32 results about "Legumin" patented technology

The invention discloses an application of a soybean ZF-HD protein coding gene GmZFHD11 in the aspect of influencing the quality of soybean seeds. The nucleotide sequence of the soybean ZF-HD protein coding gene GmZFHD11 is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the soybean ZF-HD protein coding The constructed plant overexpression vector pBA002-GmZFHD11 is subjected to agrobacterium tumefaciens-mediated soybean genetic transformation to obtain a soybean receptor variety Jack, and it is found that compared with a receptor control group, the content of sulfur-containing amino acid in seeds is increased, the content of protein is increased, the content of beta-conglycinin (7S) is reduced, the content of glycinin (11S) is increased, and the 11S / 7S ratio is increased; and the quality of the overexpressed transgenic soybean strain seed protein is improved. Therefore, the gene can be introduced into plants as a target gene to improve the quality of soybean grain protein.

The invention belongs to the field of food processing, and discloses a preparation method and application of a targeted colon delivery polyunsaturated fatty acid grease microcapsule. 11S globulin in soybeans and Arabic gum are used as wall materials of the microcapsule, complex coacervation of the 11S globulin in the soybeans and the Arabic gum is induced under a certain pH condition, so that oil drops are wetted by a coacervate, a core-shell structure is formed, a coacervate phase is further stabilized through glucan to inhibit coalescence, and on the basis, the microcapsule is prepared. And tannic acid, polysaccharide and protein can form hydrogen bonds and generate hydrophobic interaction to further crosslink the condensed phase to obtain the microcapsule capable of resisting digestion of the stomach and the small intestine, so that colon delivery of polyunsaturated fatty acid is realized.

The invention discloses functional recombinant soybean protein isolate emulsion gel as well as a preparation method and application thereof, and belongs to the technical field of soybean protein processing. The preparation method comprises the following steps: recombining three main components, namely soybean lipophilic protein, glycinin and beta-conglycinin, in soybean protein isolate (SPI) according to a certain compounding ratio, and carrying out combined modification on the recombined SPI by virtue of modification means such as ultrasonic treatment, heat treatment, magnetic field and enzyme crosslinking; the recombined SPI emulsion is subjected to micronization treatment by adopting high-pressure microjet homogenization treatment, and then the emulsion is jointly induced to be gelatinized by utilizing acid and salt ions and transglutaminase, so that the preparation efficiency, the structural strength and the stability of the emulsion gel are improved; two polyphenols, namely epigallocatechin gallate and quercetin, are co-embedded in an interface and an oil phase of the emulsion gel, so that the bioaccessibility and antioxidant activity of the polyphenols are improved, and the emulsion gel is endowed with functionality. The prepared emulsion gel can be widely applied to processing of functional food.

The present invention relates to the technical field of bacterial enzyme fermentation, and provides a bacterial enzyme synergistic fermentation method for removing antinutritional factors in soybean meal, the method comprises the following steps: S1, soybean meal pretreatment: soybean meal, water and an auxiliary agent are mixed and then subjected to steam explosion treatment, and the auxiliary agent comprises citric acid, a hydrogen peroxide solution and polyphenol; s2, bacterial enzyme fermentation: mixing the pretreated soybean meal with molasses and water to obtain a raw material, adding a complex microbial inoculant and a complex enzyme, uniformly stirring, and fermenting; the complex microbial inoculants comprise saccharomycetes, bacillus subtilis, lactobacillus rhamnosus and pediococcus pentosaceus; the compound enzyme comprises acid protease, pectinase, phytase, glucanase, alpha-galactosidase and keratinase; and S3, after fermentation is finished, drying the materials to obtain the fermented soybean meal. According to the fermentation method, the glycinin degradation rate can reach 79% or above, the acid soluble protein content is increased to 26% or above, the anti-nutritional factor content is remarkably reduced, and the nutritional value is greatly increased.

