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A kind of preparation method and product of fluorescent mulberry silk under near-infrared light

A technology of near-infrared light and mulberry silk, which is applied in chemical instruments and methods, luminescent materials, and the molding or processing of animal feed, etc. It can solve the problems of low fluorescence specificity, weak penetration of excitation light, and difficulties in imaging and detection applications, etc. problems, achieve high biological safety, wide application range, and improve the effect of fluorescence

Active Publication Date: 2020-06-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the defects of low fluorescence specificity, weak excitation light penetration, and difficult imaging and detection applications in the existing technology for preparing fluorescent silk, the present invention provides a preparation method for preparing mulberry silk that is excited by near-infrared light and fluoresces. This method can not only improve fluorescent silk fibers with high fluorescence intensity, strong penetrating power and good biocompatibility, but also is suitable for mass production and has no pollution to the environment

Method used

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  • A kind of preparation method and product of fluorescent mulberry silk under near-infrared light
  • A kind of preparation method and product of fluorescent mulberry silk under near-infrared light

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Prepare green fluorescent core-shell structure upconversion nanoparticles (β-NaYF4:Yb,Er@β-NaYF4) by hydrothermal method, with a diameter of about 50nm, and graft the surface with polyacrylic acid (PAA) after modification Legumin, as a raw material for food addition, is synthesized as follows:

[0042] The core-shell upconversion nanoparticles (β-NaYF 4 :Yb,Er@β-NaYF 4 ), which were dispersed in n-hexane solution. The surface of nanoparticles was grafted with polyacrylic acid (PAA) by ligand exchange method. 1 mL of upconverting nanoparticles solution was mixed with 5 mL of dimethylformamide (DMF), and then 4 mL of n-hexane was added and stirred for 3 hours. Add 5-10 mL of isopropanol to precipitate and wash with DMF several times. Resuspend the precipitate in PAA / DMF solution with a PAA concentration of 10mg / mL (5-10mL), stir overnight and wash by centrifugation. Take 10mg of PAA-modified nanoparticles and disperse them in 1mL aqueous solution, add 1mg each of ...

Embodiment 2

[0049] (1) Upconversion nanoparticles (NaYF 4 : 1% Er, 1% Tm), with a diameter of about 60nm, the surface is modified with polyacrylic acid and grafted with concanavalin as a raw material for food addition. The synthesis method is as follows:

[0050] Red fluorescent upconversion nanoparticles (NaYF 4 : 1% Er, 1% Tm), which was dispersed in n-hexane solution. The surface of nanoparticles was grafted with polyacrylic acid (PAA) by ligand exchange method. 1 mL of upconverting nanoparticles solution was mixed with 5 mL of dimethylformamide (DMF), and then 4 mL of n-hexane was added and stirred for 3 hours. Add 5-10 mL of isopropanol to precipitate and wash with DMF several times. Resuspend the precipitate in PAA / DMF solution with a PAA concentration of 10mg / mL (5-10mL), stir overnight and wash by centrifugation. Take 10mg of PAA-modified nanoparticles and disperse them in 1mL of aqueous solution, add 1mg of EDC and NHS respectively, stir for 3-6h, then stir 30uL of concanavali...

Embodiment 3

[0056] (1) Prepare green fluorescent core-shell structure upconversion nanoparticles (NaGdF4:Yb / Er@NaGdF4:Yb / Nd) by hydrothermal method, with a diameter of about 50nm, and graft concanavalin after surface polyacrylic acid modification , as a raw material for adding food;

[0057] (2) disperse the up-converting nanoparticles synthesized in step (1) with water, and prepare a dispersion solution whose concentration is 5g / L;

[0058] (3) Extract mature mulberry leaves, soak them in the nanoparticle solution of step (2), take them out after soaking for 5 minutes, and let them dry naturally.

[0059] (4) On the third day of the 5th instar silkworm, feed the silkworm the treated mulberry leaves in step (3) until it spins silk and cocoons.

[0060] (5) After the silkworm spins and cocoons, silkworm cocoons are taken for silk reeling to obtain green fluorescent mulberry silk excited by near-infrared light, and the fluorescence intensity is higher.

[0061] The mulberry silk prepared ...

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Abstract

The invention discloses a method for preparing mulberry silk that fluoresces under near-infrared light, comprising: (1) preparing upconversion nanoparticles, and performing surface modification with concanavalin to obtain the modified upconversion nanoparticles; 2) Uniformly disperse the up-conversion nanoparticles in water, and prepare an aqueous solution with a concentration of up-conversion nanoparticles; (3) Extract mature mulberry leaves, immerse the mulberry leaves in the nano-particle aqueous solution system, drain the water and dry naturally (4) After the silkworm grows to the set time, the silkworm is fed with treated mulberry leaves until the silkworm spins and cocoons; (5) Collects silk to obtain fluorescent mulberry silk under near-infrared light. The present invention selects up-conversion nanoparticles capable of emitting fluorescence under the irradiation of near-infrared light with stronger penetrating power, and has better application for deep tissue imaging. Moreover, the properties of the upconversion nanoparticles are more stable, the biological safety is higher, the signal-to-noise ratio is stronger, and the scope of application is wider.

Description

technical field [0001] The invention belongs to the field of silk fiber production and processing, and in particular relates to a method for preparing mulberry silk that fluoresces under near-infrared light. Background technique [0002] Silk cellulose has the reputation of "fiber queen". It has excellent mechanical properties, and the formed fabric is smooth, bright in color, and has good air permeability and heat insulation. Therefore, silk fiber has played an important role in the course of my country's economic development. However, with the rapid development of man-made fiber industrial synthesis technology, the traditional sericulture textile industry is facing severe challenges. In recent years, research on the modification of natural silk has received more and more attention. Researchers have introduced or modified functional macromolecules into silk fibers by different means to improve the performance of silk fibers and enhance their application value. At present,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/04A23K50/90A23K20/105A23K20/147C09K11/02C09K11/85
CPCA01K67/04A23K50/90A23K20/105A23K20/147C09K11/025C09K11/7773A23K10/30A23K20/22A23K40/00B82Y5/00B82Y30/00C09K11/02B82Y99/00C09K11/7766
Inventor 杨明英王捷陈玉银张颖
Owner ZHEJIANG UNIV
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