Pesticide preparation for pepper diseases and application thereof

Abstract

The invention discloses a pesticide preparation for pepper diseases and application of the pesticide preparation, active ingredients of the pesticide preparation comprise carvacrol and beta-lupin globulin polypeptide, and the mass ratio of the carvacrol to the beta-lupin globulin polypeptide is 1: 9-1: 1. According to the invention, the botanical pesticide carvacrol and the polypeptide biological fungicide beta-lupin globulin polypeptide are compounded, so that a synergistic interaction effect is achieved, the defect that chemical pesticides are easy to generate residues is overcome, and the pesticide is a novel pesticide which is efficient, free of residues and safe.

Description

Technical Field

[0001] The invention relates to the technical field of pesticides, and in particular to a pesticide preparation for pepper diseases and application thereof. Background Art

[0002] Pepper is an annual or limited perennial herb. The fruit of pepper has a spicy taste because the peel contains capsaicin, which can increase appetite. The content of vitamin C in pepper ranks first among vegetables. Pepper is one of the main economic crops in my country. With the continuous expansion of pepper planting area, pepper diseases, especially leaf diseases, have shown a significant trend of aggravation, which has a serious impact on pepper yield and quality. Pepper anthracnose and powdery mildew are common fungal diseases on pepper leaves.

[0003] At present, there are two main ways to prevent and control pepper leaf diseases: first, agricultural prevention, selecting disease-resistant varieties, strengthening field management, etc., but the prevention and control effect often does not meet expectations; second, chemical prevention, spraying peppers with pesticides to inhibit pathogens, but most of the pesticides used are chemical agents, which have the risk of excessive residues. Therefore, it is urgent to develop efficient, residue-free, and pollution-free pesticides for pepper disease prevention and control.

[0004] Carvacrol is a botanical fungicide that can effectively inhibit the germination of pathogenic bacteria spores and the growth of mycelium. Its mechanism of action is to quickly increase the permeability and osmotic pressure of the cell membrane, causing the pathogen cell membrane to rupture and the leakage of intracellular substances, thereby killing the pathogen. It can effectively prevent and treat diseases such as tomato gray mold, potato late blight and tobacco brown spot disease.

[0005] β-Lupin Globulin Peptide is a new type of biofungicide, a naturally occurring peptide formed during the germination of edible sweet lupin seeds. The mechanism of action is a combined action on several target sites of fungal cells, and the chelation activity of several cations can interfere with the homeostasis of microbial cells and ultimately lead to cell death. The mode of action is a protective biofungicide, which has a good control effect on strawberry gray mold and grape powdery mildew.

[0006] There is no report on the combination of carvacrol and β-lupin globulin polypeptide. Summary of the invention

[0007] In view of the above-mentioned prior art, the purpose of the present invention is to provide a pesticide preparation for pepper diseases and its application. The present invention uses carvacrol and β-lupin globulin polypeptide as active ingredients, wherein carvacrol is a plant-derived pesticide and β-lupin globulin polypeptide is a new type of biological fungicide. The combination of the two has excellent control effects on pepper diseases, and is residue-free and pollution-free.

[0008] To achieve the above object, the present invention adopts the following technical solution:

[0009] A first aspect of the present invention provides a pesticide preparation for pepper diseases, wherein the active ingredients of the pesticide preparation consist of carvacrol and β-lupin globulin polypeptide, and the mass ratio of the carvacrol to the β-lupin globulin polypeptide is 1:9 to 1:1.

[0010] Preferably, the mass ratio of carvacrol to β-lupin globulin polypeptide is 1:3.

[0011] Preferably, the mass fraction of the carvacrol and β-lupin globulin polypeptide in the pesticide preparation is 3-30%; the pesticide also includes an adjuvant and a solvent.

[0012] More preferably, the auxiliary agent is composed of alkyl glycoside, polycarboxylate and polyether modified polysiloxane in a mass ratio of 1 to 6:1 to 6:0.2.

[0013] The second aspect of the present invention provides the use of the above-mentioned pesticide preparation for pepper diseases in preventing and controlling pepper diseases.

[0014] Preferably, the pepper disease is anthracnose.

[0015] Beneficial effects of the present invention:

[0016] The present invention compounds the plant-derived pesticide carvacrol and the polypeptide biofungicide β-lupin globulin polypeptide, has a synergistic effect, overcomes the defect that chemical pesticides easily produce residues, and is a highly efficient, residue-free and safe new pesticide. DETAILED DESCRIPTION

[0017] The technical scheme of the present invention is described below in conjunction with the embodiments, but the present invention is not limited to the following embodiments. The experimental methods and detection methods described in each embodiment are conventional methods unless otherwise specified; the reagents and materials described are all commercially available unless otherwise specified.