The invention discloses lysozyme condensed microdroplets as well as a preparation method and application thereof. The lysozyme condensed microdroplet comprises egg white source lysozyme, food protein, edible inorganic salt and water, wherein the food protein comprises at least one of beta-conglycinin and beta-lactoglobulin. The preparation method of the lysozyme condensed microdroplet comprises the following steps: 1) preparing an egg white source lysozyme dispersion liquid and a food protein dispersion liquid; and 2) mixing the two dispersion liquids, and stirring until liquid-liquid phase separation occurs. The lysozyme condensed microdroplet is good in thermodynamic stability and long-term storage stability, the lysozyme is high in mobility in the condensed microdroplet and high in accessibility to a substrate, has long-acting antibacterial and preservative effects, is suitable for being used as a food additive, and is simple in preparation method, green and environment-friendly in process, wide in raw material source, low in production cost, low in energy consumption and suitable for industrial production. The method is suitable for large-scale industrial production and application.

The invention relates to the technical field of microbial fermentation, and discloses bacillus subtilis, application of the bacillus subtilis in fermented soybean meal and a preparation method of the fermented soybean meal. The bacillus subtilis screened by the invention has strong anti-nutritional factor resistance (such as glycinin, beta-conglycinin, raffinose and stachyose), has a good growth effect in the soybean meal raw material, and is used for fermenting the soybean meal raw material; the content of anti-nutritional factors (such as glycinin, beta-conglycinin, raffinose and stachyose) in the soybean meal raw material can be obviously reduced, and meanwhile, the content of lipopeptide substances (such as surfactin and bacitracin D) in the fermented soybean meal product can be obviously improved; the prepared fermented soybean meal product has a good antibacterial effect on three common intestinal pathogens (escherichia coli, salmonella and staphylococcus) in animal bodies.

The invention belongs to the field of water and slurry treatment agents, and discloses a hydrophobic modified glycinin-based flocculant as well as a preparation method and application thereof. The preparation method comprises the following steps: dissociating soybean protein subunits under alkaline and nitrogen protection; pre-emulsified octenyl succinic anhydride or dodecyl glycidyl ether is added for a grafting reaction; adjusting the pH to terminate the reaction and aging; and washing and drying to obtain the product. The flocculant has a hydrophobic association three-dimensional network structure, can efficiently capture particles in water, generates a self-extrusion drainage effect in slurry dehydration, is high in dehydration efficiency and environment-friendly, and is suitable for purification of high-turbidity water and dehydration of building slurry.

The invention provides a preparation method of low-sensitization bean curd, and belongs to the technical field of soybean protein processing. Bean curd is used as healthy food rich in nutrition and contains allergenic proteins such as glycinin and beta-conglycinin, so that eating of part of people is limited, and the problems that the effect is poor and the product quality is influenced exist in an existing allergenic reduction method. Soybeans are used as a raw material, ultrasonic-enzymolysis-lactic acid bacteria fermentation synergistically modifies and degrades sensitization protein, W / O / W multiple emulsion regulates and controls the solidification process, secondary solidification and intelligent pressing optimizes the texture, and ultrahigh pressure cold sterilization synergistically biological preservation is combined, so that the sensitization of the bean curd is remarkably reduced (the reduction rate of the optimal embodiment can reach 68.5%). The preparation method is green and safe in process, the prepared bean curd is low in sensitization and has the advantages of being long in storage period, high in water binding capacity, high in protein digestibility and excellent in flavor, the problems that traditional bean curd is high in sensitization, short in storage period and prone to deterioration in quality are effectively solved, and bean curd eating crowds and application scenes are expanded.

The invention discloses a nano antibody for detecting glycinin, an ELISA kit and application thereof, and belongs to the technical field of glycinin detection. Wherein the nano antibody comprises a nano antibody 1 and a nano antibody 2 which can be respectively used as a coating antibody and a detection antibody (biotin label) in the ELISA kit; the ELISA kit can also comprise reagents such as enzyme-labeled streptavidin, a color developing solution and the like, and the quantitative analysis of the glycinin in a to-be-detected sample is realized by specifically utilizing a sandwich ELISA method and a color developing reaction. According to the scheme, the specificity is high, the detection limit is low, glycinin residues in products such as fermented soybeans, enzymolysis soybeans, soybean meal and feed can be accurately and sensitively detected, meanwhile, the defects that in traditional instrument detection, sample pretreatment is complex, consumed time is long and the like are overcome, and the detection cost is greatly saved.

