and imaging method thereof. Background technique [0002] Large-depth imaging of biological tissues can help us detect various pathological changes in tissues more comprehensively. For example, in human skin, dysfunctional cells at the bottom of the epidermis are the main cause of skin cancers such as melanoma, basal cell carcinoma, squamous cell carcinoma, etc. These cells infiltrate into the deeper dermis of the skin in the second stage of the cancer, Finally, it reaches the subcutaneous fat. Studies have shown that if cancer is detected at an early stage and treated in time, the survival rate can be as high as 97%. High-resolution imaging of the epidermis and dermis of the skin is helpful for early detection and real-time monitoring of cancer cell metastasi...

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Problems solved by technology

Because the contrast of optical coherence tomography comes from the change of biological refractive index, but because the refractive index change

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