[0018] Formulation Examples

[0019] Example 1: 30% carvacrol·β-lupin globulin polypeptide aqueous solution

[0020] Ingredients: 3g carvacrol, 27g β-lupin globulin polypeptide, 6g alkyl glycoside, 6g polycarboxylate, 0.2g polyether modified polysiloxane, 7.2g ethanol, and 50.6g water.

[0021] Example 2: 20% carvacrol·β-lupin globulin polypeptide aqueous solution

[0022] Ingredients: 5g carvacrol, 15g β-lupin globulin polypeptide, 4g alkyl glycoside, 4g polycarboxylate, 0.2g polyether modified polysiloxane, 12g ethanol, and 59.8g water.

[0023] Example 3: 8% carvacrol·β-lupin globulin polypeptide aqueous solution

[0024] Ingredients: 4g carvacrol, 4g β-lupin globulin polypeptide, 3g alkyl glycoside, 3g polycarboxylate, 0.2g polyether modified polysiloxane, 9.6g ethanol, and 76.2g water.

[0025] Example 4: 3% carvacrol·β-lupin globulin polypeptide aqueous solution

[0026] Ingredients: 1g carvacrol, 2g β-lupin globulin polypeptide, 1g alkyl glycoside, 1g polycarboxylate, 0.2g polyether modified polysiloxane, 2.4g ethanol, and 92.4g water.

[0027] Comparative Example 1: 5% carvacrol aqueous solution

[0028] Ingredients: 5g carvacrol, 1.5g alkyl glycoside, 1.5g polycarboxylate, 0.2g polyether modified polysiloxane, 12g ethanol, and 79.8g water.

[0029] Comparative Example 2: 20% β-lupin globulin polypeptide aqueous solution

[0030] Ingredients: 20g β-lupin globulin polypeptide, 4g alkyl glycoside, 4g polycarboxylate, 0.2g polyether modified polysiloxane, and 71.8g water.

[0031] Comparative Example 3: 4% carvacrol·β-lupin globulin polypeptide aqueous solution

[0032] Ingredients: 3g carvacrol, 1g β-lupin globulin polypeptide, 1.5g alkyl glycoside, 1.5g polycarboxylate, 0.2g polyether modified polysiloxane, 7.2g ethanol, and 85.6g water.

[0033] Indoor toxicity test example

[0034] The test agent carvacrol was dissolved in ethanol, and β-lupin globulin polypeptide was dissolved in distilled water, and then diluted with 0.1% Tween-80 aqueous solution to prepare single-dose stock solutions and mixed stock solutions with different mass ratios (1:27, 1:9, 1:3, 1:2, 1:1, 2:1, 3:1, 9:1, 27:1). Five mass concentration gradients were set for each single agent and mixture by the equal ratio method.

[0035] The mycelium growth rate method was used to determine the toxicity of each single agent and mixture to Colletotrichum oxysporum (collected from pepper plants that had already shown anthracnose in the pepper greenhouse of Sanqu Town, Jingyang County, Shaanxi Province, separated and purified in the laboratory, and the pathogen was identified as Colletotrichum oxysporum and stored on PDA medium). Under sterile conditions, 1 mL of the above gradient solution was added to the sterilized culture dish, and then 14 mL of the melted PDA medium cooled to 50°C was added, and the mixture was shaken thoroughly. Equal amounts of water and PDA medium were used as blank control groups, and each treatment group was repeated 3 times. A 5mm punch was used to punch the edge of the normally growing strain colony to obtain a bacterial cake with consistent growth, which was inoculated into a flat plate, one per plate, with the mycelium side facing down, and cultured in a 25°C incubator. The mycelium growth was observed regularly, and the colony diameter was measured by the cross method after several days of culture to calculate the mycelium growth inhibition rate. The effective inhibition concentration (EC) of each agent was calculated based on the regression analysis between the logarithm of the agent concentration (x) and the corresponding inhibition rate (Y). 50 ), calculate the expected effective inhibitory concentration (EC 50exp ) and the SR, the calculation results are shown in Table 1.

[0036] Inhibition rate (%) = [(colony diameter of control group - colony diameter of treatment group) / (colony diameter of control group - diameter of puncher)] × 100

[0037] EC 50exp =(a+b) / [(a / EC 50a )+(b / EC 50b )]

[0038] SR=EC 50exp / EC 50实际

[0039] In the formula, a is the percentage of carvacrol in the mixture, b is the percentage of β-lupin globulin polypeptide in the mixture, EC 50a is the effective inhibitory concentration of carvacrol (a) when used alone, EC 50b It is the effective inhibitory concentration of β-lupin globulin polypeptide (b) when used alone. SR ≥ 1.5 is a synergistic effect, SR ≤ 0.5 is an antagonistic effect, and 0.5 < SR < 1.5 is an additive effect.