The invention discloses a method for directionally immobilizing tyrosinase by using magnetic polystyrene nanospheres and application, the method comprises the following steps: firstly, modifying the surfaces of the magnetic polystyrene nanospheres, introducing chloracetyl, then substituting chlorine by using ethylenediamine, covalently grafting concanavalin as a carrier of tyrosinase through amino by using Schiff base reaction, and preparing the magnetic polystyrene nanospheres by using a covalent covalent grafting method. Tyrosinase is immobilized on the surfaces of the magnetic polystyrene nanospheres, directional immobilization of the tyrosinase and efficient enrichment of atrazine are achieved, non-specific binding of trace atrazine in a matrix and enzyme active sites is promoted through the adsorption effect, the activity of the tyrosinase is inhibited, and the activity of atrazine is inhibited. By utilizing the synergistic effect of an enzyme activity indicator catechol and a color indicator 4-aminoantipyrine, the atrazine residues in the complex fruit and vegetable matrix can be rapidly and effectively detected.

To provide a seasoning, sword bean miso, which contains concanavalin A (ConA), a useful component derived from sword beans and expected to improve immunity, and canavalin or its fermentation product, expected to prevent lifestyle-related diseases, and which can be deliciously and reasonably ingested in sufficient amounts. [Solution] By fermenting the legumes of the sword bean using koji mold as a raw material, sword bean miso is brewed, which is rich in useful ingredients such as concanavalin A (ConA) and canavalin, as well as free amino acids that have a delicious flavor and high nutritional value.

The invention relates to a glycinin extract for agriculture and application thereof in agriculture, which is characterized by comprising the steps of globulin extraction and microorganism extraction, the microorganism extracted from the microorganism is bacillus N311 which is preserved in the China Center for Type Culture Collection on October 15, 2015, and the preservation number of the China Center for Type Culture Collection is CCTCC M 2015607; the bacillus N311 is bacillus velezensis, and the bacillus N311 is bacillus velezensis. The glycinin extracting solution prepared by the invention not only has better antibacterial property, but also can promote the growth of crops.

To provide dried noodles that contain high-amylose wheat flour and exhibit reduced powderiness in texture.SOLUTION: A method for producing dried noodles comprises drying raw noodles at 70°C or higher, the raw noodles containing 30 to 100 mass% of high-amylose wheat flour in the total mass of cereal flour and starch, the high-amylose wheat flour being a wheat flour having an amylose content of 40 mass% or more in total starch as analyzed by the Concanavalin A method.SELECTED DRAWING: None

To provide dried noodles that contain high-amylose wheat flour and exhibit good texture.SOLUTION: A method for producing dried noodles comprises drying raw noodles containing 30 mass% or more of high-amylose wheat flour in the total mass of cereal flour and starch, the high-amylose wheat flour being a wheat flour having an amylose content of 40 mass% or more in total starch as analyzed by the Concanavalin A method, and the high-amylose wheat flour having a damaged starch amount of 4.0 to 6.3 mass% as measured by the AACC Method 76-31.SELECTED DRAWING: None

The invention belongs to the technical field of biological fermentation, and particularly relates to a method for synchronously improving the degradation rate and antibacterial activity of antigen protein of fermented soybean meal, a product and application of the product. Three strains with specific functions are combined with a compound enzyme preparation to ferment the soybean meal, on one hand, antigenic factors such as antigen protein in the original soybean meal can be efficiently degraded, the highest degradation rate of glycinin reaches 81.5%, and the degradation rate of beta-conglycinin reaches 78.6%; on the other hand, the fermented soybean meal shows a remarkable antibacterial effect on escherichia coli, salmonella, staphylococcus aureus, aspergillus flavus and the like, and the diameter of an inhibition zone is 28.14-33.17 mm.

Plants producing seeds and seeds comprising increased methionine content are provided. Protein compositions having increased methionine can be used from the seeds. Additionally, methods for generating and using plants, seeds and protein compositions having increased protein content or increased amounts of essential amino acids are disclosed. Methods for generating high methionine variants of seed storage proteins for use in the compositions and methods are also provided.

