Broadband spectrum fluorescent imaging equipment
A fluorescence imaging, wide-spectrum technology, used in fluorescence/phosphorescence, material analysis by optical means, measurement devices, etc., can solve biological autofluorescence interference, difficult to achieve high penetration depth of biological tissue and high spatial resolution imaging, etc. problem, to avoid the effect of time synchronization
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[0036] The following examples illustrate the working process of the fluorescence imaging system:
[0037] In this embodiment, a nude mouse is used as a model as the object to be imaged, and graphene quantum dots with an emission wavelength of 1050 nm are selected as the fluorescent substance. First turn on the power switch of the wide-spectrum imaging device; then select a laser with an excitation wavelength of 785 nm and a long-pass filter of 1000 nm on the controller; inject anesthesia needles into the mice from the tail vein, wait for the mice to be anesthetized before Inject 50 μL of 1mg / mL graphene quantum dots; place the mouse on the stage, adjust the beam expander and the stage through the controller to make it to a suitable imaging position; then pass the focusing objective lens Adjust the focus to make the image clear; further, adjust the power of the laser to 10W, the imaging mode of the imaging camera, and the exposure time to 1000 ms through the controller, so that...
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