[0040] Table 1: Inhibitory effect of agricultural composition on hyphae growth of Colletotrichum oxysporum

[0041]

[0042] As shown in Table 1, when the mass ratio of carvacrol and β-lupin globulin polypeptide was in the range of 1:9 to 1:1, it had an excellent inhibitory effect on the hyphae growth of Colletotrichum oxysporum, and the SR was greater than 1.5, showing a synergistic effect. Especially when the mass ratio of carvacrol and β-lupin globulin polypeptide was 1:3, the SR was 3.36, and the synergistic effect was optimal.

[0043] Field efficacy test example

[0044] The test site is located in the pepper greenhouse in Sanqu Town, Jingyang County, Shaanxi Province, where the anthrax disease is more serious.

[0045] The experiment was divided into 7 treatment groups: Example 1, Example 2, Example 3, Example 4, Comparative Example 1, Comparative Example 2 and Comparative Example 3; the effective ingredient application rate was 5 g / mu. The area of ​​the plot was 30 square meters, each treatment group was repeated 3 times, and the blank control group was not treated with any agent, but only with clean water.

[0046] The pesticide was applied at the initial onset of pepper anthracnose using conventional spraying methods, with the pesticide formulations of each treatment group sprayed evenly on the pepper plants until droplets appeared; the spraying dose for each treatment group was the same; spraying was repeated at an interval of 7 days, for two consecutive sprayings.

[0047] The disease was investigated 10 days after the second application of pesticides. 50 fruits were randomly investigated in each plot and graded according to the percentage of the lesion area to the total fruit area:

[0048] Grading method:

[0049] Grade 0, no lesions;

[0050] Level 1: the lesion area accounts for less than 2% of the fruit area;

[0051] Level 3: the lesion area accounts for 3% to 8% of the fruit area;

[0052] Level 5: the lesion area accounts for 9% to 15% of the fruit area;

[0053] Level 7: the lesion area accounts for 16% to 25% of the fruit area;

[0054] Level 9: The lesion area accounts for more than 25% of the fruit area.

[0055] The test results are shown in Table 2.

[0056] Disease index = ∑ (number of diseased fruits at each level × relative level value) × 100 / (total number of investigated fruits × 9)

[0057] Control effect = (disease index of blank control area after pesticide application - disease index of drug treatment area after pesticide application) × 100 / disease index of blank control area after pesticide application

[0058] Table 2: Effect of pesticide preparations on pepper anthracnose

[0059]

[0060] As shown in Table 2, when the mass ratio of carvacrol and β-lupin globulin polypeptide is in the range of 1:9 to 1:1, the control effect on pepper anthracnose is 70.8-83.9%; when carvacrol or β-lupin globulin polypeptide is used alone, the control effect on pepper anthracnose is 60.2% and 38.7%; when the mass ratio range provided by the present invention is exceeded, the control effect of the composition on pepper anthracnose is 56.5%. The above results show that the composition of carvacrol and β-lupin globulin polypeptide provided by the present invention within the ratio range has a good effect on preventing and controlling pepper anthracnose, and the control effect is significantly better than any single agent. When the ratio range provided by the present application is exceeded, the control effect becomes worse.

[0061] It can be seen from the above experimental results that the pesticide preparation of the present invention has an excellent control effect on pepper anthracnose.

[0062] The embodiments described above are part of the embodiments of the present invention, but not all of them. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but only represents selected embodiments of the present invention. All other embodiments obtained without creative work and related deductions and substitutions made by ordinary technicians in the field under the conditions of the concept of the present invention belong to the scope of protection of the present invention.

Claims

1. A pesticide preparation for pepper diseases, wherein the active ingredients of the pesticide preparation are composed of carvacrol and β-lupin globulin polypeptide, and the mass ratio of the carvacrol to the β-lupin globulin polypeptide is 1:9 to 1:

1.

2. A pesticide preparation for pepper diseases according to claim 1, characterized in that: The mass ratio of carvacrol to β-lupin globulin polypeptide is 1:

3.

3. A pesticide preparation for pepper diseases according to claim 1, characterized in that: The mass fraction of the carvacrol and beta-lupin globulin polypeptide in the pesticide preparation is 3-30%; the pesticide also includes an adjuvant and a solvent.

4. A pesticide preparation for pepper diseases according to claim 3, characterized in that: The auxiliary agent is composed of alkyl polyglycoside, polycarboxylate and polyether modified polysiloxane in a mass ratio of 1-6:1-6:0.

2.

5. Use of the pesticide preparation for pepper diseases according to any one of claims 1 to 4 in preventing and controlling pepper diseases.

6. Use of the pesticide preparation for pepper diseases according to claim 5 in preventing and controlling pepper diseases, characterized in that: The pepper disease is anthracnose.

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