The invention belongs to the technical field of microorganisms, and particularly relates to a bacillus licheniformis mutant strain for high yield of alkaline protease and application of the bacillus licheniformis mutant strain. The bacillus licheniformis is specifically bacillus licheniformis LKT2025-01, and the preservation number of the bacillus licheniformis LKT2025-01 is CGMCC (China General Microbiological Culture Collection Center) No.36047. The bacillus licheniformis LKT2025-01 and the bacillus licheniformis Alkaline protease produced through liquid fermentation of the strain has a good degradation effect on antigen protein in soybean meal, the degradation rate of glycinin reaches 90%, the degradation rate of beta-conglycinin reaches 80%, toxic and side effects on livestock and poultry are effectively reduced, immunity is enhanced, the health level is improved, and then the growth performance of the livestock and poultry is improved. In addition, the strain is high in temperature resistance, the enzyme activity retention rate is about 85% after the strain is treated at the high temperature of 85 DEG C for 3 min, and the strain can be applied to the feed industry and the livestock and poultry breeding industry.

The invention discloses a method for visually marking transgenic soybean seeds, which comprises the following steps: connecting the front 1000bp nucleotide sequence of a seed-specific promoter pGY1 and ATG of a glycinin gene with an RUBY report system, and inserting the seed-specific promoter pGY1 and ATG into a pMDC123 vector to construct a pMDC123-pGY1:: RUBY recombinant vector; according to the method disclosed by the invention, the transgenic soybean seeds are enabled to present a unique grey green phenotype by utilizing an RUBY report system, and the transgenic seeds can be quickly screened out only through visual inspection without the help of complex instruments and equipment, so that the screening process is greatly simplified, the screening efficiency is improved, and even personnel without professional molecular biology knowledge can also easily operate; the selected pGY1 promoter has the characteristic of seed specificity high expression, which means that the RUBY report system is only expressed in seeds and does not express in other tissues or organs of soybean plants.

The invention belongs to the technical field of soybean protein separation and preparation, and particularly relates to high-purity beta-conglycinin as well as a preparation method and application thereof. The preparation method comprises the following steps: roughly extracting the defatted soybean protein powder, filtering by three layers of gauze to remove coarse particles, optimizing the final concentration of solid NaHSO3, standing for a long time at 4 DEG C for multiple times, and finally freeze-drying to obtain a final product. By optimizing the extraction process route and key parameters, the problems of low product purity and more impurities in the traditional method are effectively solved, and the beta-conglycinin with the purity not lower than 90% is successfully prepared. The method is stable in process and good in repeatability, and a high-quality raw material is provided for application of beta-conglycinin in the fields of high-end food and medicine.

The invention discloses concanavalin A-like agglutinin / glucanase structural domain protein FvCLG and application thereof in improving the yield of fusarium venanii hypha protein, and belongs to the fields of genetic engineering technology and food biotechnology. Compared with a wild type strain, the fusarium venanii FG31 has the advantages that the FvCLG gene on a genome is deleted, ethanol is not generated in a fermentation tank for 72 hours, and the protein content is obviously increased (as high as 61.34%) and is increased by 22.41% compared with the wild type strain. Meanwhile, when the FG31 strain is fermented, the sugar consumption is obviously reduced, the conversion rate from glucose to biomass and the conversion rate from glucose to protein are respectively improved by 44.02% and 76.41%, the utilization rate of a substrate is obviously improved, the loss of a carbon source is reduced, and the production cost is reduced. The fusarium venanii can be used for further developing genetic engineering modification work to cultivate a fusarium venanii strain with high protein content.

The invention discloses pea globulin amyloid fiber / xanthan gum / dietary fiber composite hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: performing first heat treatment on pea globulin under an acidic condition to form a homogeneous core; uniformly mixing the homogeneous core, fresh pea globulin and an exogenous additive, carrying out second heat treatment under an acidic condition, and inducing to form pea globulin amyloid fibers; uniformly mixing the pea globulin amyloid fiber, polysaccharide and dietary fiber, carrying out third heat treatment under an acidic condition, and carrying out heat-induced gelation, so as to obtain the pea globulin amyloid fiber / xanthan gum / dietary fiber composite hydrogel. The composite hydrogel has a ternary interpenetrating network structure synergistically constructed by pea globulin amyloid fiber-xanthan gum-resistant dextrin, realizes an ideal combination of hardness, elasticity and low adhesion, is easy to swallow, and perfectly balances nutrient enrichment and texture maintenance.

