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Broadband spectrum fluorescent imaging equipment

A fluorescence imaging, wide-spectrum technology, used in fluorescence/phosphorescence, material analysis by optical means, measurement devices, etc., can solve biological autofluorescence interference, difficult to achieve high penetration depth of biological tissue and high spatial resolution imaging, etc. problem, to avoid the effect of time synchronization

Pending Publication Date: 2017-06-13
苏州影睿光学科技有限公司
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

The working spectrum range of traditional fluorescence imaging is mainly in the visible light (400-650nm) and near-infrared region (NIR-I 650-950 nm). Influenced by absorption and scattering, but also severely interfered by biological autofluorescence, it is difficult to achieve high penetration depth and high spatial resolution imaging of biological tissues

Method used

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  • Broadband spectrum fluorescent imaging equipment

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Embodiment 1

[0036] The following examples illustrate the working process of the fluorescence imaging system:

[0037] In this embodiment, a nude mouse is used as a model as the object to be imaged, and graphene quantum dots with an emission wavelength of 1050 nm are selected as the fluorescent substance. First turn on the power switch of the wide-spectrum imaging device; then select a laser with an excitation wavelength of 785 nm and a long-pass filter of 1000 nm on the controller; inject anesthesia needles into the mice from the tail vein, wait for the mice to be anesthetized before Inject 50 μL of 1mg / mL graphene quantum dots; place the mouse on the stage, adjust the beam expander and the stage through the controller to make it to a suitable imaging position; then pass the focusing objective lens Adjust the focus to make the image clear; further, adjust the power of the laser to 10W, the imaging mode of the imaging camera, and the exposure time to 1000 ms through the controller, so that...

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Abstract

The invention discloses broadband spectrum fluorescent imaging equipment which is mainly composed of an excitation light source, an imaging camera, a controller, an optical fiber unit, a focusing objective lens, a beam expander, an objective table and the like. The excitation light source, the imaging camera, the optical fiber unit, the focusing objective lens, the beam expander, and the objective table can be controlled by the controller. The fluorescent imaging wavelength coverage of the broadband spectrum fluorescent imaging equipment is 400-1700nm, can perform fluorescent imaging on a to-be-imaged object to obtain a fluorescent imaging picture, and particularly can efficiently and rapidly perform nondestructive deep tissue imaging on a living small animal without damage.

Description

technical field [0001] The invention relates to a fluorescent imaging device, in particular to a wide-spectrum fluorescent imaging device with a wavelength range of visible light-near infrared first region-near infrared second region. Background technique [0002] Fluorescence imaging technology is widely used in biomedical research because of its fast imaging speed, high sensitivity, and low economic cost. It has broad application prospects in the large-scale screening of drugs and the evaluation of the therapeutic effect of drugs on diseases. Existing methods including fluorescence intensity analysis, fluorescence polarization detection, fluorescence resonance energy transfer detection, and fluorescent reporter systems are widely used in high-throughput drug screening. The working spectrum range of traditional fluorescence imaging is mainly in the visible light (400-650nm) and near-infrared region (NIR-I 650-950 nm). Due to the influence of absorption and scattering, and...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/01G01N21/64G01N21/6402G01N21/645G01N21/6456G01N21/6486G01N2021/6471G01N2021/6423G01N2021/0112G01N2201/06113
Inventor 孙国凤刘莉丽
Owner 苏州影睿光学科技有限公司
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