This invention relates to the field of microbial enzyme fermentation technology, and proposes a method for synergistic microbial enzyme fermentation to remove anti-nutritional factors from soybean meal, comprising the following steps: S1, pretreatment of soybean meal: soybean meal, water, and auxiliary agents are mixed and subjected to steam explosion treatment, wherein the auxiliary agents include citric acid, hydrogen peroxide solution, and polyphenols; S2, microbial enzyme fermentation: the pretreated soybean meal is mixed with molasses and water to obtain raw material, a compound microbial agent and a compound enzyme are added, and fermentation is carried out after stirring evenly; the compound microbial agent includes yeast, Bacillus subtilis, Lactobacillus rhamnosus, and Pediococcus pentosus; the compound enzyme includes acidic protease, pectinase, phytase, glucanase, α-galactosidase, and keratinase; S3, after fermentation, the material is dried to obtain fermented soybean meal. The fermentation method of this invention can achieve a soybean globulin degradation rate of over 79%, increase the acid-soluble protein content to over 26%, significantly reduce the content of anti-nutritional factors, and greatly improve the nutritional value.

The invention discloses a processing method of high-pressure double-heat-source baked soybeans. The processing method comprises the following steps: S1, preheating a reaction kettle: preheating the reaction kettle through a heating coil assembly; s2, preheating the materials: putting the soybeans into a reaction kettle, and heating the soybeans to 80-95 DEG C within 10-40 minutes; s3, heating and pressurizing: continuously supplying heat by a heating coil assembly, introducing steam into the reaction kettle to be in direct contact with the soybeans, heating the soybeans to 100-140 DEG C, keeping the temperature for 10-50 minutes, and increasing the pressure in the reaction kettle to 0.2-0.7 Mpa; s4, pressure relief: extracting steam in the reaction kettle through a high-pressure fan; s5, performing negative-pressure bursting and dehydration: vacuumizing the reaction kettle through a high-pressure fan, and expanding and bursting the soybeans; and S6, cold-drying and packaging. The method disclosed by the invention has the advantages that urease activity, glycinin and beta-conglycinin in the soybeans can be quickly destroyed at 100-140 DEG C, the protein solubility is improved, and the Maillard reaction is inhibited.

The application belongs to the technical field of feed raw material processing, and particularly relates to a preparation method of low-anti-nutritional factor enzymatic soybean meal. The method comprises the following steps: uniformly mixing crushed soybean meal with an organic mixed acid solution at a material-to-water ratio of 1:0.6-0.9, and obtaining an acidified substrate with a pH of 4.8-5.5 by conditioning at 70-85 DEG C; after cooling to 48-55 DEG C, acid protease and cellulase are added for static hydrolysis for 6-10 h, the acid protease has an enzyme activity retention rate of greater than or equal to 75% under the condition of pH 5.0 and 50 DEG C for 6 h; Bacillus subtilis is gradually dripped with lactic acid for acclimatization to obtain an acid-resistant inoculum that maintains activity at pH 5.2; after cooling, the enzyme hydrolysis substrate is inoculated with the inoculum, and then fermentation is carried out, ammonia is produced by strain metabolism to naturally increase the pH of the material from 4.8-5.5 to 6.2-7.0; after the fermentation is completed, the material is inactivated by airflow and dried. In the method, an acid barrier is established by organic acid pretreatment and the pH interval of enzyme activity is matched, pH self-regulation relay from weak acid enzymolysis to near neutral fermentation is realized by strain acid-tolerant acclimatization, external alkali is not needed for pH adjustment in the whole process, the obtained product contains 20%-35% of small peptides in crude protein, and soybean globulin is less than or equal to 10 mg / g.

This invention provides a Picckering emulsion luminescent aerosol paint for tattoo-like body painting, characterized by comprising the following raw materials in parts by weight: 4-6 parts β-conjugated soy globulin, 0.8-5 parts trisodium citrate, 0.8-4 parts tannic acid, 0.4-2 parts essential oil, 4-6 parts chitosan, 2.4-4 parts luminescent powder, 2.4-4 parts nano silver, 40-50 parts H2O, 6.4-11 parts diluent, 3.2-6 parts colorant, 0.4-2 parts antioxidant, and 15.2-20 parts propellant. This invention also provides a method for preparing the Picckering emulsion luminescent aerosol paint for tattoo-like body painting. Compared with existing technologies, this invention has the following beneficial effects: it can effectively coat luminescent functional fillers, significantly improve the dispersion stability of the system, thereby achieving a good balance between the artistic expression of the paint film and human safety.

The application belongs to the field of food processing, and particularly relates to a method for improving the freezing characteristics of soybean protein isolate plant protein meat. Soybean 7S globulin and soybean 11S globulin are mixed as soybean protein isolate at a 7S / 11S mass ratio of 0.8-1.2:1, other plant proteins and auxiliary materials including TG enzyme high-temperature-resistant microcapsules are compounded, and a high-moisture extrusion process is used for preparation; after forming, heat setting, gradient cooling pretreatment and ultra-high pressure freezing treatment are performed to obtain plant protein meat with good freezing characteristics. The soybean protein isolate plant protein meat prepared by the method has a water holding capacity of 3.5-4.5 g / g, a fiber integrity of 82-95%, an elastic modulus of 250-320 KPa, a freeze-thaw syneresis rate of 15-25%, a gel strength of 35-45 N, a cooking loss rate of 5-15%, and a weakly bound free water proportion of 78-97% after one freeze-thaw cycle.

To provide refrigerated cooked noodles that exhibit improved separability.SOLUTION: There are provided raw noodles for refrigerated cooked noodles and a method for producing refrigerated cooked noodles using the raw noodles. The raw noodle material flour contains 3 mass% or more and less than 20 mass% of high-amylose wheat flour in the total mass of cereal flour and starch, the high-amylose wheat flour being a wheat flour having an amylose content of 40 mass% or more in total starch as analyzed by the Concanavalin A method.SELECTED DRAWING: None

The application discloses a kind of fermented compound feed for preventing piglet diarrhea and its preparation and application method. Corn, soybean meal and bran are premixed according to the mass ratio of 4.5:4.5:1.0, then 10% of ZJUAF-2 fermentation broth and 10% of ZJUAF-4 fermentation broth are added, and 40% of water is added. The initial fermentation temperature is 25-30℃, the fermentation time is 48-72 h, and the material is turned over every 5-8 h. When the pH of the feed is reduced to 4.0, corn-soybean meal type fermented compound feed is obtained. Corn, soybean meal and bran are fermented by mixing one strain of Bacillus subtilis and one strain of Pediococcus pentosaceus. The contents of beta-conglycin and soybean protein decreased by 71.94% and 82.86%, respectively. The content of neutral detergent fiber decreased by 38.93%. The contents of gassy factors raffinose and stachyose decreased by 42.22% and 36.60%, respectively. The content of phytic acid phosphorus decreased by 37.50%. In addition to the significant decrease in the above-mentioned anti-nutritional factors, the lactic acid content increased by 3.5 times, and the acid-soluble protein increased by 6 times. Feeding the fermented feed to piglets can control diarrhea as effectively as feeding antibiotics.

This invention provides a nucleic acid aptamer for recognizing soybean antigen β-conglycinin, the aptamer having the nucleotide sequence shown in SEQ ID NO:1. Using the nucleic acid aptamer of this invention not only exhibits good sensitivity and specificity, but also significantly improves the detection efficiency of soybean antigen β-conglycinin and reduces detection costs. Therefore, this method is suitable for batch detection of soybean antigen proteins in soybeans and their deep-processed products in the food and feed industries.

The invention relates to an Antarctic deep sea salt-resistant rooting bacterium and an application thereof in a fertilizer, and the Antarctic deep sea salt-resistant rooting bacterium is characterized by comprising globulin extraction and microorganism extraction, the microorganism extracted from the microorganism is bacillus N311 which is preserved in the China Center for Type Culture Collection on October 15, 2015, and the preservation number of the China Center for Type Culture Collection is CCTCC M 2015607; the bacillus N311 is bacillus velezensis, and the bacillus N311 is bacillus velezensis. The glycinin extracting solution prepared by the invention not only has better antibacterial property, but also can promote the growth of crop roots.